Prevalence, phylogenomic insights, and phenotypic characterization of Salmonella enterica isolated from meats in the Tamale metropolis of Ghana.

Characterization of foodborne pathogens including Salmonella species allows for the determination of their relationship and/or relatedness with others. This study characterized Salmonella enterica (S. enterica) isolated from five meat types (mutton, beef, chevon, guinea fowl, and local chicken) obtained from Tamale metropolis, Ghana. The S. enterica were characterized phenotypically (n = 44) based on their antibiotic resistance pattern with the disc diffusion method and genetically (n = 16) using whole-genome sequencing (WGS) as well as with bioinformatic analysis for the prediction of their clonal and phylogenomic relationship. Of the 225 meat samples examined, 107 (47.56%) were positive for S. enterica. Mutton was the most contaminated meat type and the least was local chicken. The 44 S. enterica isolates exhibited five different antibiotic patterns with multiple antibiotic resistance (MAR) index ranging from 0.13 to 0.63. Resistant to only erythromycin was most common and was exhibited by 34 isolates (77.27%). Four isolates were resistant to four different antibiotics (TeAmpSxtECro) with a percentage of 9.09%, while two isolates (4.55%) were resistant to none of the antibiotics. The sequenced S. enterica isolates consisted of 7 serovars and 8 clonal lineages with the S. enterica subsp. enterica serovar Hato (ST5308) being the predominate strain. Phylogenomic analysis showed that the isolates clustered according to their serovars and sequence types (clonal lineages). However, further metadata insights coupled with the phylogenomics revealed a complex intraspread of multiple S. enterica subsp. enterica serovars in diverse meat sources in areas in Tamale which is very worrying for infection management. In summary, our study provides useful insights into S. enterica in meat reservoirs obtained from Tamale metropolis, Ghana.