Elizabeth S Miller1, Camille G Apple2, Kolenkode B Kannan3, Zackary M Funk4, Philip A Efron5, Alicia M Mohr6. 1. University of Florida Health, Department of Surgery and Sepsis and Critical Illness Research Center, Gainesville, FL, USA. Electronic address: Elizabeth.Miller@surgery.ufl.edu. 2. University of Florida Health, Department of Surgery and Sepsis and Critical Illness Research Center, Gainesville, FL, USA. Electronic address: camille.apple@surgery.ufl.edu. 3. University of Florida Health, Department of Surgery and Sepsis and Critical Illness Research Center, Gainesville, FL, USA. Electronic address: Kolenkode.Kannan@surgery.ufl.edu. 4. University of Florida, College of Medicine, Gainesville, FL, USA. Electronic address: zfunk123@ufl.edu. 5. University of Florida Health, Department of Surgery and Sepsis and Critical Illness Research Center, Gainesville, FL, USA. Electronic address: Philip.Efron@surgery.ufl.edu. 6. University of Florida Health, Department of Surgery and Sepsis and Critical Illness Research Center, Gainesville, FL, USA. Electronic address: alicia.mohr@surgery.ufl.edu.
Abstract
INTRODUCTION: Propranolol has been shown to improve erythroid progenitor cell growth and anemia following trauma and this study sought to investigate the mechanisms involved by evaluating the effects of selective beta blockade. METHODS: Male Sprague-Dawley rats were subjected to lung contusion, hemorrhagic shock and chronic stress (LCHS/CS) ± daily selective beta-1, beta-2, or beta-3 blockade (B1B, B2B, B3B). Bone marrow cellularity and growth of erythroid progenitor colonies, hemoglobin, plasma granulocyte colony-stimulating factor (G-CSF), hematopoietic progenitor cell mobilization, and daily weight were assessed. RESULTS: Selective beta-2 and beta-3 blockade improved bone marrow cellularity, erythroid progenitor colony growth and hemoglobin levels, while decreasing plasma G-CSF, progenitor cell mobilization and weight loss following LCHS/CS. CONCLUSIONS: Attenuating the neuroendocrine stress response with the use of selective beta-2 and 3 adrenergic blockade may be an alternative to improve bone marrow erythroid function following trauma.
INTRODUCTION:Propranolol has been shown to improve erythroid progenitor cell growth and anemia following trauma and this study sought to investigate the mechanisms involved by evaluating the effects of selective beta blockade. METHODS: Male Sprague-Dawley rats were subjected to lung contusion, hemorrhagic shock and chronic stress (LCHS/CS) ± daily selective beta-1, beta-2, or beta-3 blockade (B1B, B2B, B3B). Bone marrow cellularity and growth of erythroid progenitor colonies, hemoglobin, plasma granulocyte colony-stimulating factor (G-CSF), hematopoietic progenitor cell mobilization, and daily weight were assessed. RESULTS: Selective beta-2 and beta-3 blockade improved bone marrow cellularity, erythroid progenitor colony growth and hemoglobin levels, while decreasing plasma G-CSF, progenitor cell mobilization and weight loss following LCHS/CS. CONCLUSIONS: Attenuating the neuroendocrine stress response with the use of selective beta-2 and 3 adrenergic blockade may be an alternative to improve bone marrow erythroid function following trauma.
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