Zelin Tian1, Weixiang He2, Jianing Tang1, Xing Liao1, Qian Yang1, Yumin Wu1, Gaosong Wu1. 1. Department of Thyroid and Breast Surgery, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China. 2. Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.
Abstract
INTRODUCTION: Breast cancer (BRCA) has the highest incidence among female malignancies, and the prognosis for these patients remains poor. MATERIALS AND METHODS: In this study, core modules and central genes related to BRCA were identified through a weighted gene co-expression network analysis (WGCNA). Gene expression profiles and clinical data of GSE25066 were obtained from the Gene Expression Omnibus (GEO) database. The result was validated with RNA-seq data from The Cancer Genome Atlas (TCGA) and Oncomine database. The top 30 key module genes with the highest intramodule connectivity were selected as the core genes (R2 = 0.40). RESULTS: According to TCGA and Oncomine datasets, seven genes were selected as candidate hub genes. Following further experimental verification, four hub genes (FAM171A1, NDFIP1, SKP1, and REEP5) were retained. CONCLUSION: We identified four hub genes as candidate biomarkers for BRCA. These hub genes may provide a theoretical basis for targeted therapy against BRCA.
INTRODUCTION: Breast cancer (BRCA) has the highest incidence among female malignancies, and the prognosis for these patients remains poor. MATERIALS AND METHODS: In this study, core modules and central genes related to BRCA were identified through a weighted gene co-expression network analysis (WGCNA). Gene expression profiles and clinical data of GSE25066 were obtained from the Gene Expression Omnibus (GEO) database. The result was validated with RNA-seq data from The Cancer Genome Atlas (TCGA) and Oncomine database. The top 30 key module genes with the highest intramodule connectivity were selected as the core genes (R2 = 0.40). RESULTS: According to TCGA and Oncomine datasets, seven genes were selected as candidate hub genes. Following further experimental verification, four hub genes (FAM171A1, NDFIP1, SKP1, and REEP5) were retained. CONCLUSION: We identified four hub genes as candidate biomarkers for BRCA. These hub genes may provide a theoretical basis for targeted therapy against BRCA.
Authors: Jason Howitt; Jenny Lackovic; Ley-Hian Low; Adam Naguib; Alison Macintyre; Choo-Peng Goh; Jennifer K Callaway; Vicki Hammond; Tim Thomas; Matthew Dixon; Ulrich Putz; John Silke; Perry Bartlett; Baoli Yang; Sharad Kumar; Lloyd C Trotman; Seong-Seng Tan Journal: J Cell Biol Date: 2012-01-02 Impact factor: 10.539
Authors: Ahmed B Alarabi; Attayeb Mohsen; Kenji Mizuguchi; Fatima Z Alshbool; Fadi T Khasawneh Journal: BMC Med Genomics Date: 2022-04-14 Impact factor: 3.622