Literature DB >> 3277618

Identification by fast atom bombardment mass spectrometry of insulin fragments produced by insulin proteinase.

L A Savoy1, R M Jones, S Pochon, J G Davies, A V Muir, R E Offord, K Rose.   

Abstract

We describe the isolation by reversed-phase h.p.l.c. of a number of products of the degradation of insulin by insulin proteinase and their direct analysis by fast atom bombardment mass spectrometry (f.a.b.-m.s.). Various semisynthetically labelled insulins were used, including [[2H2]GlyA1]insulin and [18O]LysB29]insulin. The results obtained confirm and extend the results obtained by non-mass-spectrometric methods [Davies, Muir, Rose & Offord (1988) Biochem. J. 249, 209-214, and papers cited therein]. Cleavage sites were identified between positions A13-A14, A14-A15, B9-B10, B13-B14, B24-B25 and B25-B26. The advantages and disadvantages of the application of f.a.b.-m.s. to such studies are discussed.

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Year:  1988        PMID： 3277618      PMCID： PMC1148687          DOI： 10.1042/bj2490215

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  9 in total

1.  High performance liquid chromatographic analysis of insulin degradation by rat skeletal muscle insulin protease.

Authors:  F G Hamel; D E Peavy; M P Ryan; W C Duckworth
Journal:  Endocrinology       Date:  1986-01       Impact factor: 4.736

2.  Identification of some cleavage sites of insulin by insulin proteinase.

Authors:  J G Davies; A V Muir; R E Offord
Journal:  Biochem J       Date:  1986-12-01       Impact factor: 3.857

3.  The identification of a major product of the degradation of insulin by 'insulin proteinase' (EC 3.4.22.11).

Authors:  A Muir; R E Offord; J G Davies
Journal:  Biochem J       Date:  1986-08-01       Impact factor: 3.857

4.  Identification of an insulin fragment produced by an insulin degrading enzyme, neutral thiopeptidase.

Authors:  P T Varandani; L A Shroyer
Journal:  Mol Cell Endocrinol       Date:  1987-04       Impact factor: 4.102

5.  A mass-spectrometric investigation of the mechanism of the semisynthetic transformation of pig insulin into an ester of insulin of human sequence.

Authors:  K Rose; J Gladstone; R E Offord
Journal:  Biochem J       Date:  1984-05-15       Impact factor: 3.857

6.  Fast-atom-bombardment mass spectrometry. A new technique for the determination of molecular weights and amino acid sequences of peptides.

Authors:  D H Williams; C V Bradley; S Santikarn; G Bojesen
Journal:  Biochem J       Date:  1982-01-01       Impact factor: 3.857

7.  Initial site of insulin cleavage by insulin protease.

Authors:  W C Duckworth; F B Stentz; M Heinemann; A E Kitabchi
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

8.  The preparation of tritiated insulin specifically labelled by semisynthesis at glycine-A1.

Authors:  J G Davies; R E Offord
Journal:  Biochem J       Date:  1985-10-15       Impact factor: 3.857

9.  Identification of radioactive insulin fragments liberated by insulin proteinase during the degradation of semisynthetic [3H]GlyA1]insulin and [3H]PheB1]insulin.

Authors:  J G Davies; A V Muir; K Rose; R E Offord
Journal:  Biochem J       Date:  1988-01-01       Impact factor: 3.857
  9 in total
  6 in total

1.  Partial characterization of natural and recombinant human soluble CD23.

Authors:  K Rose; G Turcatti; P Graber; S Pochon; P O Regamey; K U Jansen; E Magnenat; N Aubonney; J Y Bonnefoy
Journal:  Biochem J       Date:  1992-09-15       Impact factor: 3.857

2.  Characterization of recombinant-derived granulocyte-colony stimulating factor (G-CSF).

Authors:  P Wingfield; R Benedict; G Turcatti; B Allet; J J Mermod; J DeLamarter; M G Simona; K Rose
Journal:  Biochem J       Date:  1988-11-15       Impact factor: 3.857

3.  C-terminal peptide identification by fast atom bombardment mass spectrometry.

Authors:  K Rose; L A Savoy; M G Simona; R E Offord; P Wingfield
Journal:  Biochem J       Date:  1988-02-15       Impact factor: 3.857

4.  Preparation and characterization of novel substrates of insulin proteinase (EC 3.4.99.45).

Authors:  R C Werlen; R E Offord; K Rose
Journal:  Biochem J       Date:  1994-09-15       Impact factor: 3.857

5.  Identification of radioactive insulin fragments liberated by insulin proteinase during the degradation of semisynthetic [3H]GlyA1]insulin and [3H]PheB1]insulin.

Authors:  J G Davies; A V Muir; K Rose; R E Offord
Journal:  Biochem J       Date:  1988-01-01       Impact factor: 3.857

6.  Insulin proteinase liberates from glucagon a fragment known to have enhanced activity against Ca2+ + Mg2+-dependent ATPase.

Authors:  K Rose; L A Savoy; A V Muir; J G Davies; R E Offord; G Turcatti
Journal:  Biochem J       Date:  1988-12-15       Impact factor: 3.857
  6 in total

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