R Limão1,2, A Spínola Santos1,2, L Araújo2,3, J Cosme1,2, F Inácio2,4, E Tomaz4, A Ferrão5, N Santos6, A Sokolova2,7, A Môrete8, H Falcão9, L Cunha9, A Ferreira10, A Bras11, F Ribeiro11, C Lozoya12, P Leiria Pinto13, S Prates13, J Plácido14, A Coimbra14, L Taborda-Barata15, M C Pereira Santos2,16, M Pereira Barbosa1,16,17, F Pineda18. 1. Immunoallergology Department, Hospital de Santa Maria, Centro Hospitalar Universitário Lisboa Norte, Portugal. 2. Allergen and Immunotherapy Interest Group, Sociedade Portuguesa de Alergologia e Imunologia Clínica, Portugal. 3. Immunoallergology Department, Faculdade de Medicina da Universidade do Porto, Portugal. 4. Immunoallergology Department, Hospital de São Bernardo, Setúbal, Portugal. 5. Immunoallergology Unit, Hospital do Espírito Santo de Évora, Portugal. 6. Immunoallergology Unit, Hospital de Portimão, Centro Hospitalar Universitário do Algarve, Portugal. 7. Immunoallergology Unit, Hospital Professor Doutor Fernando Fonseca, Amadora-Sintra, Portugal. 8. Immunoallergology Unit, Hospital de Aveiro, Centro Hospitalar Baixo Vouga, Portugal. 9. Immunoallergology Department, Hospital de Santo António, Centro Hospitalar do Porto, Portugal. 10. Immunoallergology Unit, Hospital das Forças Armadas, Lisboa, Portugal. 11. Immunoallergology Unit, Hospital de Faro, Centro Hospitalar Universitário do Algarve, Portugal. 12. Immunoallergology Unit, Unidade Local de Saúde de Castelo Branco, Portugal. 13. Immunoallergology Department, Hospital Dona Estefânia, Centro Hospitalar Universitário de Lisboa Central, Portugal. 14. Immunoallergology Department, Centro Hospitalar Universitário de São João, Porto, Portugal. 15. Immunoallergology Department, Centro Hospitalar Universitário Cova da Beira, Covilhã, Portugal. 16. Clinical Immunology Laboratory, Faculdade de Medicina, Instituto de Medicina Molecular, Uni-versidade de Lisboa, Portugal. 17. University Clinic of Immunoallergology, Faculdade de Medicina da Universidade de Lisboa, Por-tugal. 18. Diater Laboratorio de Diagnostico y Aplicaciones Terapeuticas SA, Madrid, Spain.
Abstract
BACKGROUND AND OBJECTIVES: To analyze component-resolved diagnosis of sensitization to Dermatophagoides pteronyssinus (Der p) in patients with respiratory allergy and the association between diagnostic findings and clinical severity in different geographical areas. METHODS: The study population comprised 217 patients (mean age, 25.85 [12.7] years; 51.16% female) selected from 13 centers in Portugal (5 from the North, n=65). All had allergic rhinitis with or without asthma and positive skin prick test results to at least 1 dust mite. Specific IgE (sIgE) to Der p, Dermatophagoides farinae, Lepidoglyphus destructor, Der p 1, Der p 2, Der p 10, and Der p 23 was determined using ImmunoCAP. The Mann-Whitney test was applied for the following comparisons: rhinitis vs rhinitis and asthma; mild vs moderate-to-severe rhinitis; North vs South. RESULTS: The prevalence of sensitization was 98.2% for Der p, and 72.4%, 89.4%, 9.7%, and 77% for Der p 1, Der p 2, Der p 10, and Der p 23, respectively. The corresponding median sIgE levels were 8.56, 17.7, 0.01, and 3.95 kUA/L. sIgE to all allergens was higher in patients with moderate-to-severe rhinitis and rhinitis with asthma (nonsignficant). Concentrations of sIgE to Der p 2 were significantly higher in the South than in the North (P=.0496). CONCLUSION: The most common sensitization in Portugal was to Der p. The highest prevalence and median sIgE level were observed for Der p 2. All sIgE values for molecular components were higher in more symptomatic patients (nonsignificant). Concentrations of sIgE to Der p 2 were higher in the South, probably because of the warmer temperature and/or the larger sample size.
BACKGROUND AND OBJECTIVES: To analyze component-resolved diagnosis of sensitization to Dermatophagoides pteronyssinus (Der p) in patients with respiratory allergy and the association between diagnostic findings and clinical severity in different geographical areas. METHODS: The study population comprised 217 patients (mean age, 25.85 [12.7] years; 51.16% female) selected from 13 centers in Portugal (5 from the North, n=65). All had allergic rhinitis with or without asthma and positive skin prick test results to at least 1 dust mite. Specific IgE (sIgE) to Der p, Dermatophagoides farinae, Lepidoglyphus destructor, Der p 1, Der p 2, Der p 10, and Der p 23 was determined using ImmunoCAP. The Mann-Whitney test was applied for the following comparisons: rhinitis vs rhinitis and asthma; mild vs moderate-to-severe rhinitis; North vs South. RESULTS: The prevalence of sensitization was 98.2% for Der p, and 72.4%, 89.4%, 9.7%, and 77% for Der p 1, Der p 2, Der p 10, and Der p 23, respectively. The corresponding median sIgE levels were 8.56, 17.7, 0.01, and 3.95 kUA/L. sIgE to all allergens was higher in patients with moderate-to-severe rhinitis and rhinitis with asthma (nonsignficant). Concentrations of sIgE to Der p 2 were significantly higher in the South than in the North (P=.0496). CONCLUSION: The most common sensitization in Portugal was to Der p. The highest prevalence and median sIgE level were observed for Der p 2. All sIgE values for molecular components were higher in more symptomatic patients (nonsignificant). Concentrations of sIgE to Der p 2 were higher in the South, probably because of the warmer temperature and/or the larger sample size.