Literature DB >> 3277625

Subcellular localization of two porphyrin-synthesis enzymes in Pisum sativum (pea) and Arum (cuckoo-pint) species.

A G Smith1.   

Abstract

The subcellular location of the two porphyrin-synthesis enzymes 5-aminolaevulinate dehydratase (ALAD) and porphobilinogen deaminase (PBGD) was investigated in Pisum sativum (pea) leaves and spadices of Arum (cuckoo-pint). Throughout the tissue-fractionation procedures the distribution of the two enzymes paralleled that of the plastid marker enzyme (ADP-glucose pyrophosphorylase), even in Arum, a tissue where the synthesis of non-plastid haem is predominant. The distribution of cytosolic marker enzyme (lactate dehydrogenase) was significantly different from that of ALAD and PBGD and, although purified mitochondria from both species had some residual activity, this was always less than contaminating plastid marker enzyme. The results suggest that ALAD and PBGD are exclusively plastid enzymes. The significance of this for the role of plastids in cellular porphyrin synthesis is discussed.

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Year:  1988        PMID： 3277625      PMCID： PMC1148720          DOI： 10.1042/bj2490423

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  11 in total

1.  COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

Authors:  D I Arnon
Journal:  Plant Physiol       Date:  1949-01       Impact factor: 8.340

2.  Properties of substantially chlorophyll-free pea leaf mitochondria prepared by sucrose density gradient separation.

Authors:  D Nash; J T Wiskich
Journal:  Plant Physiol       Date:  1983-03       Impact factor: 8.340

3.  The subcellular loclization and properties of the ferrochelatase of etiolated barley.

Authors:  H N Little; O T Jones
Journal:  Biochem J       Date:  1976-05-15       Impact factor: 3.857

4.  Oxidation of protoporphyrinogen to protoporphyrin, a step in chlorophyll and haem biosynthesis. Purification and partial characterization of the enzyme from barley organelles.

Authors:  J M Jacobs; N J Jacobs
Journal:  Biochem J       Date:  1987-05-15       Impact factor: 3.857

5.  Biosynthesis of porphyrins in wheat leaves. II. 5-aminolaevulinate hydro-lyase.

Authors:  D L Nandi; E R Waygood
Journal:  Can J Biochem       Date:  1967-02

6.  Protoheme turnover and chlorophyll synthesis in greening barley tissue.

Authors:  P A Castelfranco; O T Jones
Journal:  Plant Physiol       Date:  1975-03       Impact factor: 8.340

7.  Role and location of NAD malic enzyme in thermogenic tissues of Araceae.

Authors:  T ap Rees; J H Bryce; P M Wilson; J H Green
Journal:  Arch Biochem Biophys       Date:  1983-12       Impact factor: 4.013

8.  Coproporphyrinogenase in tobacco (Nicotiana tabacum L.).

Authors:  W P Hsu; G W Miller
Journal:  Biochem J       Date:  1970-04       Impact factor: 3.857

9.  Purification and properties of L(+)-lactate dehydrogenase from potato tubers.

Authors:  D D Davies; S Davies
Journal:  Biochem J       Date:  1972-10       Impact factor: 3.857

Review 10.  Transport of proteins into mitochondria and chloroplasts.

Authors:  N H Chua; G W Schmidt
Journal:  J Cell Biol       Date:  1979-06       Impact factor: 10.539
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  27 in total

1.  Measurement of ferrochelatase activity using a novel assay suggests that plastids are the major site of haem biosynthesis in both photosynthetic and non-photosynthetic cells of pea (Pisum sativum L.).

Authors:  Johanna E Cornah; Jennifer M Roper; Davinder Pal Singh; Alison G Smith
Journal:  Biochem J       Date:  2002-03-01       Impact factor: 3.857

2.  Differential expression of two hemA mRNAs encoding glutamyl-tRNA reductase proteins in greening cucumber seedlings.

Authors:  R Tanaka; K Yoshida; T Nakayashiki; T Masuda; H Tsuji; H Inokuchi; A Tanaka
Journal:  Plant Physiol       Date:  1996-04       Impact factor: 8.340

3.  Pollen-derived rice calli that have large deletions in plastid DNA do not require protein synthesis in plastids for growth.

Authors:  T Harada; R Ishikawa; M Niizeki; K Saito
Journal:  Mol Gen Genet       Date:  1992-05

Review 4.  Heme synthesis in the rhizobium-legume symbiosis: a palette for bacterial and eukaryotic pigments.

Authors:  M R O'Brian
Journal:  J Bacteriol       Date:  1996-05       Impact factor: 3.490

5.  Porphyrin Accumulation and Export by Isolated Barley (Hordeum vulgare) Plastids (Effect of Diphenyl Ether Herbicides).

Authors:  J. M. Jacobs; N. J. Jacobs
Journal:  Plant Physiol       Date:  1993-04       Impact factor: 8.340

Review 6.  Porphobilinogen synthase, the first source of heme's asymmetry.

Authors:  E K Jaffe
Journal:  J Bioenerg Biomembr       Date:  1995-04       Impact factor: 2.945

7.  Isolation, sequencing and expression of cDNA sequences encoding uroporphyrinogen decarboxylase from tobacco and barley.

Authors:  H P Mock; L Trainotti; E Kruse; B Grimm
Journal:  Plant Mol Biol       Date:  1995-05       Impact factor: 4.076

8.  Subcellular location of the tetrapyrrole synthesis enzyme porphobilinogen deaminase in higher plants: an immunological investigation.

Authors:  M Witty; R M Jones; M S Robb; P M Jordan; A G Smith
Journal:  Planta       Date:  1996       Impact factor: 4.116

9.  Reduction of Uroporphyrinogen Decarboxylase by Antisense RNA Expression Affects Activities of Other Enzymes Involved in Tetrapyrrole Biosynthesis and Leads to Light-Dependent Necrosis.

Authors:  H. P. Mock; B. Grimm
Journal:  Plant Physiol       Date:  1997-04       Impact factor: 8.340

10.  Porphobilinogen deaminase is encoded by a single gene in Arabidopsis thaliana and is targeted to the chloroplasts.

Authors:  S H Lim; M Witty; A D Wallace-Cook; L I Ilag; A G Smith
Journal:  Plant Mol Biol       Date:  1994-11       Impact factor: 4.076
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