Jingjing Mei1, Yuan Zhang2, Shuangshuang Lu3, Jing Wang4. 1. Department of Respiratory and Critical Diseases Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052 Henan, China. 2. Laboratory Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052 Henan, China. 3. National Center for International Research in Cell and Gene Therapy, Sino-British Research Centre for Molecular Oncology, Basic Medical School, Zhengzhou University, Zhengzhou, 450052, China. 4. Department of Respiratory and Critical Diseases Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052 Henan, China. Electronic address: jingluanben88@163.com.
Abstract
BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a kind of chronic lung disease that mainly induced by smoking-caused inflammation. Long non-coding RNAs (lncRNAs) have been reported to play a part in the course of pulmonary diseases. Here, we studied the role of lncRNA NNT-AS1 in the development of COPD. MATERIALS: qRT-PCR analysis and ELISA assay were applied to evaluate the expression of genes and inflammatory cytokines, respectively. CCK8 and EdU assays were utilized to assess proliferation, while flow cytometry assay was conducted to evaluate apoptosis. Luciferase reporter, RNA pull down and RIP assays were combined to explore relationships between genes. RESULTS: NNT-AS1 was observed to be up-regulated in cigarette smoke extract (CSE)-treated 16HBE cells. Knockdown of NNT-AS1 abolished CSE-caused suppressive effects on cell proliferation, apoptosis, inflammation and airway remodeling. Mechanistically, NNT-AS1 up-regulated FBXO11 expression via sponging miR-582-5p. Moreover, miR-582-5p inhibitor or FBXO11 overexpression counteracted NNT-AS1 silence-elicited effects on proliferation, apoptosis, inflammation and airway remodeling. CONCLUSION: Our data revealed that NNT-AS1 played a promoting role in smoking-induced COPD via modulating miR-582-5p/FBXO11 signaling, suggesting a novel potential target for COPD treatment.
BACKGROUND:Chronic obstructive pulmonary disease (COPD) is a kind of chronic lung disease that mainly induced by smoking-caused inflammation. Long non-coding RNAs (lncRNAs) have been reported to play a part in the course of pulmonary diseases. Here, we studied the role of lncRNA NNT-AS1 in the development of COPD. MATERIALS: qRT-PCR analysis and ELISA assay were applied to evaluate the expression of genes and inflammatory cytokines, respectively. CCK8 and EdU assays were utilized to assess proliferation, while flow cytometry assay was conducted to evaluate apoptosis. Luciferase reporter, RNA pull down and RIP assays were combined to explore relationships between genes. RESULTS:NNT-AS1 was observed to be up-regulated in cigarette smoke extract (CSE)-treated 16HBE cells. Knockdown of NNT-AS1 abolished CSE-caused suppressive effects on cell proliferation, apoptosis, inflammation and airway remodeling. Mechanistically, NNT-AS1 up-regulated FBXO11 expression via sponging miR-582-5p. Moreover, miR-582-5p inhibitor or FBXO11 overexpression counteracted NNT-AS1 silence-elicited effects on proliferation, apoptosis, inflammation and airway remodeling. CONCLUSION: Our data revealed that NNT-AS1 played a promoting role in smoking-induced COPD via modulating miR-582-5p/FBXO11 signaling, suggesting a novel potential target for COPD treatment.
Authors: Marko Manevski; Dinesh Devadoss; Christopher Long; Shashi P Singh; Mohd Wasim Nasser; Glen M Borchert; Madhavan N Nair; Irfan Rahman; Mohan Sopori; Hitendra S Chand Journal: Front Immunol Date: 2022-06-14 Impact factor: 8.786