Shuhua Wang1, Fengxia Liu2, Hui Ma3, Xueling Cui3, Shenghua Yang4, Rui Qin5. 1. Department of Clinical Laboratory, Heze Municipal Hospital, Heze, Shandong, China. 2. Department of Clinical Laboratory, Juye Hospital of Traditional Chinese Medicine, Juye, Shandong, China. 3. Department of Breast Surgery, Heze Municipal Hospital, Heze, Shandong, China. 4. Department of Clinical Laboratory, Heze Municipal Hospital, Heze, Shandong, China. Electronic address: hsli_2019@qq.com. 5. Department of Clinical Laboratory, Heze Municipal Hospital, Heze, Shandong, China. Electronic address: qinrui8998@163.com.
Abstract
BACKGROUND: Breast cancer is the most commonly diagnosed cancer and the leading cause of cancer death among females. Circular RNAs (circRNAs) have been implicated in the initiation and development of cancer. Here, we explored the biological role and regulatory mechanism of circCDYL in breast cancer. MATERIALS AND METHODS: The expression and correlation of circCDYL/miR-190a-3p/TP53INP1 axis in breast cancer tissues and cells were determined by quantitative polymerase chain reaction and Western blot. Cell-counting Kit-8, colony formation, cell migration, and invasion assays were applied to investigate the biological roles of circCDYL in breast cancer development and progression. RESULTS: CircCDYL were down-regulated in breast cancer tissues and cells, the expression of which positively correlated with patients' survival rate. CircCDYL worked as a "sponge," binding to miR-190a-3p directly, which inhibited the expression of miR-190a-3p and relieved the inhibition of tumor suppressor gene TP53INP1. CONCLUSION: CircCDYL promotes apoptosis and inhibits proliferation of the malignant phenotype of breast cancer through regulating miR-190a-3p/TP53INP1 axis, which suggests that circCDYL is a potential therapeutic target for breast cancer.
BACKGROUND:Breast cancer is the most commonly diagnosed cancer and the leading cause of cancer death among females. Circular RNAs (circRNAs) have been implicated in the initiation and development of cancer. Here, we explored the biological role and regulatory mechanism of circCDYL in breast cancer. MATERIALS AND METHODS: The expression and correlation of circCDYL/miR-190a-3p/TP53INP1 axis in breast cancer tissues and cells were determined by quantitative polymerase chain reaction and Western blot. Cell-counting Kit-8, colony formation, cell migration, and invasion assays were applied to investigate the biological roles of circCDYL in breast cancer development and progression. RESULTS:CircCDYL were down-regulated in breast cancer tissues and cells, the expression of which positively correlated with patients' survival rate. CircCDYL worked as a "sponge," binding to miR-190a-3p directly, which inhibited the expression of miR-190a-3p and relieved the inhibition of tumor suppressor gene TP53INP1. CONCLUSION:CircCDYL promotes apoptosis and inhibits proliferation of the malignant phenotype of breast cancer through regulating miR-190a-3p/TP53INP1 axis, which suggests that circCDYL is a potential therapeutic target for breast cancer.
Authors: Brianne M Cruickshank; Marie-Claire D Wasson; Justin M Brown; Wasundara Fernando; Jaganathan Venkatesh; Olivia L Walker; Fiorella Morales-Quintanilla; Margaret L Dahn; Dejan Vidovic; Cheryl A Dean; Carter VanIderstine; Graham Dellaire; Paola Marcato Journal: Cancers (Basel) Date: 2021-05-27 Impact factor: 6.639