Naoharu Morimoto1,2,3, Shu Hashimoto4, Masaya Yamanaka1, Tatsuya Nakano1, Manabu Satoh1, Yoshiharu Nakaoka1, Hisataka Iwata5, Atsushi Fukui3, Yoshiharu Morimoto6, Hiroaki Shibahara3. 1. IVF Namba Clinic, Osaka, 550-0015, Japan. 2. Reproductive Science, Graduate School of Medicine, Osaka City University, Osaka, 545-8585, Japan. 3. Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Hyogo, 663-8501, Japan. 4. Reproductive Science, Graduate School of Medicine, Osaka City University, Osaka, 545-8585, Japan. hashimoto@ivfnamba.com. 5. Department of Animal Science, Tokyo University of Agriculture, Kanagawa, 243-0034, Japan. 6. HORAC Grand Front Osaka Clinic, Osaka, 530-0011, Japan.
Abstract
PURPOSE: The fertility of women decreases with age because of factors such as an increased incidence of aneuploidies and-possibly-decreased mitochondrial activity in oocytes. However, the relationship between maternal aging and mitochondrial function of their embryos remains unknown. Here, we assessed the relationship between maternal age and mitochondrial functions in their oocytes and embryos METHODS: The relationships between maternal age and oxygen consumption rates (OCRs), mitochondrial DNA (mtDNA) copy numbers, or blastocyst development was investigated using 81 embryos donated from 63 infertility couples. The developmental rates from morulae to blastocysts were retrospectively analyzed using data of 105 patients. RESULTS: The OCRs of morulae decreased with maternal age (r2 = 0.48, P < 0.05) although there were no relationships between maternal age and mtDNA copy number in any stages. The more oxygen consumed at the morula stage, the shorter time was required for embryo development to the mid-stage blastocyst (r2 = 0.236, P < 0.05). According to the clinical data analysis, the developmental rate from morulae to blastocysts decreased with maternal age (P < 0.05, < 37 years, 81.1%, vs. ≥ 37 years, 64.1%). CONCLUSIONS: The data of the present study revealed that mitochondrial function at the morula stage of human embryos decreased with their maternal age and a decrease of mitochondrial function led to slow-paced development and impaired developmental rate from morulae to blastocysts.
PURPOSE: The fertility of women decreases with age because of factors such as an increased incidence of aneuploidies and-possibly-decreased mitochondrial activity in oocytes. However, the relationship between maternal aging and mitochondrial function of their embryos remains unknown. Here, we assessed the relationship between maternal age and mitochondrial functions in their oocytes and embryos METHODS: The relationships between maternal age and oxygen consumption rates (OCRs), mitochondrial DNA (mtDNA) copy numbers, or blastocyst development was investigated using 81 embryos donated from 63 infertility couples. The developmental rates from morulae to blastocysts were retrospectively analyzed using data of 105 patients. RESULTS: The OCRs of morulae decreased with maternal age (r2 = 0.48, P < 0.05) although there were no relationships between maternal age and mtDNA copy number in any stages. The more oxygen consumed at the morula stage, the shorter time was required for embryo development to the mid-stage blastocyst (r2 = 0.236, P < 0.05). According to the clinical data analysis, the developmental rate from morulae to blastocysts decreased with maternal age (P < 0.05, < 37 years, 81.1%, vs. ≥ 37 years, 64.1%). CONCLUSIONS: The data of the present study revealed that mitochondrial function at the morula stage of human embryos decreased with their maternal age and a decrease of mitochondrial function led to slow-paced development and impaired developmental rate from morulae to blastocysts.
Authors: T Maezawa; M Yamanaka; S Hashimoto; A Amo; A Ohgaki; Y Nakaoka; A Fukuda; T Ikeda; M Inoue; Y Morimoto Journal: J Assist Reprod Genet Date: 2014-05-25 Impact factor: 3.412
Authors: Giovana D Catandi; Yusra M Obeidat; Corey D Broeckling; Thomas W Chen; Adam J Chicco; Elaine M Carnevale Journal: Reproduction Date: 2021-04 Impact factor: 3.906
Authors: Martina Placidi; Giovanna Di Emidio; Ashraf Virmani; Angela D'Alfonso; Paolo Giovanni Artini; Anna Maria D'Alessandro; Carla Tatone Journal: Antioxidants (Basel) Date: 2022-04-08