Sunil Kumar Bose1, Pradip Nirbhavane2, Mahak Batra3, Sanjay Chhibber1, Kusum Harjai1. 1. Department of Microbiology, BMS Block-I, Panjab University, Chandigarh, 160014, India. 2. University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, 160014, India. 3. Centre for Systems Biology & Bioinformatics, Panjab University, Chandigarh, 160014, India.
Abstract
Aim: Pseudomonas aeruginosa has emerged as a major opportunistic pathogen meaning there is an immediate need to develop efficient antivirulence agents which offer a new class of superior therapeutics. Methods: Nanostructured lipid carriers (NLCs) containing α-terpineol (αT) were developed and characterized to determine expression profiles of quorum sensing regulated genes, antivirulence activity and antibiofilm effects against P. aeruginosa. Results: The αT-NLCs had a size of 145.4 nm, polydispersity index of 0.242 and ζ-potential of -31.4 mV. They exhibited pronounced effects on the inhibition of quorum sensing mediated virulence and biofilm formation which were confirmed by molecular docking analysis and gene expression profiles. Conclusion: αT-NLCs show promise as effective antivirulence agents against P. aeruginosa in the postantibiotic era.
Aim: Pseudomonas aeruginosa has emerged as a major opportunistic pathogen meaning there is an immediate need to develop efficient antivirulence agents which offer a new class of superior therapeutics. Methods: Nanostructured lipid carriers (NLCs) containing α-terpineol (αT) were developed and characterized to determine expression profiles of quorum sensing regulated genes, antivirulence activity and antibiofilm effects against P. aeruginosa. Results: The αT-NLCs had a size of 145.4 nm, polydispersity index of 0.242 and ζ-potential of -31.4 mV. They exhibited pronounced effects on the inhibition of quorum sensing mediated virulence and biofilm formation which were confirmed by molecular docking analysis and gene expression profiles. Conclusion: αT-NLCs show promise as effective antivirulence agents against P. aeruginosa in the postantibiotic era.
Entities:
Keywords:
Pseudomonas aeruginosa; antivirulence; biofilms; nanoencapsulation; quorum sensing; α-terpineol
Authors: Ariadna Thalia Bernal-Mercado; Josué Juarez; Miguel Angel Valdez; Jesus Fernando Ayala-Zavala; Carmen Lizette Del-Toro-Sánchez; David Encinas-Basurto Journal: Molecules Date: 2022-01-21 Impact factor: 4.411