Literature DB >> 32770512

Characterization of Lipid Order and Domain Formation in Model Membranes Using Fluorescence Microscopy and Spectroscopy.

Andrew Fuhrer1, Amir M Farnoud2,3.   

Abstract

Fluorescence-based techniques have been an integral factor in the study of cellular and model membranes. Fluorescence studies carried out on model membranes have provided valuable structural information and have helped reveal mechanistic detail regarding the formation and properties of ordered lipid domains, commonly known as lipid rafts. This chapter focuses on four techniques, based on fluorescence spectroscopy or microscopy, which are commonly used to analyze lipid rafts. The techniques described in this chapter may be used in a variety of ways and in combination with other techniques to provide valuable information regarding lipid order and domain formation, especially in model membranes.

Entities:  

Keywords:  FRET; Fluorescence anisotropy; Giant unilamellar vesicles; Lipid domains; Model membranes; Vesicle integrity

Mesh：

Substances：

Year:  2021        PMID： 32770512      PMCID： PMC8725914          DOI： 10.1007/978-1-0716-0814-2_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  11 in total

Review 1.  Structure and function of sphingolipid- and cholesterol-rich membrane rafts.

Authors:  D A Brown; E London
Journal:  J Biol Chem       Date:  2000-06-09       Impact factor: 5.157

Review 2.  The renaissance of fluorescence resonance energy transfer.

Authors:  P R Selvin
Journal:  Nat Struct Biol       Date:  2000-09

Review 3.  Functions of lipid rafts in biological membranes.

Authors:  D A Brown; E London
Journal:  Annu Rev Cell Dev Biol       Date:  1998       Impact factor: 13.827

4.  Sterol structure determines the separation of phases and the curvature of the liquid-ordered phase in model membranes.

Authors:  Kirsten Bacia; Petra Schwille; Teymuras Kurzchalia
Journal:  Proc Natl Acad Sci U S A       Date:  2005-02-18       Impact factor: 11.205

5.  Formation of unilamellar vesicles by repetitive freeze-thaw cycles: characterization by electron microscopy and 31P-nuclear magnetic resonance.

Authors:  M Traïkia; D E Warschawski; M Recouvreur; J Cartaud; P F Devaux
Journal:  Eur Biophys J       Date:  2000       Impact factor: 1.733

6.  Measurement of lipid nanodomain (raft) formation and size in sphingomyelin/POPC/cholesterol vesicles shows TX-100 and transmembrane helices increase domain size by coalescing preexisting nanodomains but do not induce domain formation.

Authors:  Priyadarshini Pathak; Erwin London
Journal:  Biophys J       Date:  2011-11-15       Impact factor: 4.033

7.  Effect of the structure of lipids favoring disordered domain formation on the stability of cholesterol-containing ordered domains (lipid rafts): identification of multiple raft-stabilization mechanisms.

Authors:  Omar Bakht; Priyadarshini Pathak; Erwin London
Journal:  Biophys J       Date:  2007-08-31       Impact factor: 4.033

8.  TMA-DPH: a suitable fluorescence polarization probe for specific plasma membrane fluidity studies in intact living cells.

Authors:  J G Kuhry; P Fonteneau; G Duportail; C Maechling; G Laustriat
Journal:  Cell Biophys       Date:  1983-06

9.  Structural order of lipids and proteins in membranes: evaluation of fluorescence anisotropy data.

Authors:  F Jähnig
Journal:  Proc Natl Acad Sci U S A       Date:  1979-12       Impact factor: 11.205

10.  Electroformation of Giant Unilamellar Vesicles on Stainless Steel Electrodes.

Authors:  Valerio Pereno; Dario Carugo; Luca Bau; Erdinc Sezgin; Jorge Bernardino de la Serna; Christian Eggeling; Eleanor Stride
Journal:  ACS Omega       Date:  2017-03-16
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