Literature DB >> 3273408

Optimization of ligation reaction conditions in gene synthesis.

N Y Theriault1, J B Carter, S P Pulaski.   

Abstract

Several phosphorylation, annealing and ligation reaction conditions have been investigated for the enzymatic assembly of genes of interest. The use of longer oligodeoxyribonucleotides (40-60 bases long) also improved the enzymatic reaction. Furthermore, the use of longer oligonucleotides and the elimination of long stretches of G's or C's lowered the mutation rate.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3273408

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  5 in total

1.  Expression of fully functional tetrameric human hemoglobin in Escherichia coli.

Authors:  S J Hoffman; D L Looker; J M Roehrich; P E Cozart; S L Durfee; J L Tedesco; G L Stetler
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

2.  Pseudouridine modification of U5 RNA in ribonucleoprotein particles assembled in vitro.

Authors:  J R Patton
Journal:  Mol Cell Biol       Date:  1991-12       Impact factor: 4.272

3.  Computationally Optimised DNA Assembly of synthetic genes.

Authors:  Liza S Z Larsen; Christopher D Wassman; G Wesley Hatfield; Richard H Lathrop
Journal:  Int J Bioinform Res Appl       Date:  2008

4.  Identification of essential elements in U14 RNA of Saccharomyces cerevisiae.

Authors:  A Jarmolowski; J Zagorski; H V Li; M J Fournier
Journal:  EMBO J       Date:  1990-12       Impact factor: 11.598

5.  U14 function in Saccharomyces cerevisiae can be provided by large deletion variants of yeast U14 and hybrid mouse-yeast U14 RNAs.

Authors:  D Li; M J Fournier
Journal:  EMBO J       Date:  1992-02       Impact factor: 11.598
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.