Tarek Kamal Abouzed1, Kadry M Sadek2, Emad Waded Ghazy3, Walied Abdo4, Mohmed Atef Kassab5, Salma Hago6, Samia Abdel-Wahab7, Engy A Mahrous7, Essam Abdel-Sattar7, Doaa H Assar3. 1. Department of Biochemistry, Faculty of Veterinary Medicine, Kafr-Elsheikh University, Kafr-Elsheikh, Egypt. 2. Department of Biochemistry, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt. 3. Department of Clinical Pathology, Faculty of Veterinary Medicine, Kafr-Elsheikh University, Kafr-Elsheikh, Egypt. 4. Department of Pathology, Faculty of Veterinary Medicine, Kafr-Elsheikh University, Kafr-Elsheikh, Egypt. 5. Department of Cytology and Histology, Faculty of Veterinary Medicine, Kafr-Elsheikh University, Kafr-Elsheikh, Egypt. 6. Department of Pharmacognosy, Faculty of Pharmacy, Gezira University, Wad Medani City, Sudan. 7. Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Abstract
OBJECTIVES: This study was designed to investigate the effect of Morus nigra fruit extract in retarding the progression of diabetic nephropathy in streptozotocin (STZ)-induced diabetic rats. METHODS: Diabetic male Wistar rats were injected with black mulberry fruit extract (BMFE) at doses of 150 and 300 mg/kg body weight. After 4 weeks, microalbuminuria was estimated in addition to serum concentrations of glucose, insulin, creatinine and albumin. KEY FINDINGS: The study revealed a significant amelioration of all the measured parameters in diabetic animals. In addition, MDA, lipid peroxide levels and catalase activity were also improved. The histopathological examination of kidney tissues revealed significant improvement of the pathological changes and glomerular sclerosis in diabetic rats treated with BMFE. Treated rats showed downregulation of TNF-α, vascular cell adhesion molecule-1 (VCAM-1) and fibronectin mRNA expression. CONCLUSION: The ameliorative effect of BMFE on diabetic nephropathy is not only through its potent antioxidant and hypoglycaemic effects but also through its downregulation of TNF-α, VCAM-1 and fibronectin mRNA expression in renal tissues of diabetic-treated rats. Therefore, BMFE as dietary supplement could be a promising agent in improving diabetic nephropathy.
OBJECTIVES: This study was designed to investigate the effect of Morus nigra fruit extract in retarding the progression of diabetic nephropathy in streptozotocin (STZ)-induced diabeticrats. METHODS:Diabetic male Wistar rats were injected with black mulberry fruit extract (BMFE) at doses of 150 and 300 mg/kg body weight. After 4 weeks, microalbuminuria was estimated in addition to serum concentrations of glucose, insulin, creatinine and albumin. KEY FINDINGS: The study revealed a significant amelioration of all the measured parameters in diabetic animals. In addition, MDA, lipidperoxide levels and catalase activity were also improved. The histopathological examination of kidney tissues revealed significant improvement of the pathological changes and glomerular sclerosis in diabeticrats treated with BMFE. Treated rats showed downregulation of TNF-α, vascular cell adhesion molecule-1 (VCAM-1) and fibronectin mRNA expression. CONCLUSION: The ameliorative effect of BMFE on diabetic nephropathy is not only through its potent antioxidant and hypoglycaemic effects but also through its downregulation of TNF-α, VCAM-1 and fibronectin mRNA expression in renal tissues of diabetic-treated rats. Therefore, BMFE as dietary supplement could be a promising agent in improving diabetic nephropathy.
Authors: Giftania Wardani; Jusak Nugraha; Mohd Rais Mustafa; Sri Agus Sudjarwo Journal: Evid Based Complement Alternat Med Date: 2022-05-31 Impact factor: 2.650