D Warnecke1, J Balko2, J Haas3, R Bieger4, F Leucht5, N Wolf6, N B Schild7, S E C Stein8, A M Seitz9, A Ignatius10, H Reichel11, B Mizaikoff12, L Dürselen13. 1. Institute of Orthopaedic Research and Biomechanics, Centre for Trauma Research Ulm, Ulm University Medical Centre, Germany. Electronic address: daniela.warnecke@uni-ulm.de. 2. Institute of Orthopaedic Research and Biomechanics, Centre for Trauma Research Ulm, Ulm University Medical Centre, Germany. Electronic address: jonas.balko@uni-ulm.de. 3. Institute for Analytical and Bioanalytical Chemistry, Ulm University, Germany. Electronic address: julian.haas@uni-ulm.de. 4. Department of Orthopaedic Surgery, Ulm University Medical Centre, Germany. Electronic address: ralf.bieger@uni-ulm.de. 5. Department of Orthopaedic Surgery, Ulm University Medical Centre, Germany. Electronic address: frank.leucht@rku.de. 6. Institute of Orthopaedic Research and Biomechanics, Centre for Trauma Research Ulm, Ulm University Medical Centre, Germany. Electronic address: wolf.nicolas@gmx.de. 7. Institute of Orthopaedic Research and Biomechanics, Centre for Trauma Research Ulm, Ulm University Medical Centre, Germany. Electronic address: natalie.b.schild@gmail.com. 8. Institute of Orthopaedic Research and Biomechanics, Centre for Trauma Research Ulm, Ulm University Medical Centre, Germany. Electronic address: svenja.stein@uni-ulm.de. 9. Institute of Orthopaedic Research and Biomechanics, Centre for Trauma Research Ulm, Ulm University Medical Centre, Germany. Electronic address: andreas.seitz@uni-ulm.de. 10. Institute of Orthopaedic Research and Biomechanics, Centre for Trauma Research Ulm, Ulm University Medical Centre, Germany. Electronic address: anita.ignatius@uni-ulm.de. 11. Department of Orthopaedic Surgery, Ulm University Medical Centre, Germany. Electronic address: heiko.reichel@uni-ulm.de. 12. Institute for Analytical and Bioanalytical Chemistry, Ulm University, Germany. Electronic address: boris.mizaikoff@uni-ulm.de. 13. Institute of Orthopaedic Research and Biomechanics, Centre for Trauma Research Ulm, Ulm University Medical Centre, Germany. Electronic address: lutz.duerselen@uni-ulm.de.
Abstract
OBJECTIVE: Because the literature relating to the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci remains contradictory or completely lacking, the aim of this study was to fill these gaps by comprehensively characterising the biomechanical properties of menisci with regard to the degree of degeneration. DESIGN: Meniscal tissue from 24 patients undergoing a total knee replacement was collected and the degeneration of each region classified according to Pauli et al. For biomechanical characterisation, compression and tensile tests were performed. Additionally, the water content was determined and infrared (IR) spectroscopy was applied to detect changes in the structural composition, particularly of the proteoglycan and collagen content. RESULTS: With an increasing degree of degeneration, a significant decrease of the equilibrium modulus was detected, while simultaneously the water content and the hydraulic permeability significantly increased. However, the tensile modulus displayed a tendency to decrease with increasing degeneration, which might be due to the significantly decreasing amount of collagen content identified by the IR measurements. CONCLUSION: The findings of the current study may contribute to the understanding of meniscus degeneration, showing that degenerative processes appear to mainly worsen viscoelastic properties of the inner circumference by disrupting the collagen integrity.
OBJECTIVE: Because the literature relating to the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci remains contradictory or completely lacking, the aim of this study was to fill these gaps by comprehensively characterising the biomechanical properties of menisci with regard to the degree of degeneration. DESIGN: Meniscal tissue from 24 patients undergoing a total knee replacement was collected and the degeneration of each region classified according to Pauli et al. For biomechanical characterisation, compression and tensile tests were performed. Additionally, the water content was determined and infrared (IR) spectroscopy was applied to detect changes in the structural composition, particularly of the proteoglycan and collagen content. RESULTS: With an increasing degree of degeneration, a significant decrease of the equilibrium modulus was detected, while simultaneously the water content and the hydraulic permeability significantly increased. However, the tensile modulus displayed a tendency to decrease with increasing degeneration, which might be due to the significantly decreasing amount of collagen content identified by the IR measurements. CONCLUSION: The findings of the current study may contribute to the understanding of meniscus degeneration, showing that degenerative processes appear to mainly worsen viscoelastic properties of the inner circumference by disrupting the collagen integrity.
Authors: Andy Morejon; Christopher D Norberg; Massimiliano De Rosa; Thomas M Best; Alicia R Jackson; Francesco Travascio Journal: Front Bioeng Biotechnol Date: 2021-02-10