| Literature DB >> 32768389 |
Yongli Shan1, John H Abel2, Yan Li1, Mariko Izumo1, Kimberly H Cox1, Byeongha Jeong1, Seung-Hee Yoo1, David P Olson3, Francis J Doyle4, Joseph S Takahashi5.
Abstract
The suprachiasmatic nucleus (SCN) acts as a master pacemaker driving circadian behavior and physiology. Although the SCN is small, it is composed of many cell types, making it difficult to study the roles of particular cells. Here we develop bioluminescent circadian reporter mice that are Cre dependent, allowing the circadian properties of genetically defined populations of cells to be studied in real time. Using a Color-Switch PER2::LUCIFERASE reporter that switches from red PER2::LUCIFERASE to green PER2::LUCIFERASE upon Cre recombination, we assess circadian rhythms in two of the major classes of peptidergic neurons in the SCN: AVP (arginine vasopressin) and VIP (vasoactive intestinal polypeptide). Surprisingly, we find that circadian function in AVP neurons, not VIP neurons, is essential for autonomous network synchrony of the SCN and stability of circadian rhythmicity.Entities:
Keywords: Per2 gene; SCN; bioluminescence imaging; circadian rhythms; luciferase; neuronal coupling; neuronal network; suprachiasmatic nucleus
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Year: 2020 PMID： 32768389 DOI： 10.1016/j.neuron.2020.07.012
Source DB: PubMed Journal: Neuron ISSN： 0896-6273 Impact factor: 17.173