Literature DB >> 327278

The use of a novel plate assay in a search for yeast mutants defective in deoxyribonucleases.

L H Johnston.   

Abstract

Methods by which the intracellular enzymes deoxyribonuclease, ribonuclease and protease can be assayed in whole colonies of Saccharomyces cerevisiae on agar plates are described. A search for mutants deficient in deoxyribonuclease has been carried out. Two types of mutant are described. One apparently fails to produce deoxyribonuclease, ribonuclease or protease on agar plates and the other apparently fails to produce deoxyribonuclease and ribonuclease.

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Year:  1977        PMID： 327278     DOI： 10.1007/bf00268821

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  23 in total

Review 1.  Enzymatic repair of DNA.

Authors:  L Grossman; A Braun; R Feldberg; I Mahler
Journal:  Annu Rev Biochem       Date:  1975       Impact factor: 23.643

Review 2.  Methods for avoiding proteolytic artefacts in studies of enzymes and other proteins from yeasts.

Authors:  J R Pringle
Journal:  Methods Cell Biol       Date:  1975       Impact factor: 1.441

3.  AN ENDONUCLEASE FROM NEUROSPORA CRASSA SPECIFIC FOR POLYNUCLEOTIDES LACKING AN ORDERED STRUCTURE. I. PURIFICATION AND PROPERTIES OF THE ENZYME.

Authors:  S LINN; I R LEHMAN
Journal:  J Biol Chem       Date:  1965-03       Impact factor: 5.157

4.  Method for decryptification of -glucosidase in yeast with dimethyl sulfoxide.

Authors:  B G Adams
Journal:  Anal Biochem       Date:  1972-01       Impact factor: 3.365

5.  An exonuclease of Neurospora crassa specific for single-stranded nucleic acids.

Authors:  E Z Rabin; H Tenenhouse; M J Fraser
Journal:  Biochim Biophys Acta       Date:  1972-01-18

6.  The nucleases of yeast. II. Purification, properties and specificity of an endonuclease from yeast.

Authors:  S Y Lee; Y Nakao; R M Bock
Journal:  Biochim Biophys Acta       Date:  1968-01-08

7.  Characterization of a yeast endonuclease.

Authors:  R Piñon
Journal:  Biochemistry       Date:  1970-07-07       Impact factor: 3.162

8.  Studies on deoxyribonucleases from Saccharomyces cerevisiae. Characterization of two endonuclease activities with a preference for double-stranded DNA.

Authors:  R Pinon; E Leney
Journal:  Nucleic Acids Res       Date:  1975-07       Impact factor: 16.971

9.  Purification and properties of the nuclease inhibitor of Aspergillus oryzae and kinetics of its interaction with crystalline nuclease O.

Authors:  T Uozumi; K Ishino; T Beppu; K Arima
Journal:  J Biol Chem       Date:  1976-05-10       Impact factor: 5.157

10.  Host-controlled restriction of T-even bacteriophages: relation of four bacterial deoxyribonucleases to restriction.

Authors:  J Eigner; S Block
Journal:  J Virol       Date:  1968-04       Impact factor: 5.103
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  6 in total

1.  Nuclear mutations in Saccharomyces cerevisiae which increase the spontaneous mutation frequency in mitochondrial DNA.

Authors:  L H Johnston
Journal:  Mol Gen Genet       Date:  1979-03-05

2.  Conditional mutants of RPC160, the gene encoding the largest subunit of RNA polymerase C in Saccharomyces cerevisiae.

Authors:  R Gudenus; S Mariotte; A Moenne; A Ruet; S Memet; J M Buhler; A Sentenac; P Thuriaux
Journal:  Genetics       Date:  1988-07       Impact factor: 4.562

3.  Mutants of yeast with depressed DNA synthesis.

Authors:  L H Johnston; J C Game
Journal:  Mol Gen Genet       Date:  1978-05-03

4.  Isolation and genetic analysis of nuclease halo (nuh) mutants of Neurospora crassa.

Authors:  E Käfer; M Fraser
Journal:  Mol Gen Genet       Date:  1979-01-31

5.  Regulation of glutamine-repressible gene products by the GLN3 function in Saccharomyces cerevisiae.

Authors:  A P Mitchell; B Magasanik
Journal:  Mol Cell Biol       Date:  1984-12       Impact factor: 4.272

6.  An endo-exonuclease activity of yeast that requires a functional RAD52 gene.

Authors:  T Y Chow; M A Resnick
Journal:  Mol Gen Genet       Date:  1988-01
  6 in total

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