OBJECTIVE: The aim of this study was to examine the effects of sex and menopausal status on depot-specific estrogen signaling in white adipose tissue (AT) in age-matched men and women with morbid obesity. METHODS: A total of 28 premenopausal women, 16 postmenopausal women, and 27 age-matched men undergoing bariatric surgery were compared for omental (OM) AT (OMAT) and abdominal subcutaneous (SQ) AT (SQAT) genes and proteins. RESULTS: With the exception of fasting nonesterified fatty acids being higher in women (P < 0.01), no differences were found in other indicators of glucose and lipid metabolism. In OMAT, estrogen receptor (ER) beta (ERβ) levels were higher in older women than in younger women and older men (sex-age interaction, P < 0.01), and aromatase expression was higher in older men than in older women (P < 0.05). In SQAT, women had lower expression of ERβ than men (P < 0.05). Protein content of ER alpha and ERβ was highly correlated with the mitochondrial protein uncoupling protein 1 across sexes and ages (P < 0.001). Age increased SQ inflammatory gene expression in both sexes. CONCLUSIONS: In morbid obesity, sex and age affect AT ERs, lipid metabolism, mitochondrial uncoupling protein 1, and inflammatory expression in an AT depot-dependent manner. The SQAT immunometabolic profile is heavily influenced by age and menopause status, more so than OMAT.
OBJECTIVE: The aim of this study was to examine the effects of sex and menopausal status on depot-specific estrogen signaling in white adipose tissue (AT) in age-matched men and women with morbid obesity. METHODS: A total of 28 premenopausal women, 16 postmenopausal women, and 27 age-matched men undergoing bariatric surgery were compared for omental (OM) AT (OMAT) and abdominal subcutaneous (SQ) AT (SQAT) genes and proteins. RESULTS: With the exception of fasting nonesterified fatty acids being higher in women (P < 0.01), no differences were found in other indicators of glucose and lipid metabolism. In OMAT, estrogen receptor (ER) beta (ERβ) levels were higher in older women than in younger women and older men (sex-age interaction, P < 0.01), and aromatase expression was higher in older men than in older women (P < 0.05). In SQAT, women had lower expression of ERβ than men (P < 0.05). Protein content of ER alpha and ERβ was highly correlated with the mitochondrial protein uncoupling protein 1 across sexes and ages (P < 0.001). Age increased SQ inflammatory gene expression in both sexes. CONCLUSIONS: In morbid obesity, sex and age affect AT ERs, lipid metabolism, mitochondrial uncoupling protein 1, and inflammatory expression in an AT depot-dependent manner. The SQAT immunometabolic profile is heavily influenced by age and menopause status, more so than OMAT.
Authors: Nathan C Winn; Thomas J Jurrissen; Zachary I Grunewald; Rory P Cunningham; Makenzie L Woodford; Jill A Kanaley; Dennis B Lubahn; Camila Manrique-Acevedo; R Scott Rector; Victoria J Vieira-Potter; Jaume Padilla Journal: Am J Physiol Endocrinol Metab Date: 2018-12-04 Impact factor: 4.310
Authors: Susan M Majka; Keith E Fox; John C Psilas; Karen M Helm; Christine R Childs; Alistaire S Acosta; Rachel C Janssen; Jacob E Friedman; Brian T Woessner; Theodore R Shade; Marileila Varella-Garcia; Dwight J Klemm Journal: Proc Natl Acad Sci U S A Date: 2010-08-02 Impact factor: 11.205
Authors: Neil M Iyengar; Kristy A Brown; Xi Kathy Zhou; Ayca Gucalp; Kotha Subbaramaiah; Dilip D Giri; Heba Zahid; Priya Bhardwaj; Nils K Wendel; Domenick J Falcone; Hanhan Wang; Samantha Williams; Michael Pollak; Monica Morrow; Clifford A Hudis; Andrew J Dannenberg Journal: Cancer Prev Res (Phila) Date: 2017-03-07
Authors: Natalia Hetemäki; Hanna Savolainen-Peltonen; Matti J Tikkanen; Feng Wang; Hanna Paatela; Esa Hämäläinen; Ursula Turpeinen; Mikko Haanpää; Veera Vihma; Tomi S Mikkola Journal: J Clin Endocrinol Metab Date: 2017-12-01 Impact factor: 5.958
Authors: Laura M Clart; Rebecca J Welly; Eric D Queathem; R Scott Rector; Jaume Padilla; Christopher P Baines; Jill A Kanaley; Dennis B Lubahn; Victoria J Vieira-Potter Journal: J Endocrinol Date: 2021-05-24 Impact factor: 4.669
Authors: Eric D Queathem; Maggie Fitzgerald; Rebecca Welly; Candace C Rowles; Kylie Schaller; Shahad Bukhary; Christopher P Baines; R Scott Rector; Jaume Padilla; Camila Manrique-Acevedo; Dennis B Lubahn; Victoria J Vieira-Potter Journal: Front Physiol Date: 2022-09-16 Impact factor: 4.755
Authors: Eric D Queathem; Rebecca J Welly; Laura M Clart; Candace C Rowles; Hunter Timmons; Maggie Fitzgerald; Peggy A Eichen; Dennis B Lubahn; Victoria J Vieira-Potter Journal: Cells Date: 2021-12-08 Impact factor: 6.600