Literature DB >> 3272179

A cell type-specific enhancer drives expression of the chick muscle acetylcholine receptor alpha-subunit gene.

Y Wang1, H P Xu, X M Wang, M Ballivet, J Schmidt.   

Abstract

The regulation of acetylcholine receptor alpha-subunit gene expression was analyzed by transient expression assays. Using rabbit beta-globin cDNA as a reporter gene, we have confirmed that the 5'-flanking sequence of the chicken acetylcholine receptor alpha-subunit gene directs specific expression in differentiated C2C12 cells, a mouse muscle cell line, but not in undifferentiated C2C12 cells and mouse 3T3 fibroblasts. Testing chimeric plasmids containing Bal31 deletion mutants of the alpha-subunit gene upstream sequence, we found the -116 to -81 region of the alpha-subunit to be responsible for tissue- and stage-specific expression. This 36 bp fragment stimulates the activity of both alpha-subunit and SV40 promoters in a distance- and orientation-independent manner, thus fulfilling the criteria of an enhancer.

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Year:  1988        PMID： 3272179     DOI： 10.1016/0896-6273(88)90183-3

Source DB:  PubMed          Journal:  Neuron        ISSN： 0896-6273            Impact factor:   17.173


  23 in total

1.  Analysis of binding and activating functions of the chick muscle acetylcholine receptor gamma-subunit upstream sequence.

Authors:  H T Jia; H J Tsay; J Schmidt
Journal:  Cell Mol Neurobiol       Date:  1992-06       Impact factor: 5.046

2.  The 5'-flanking region of the mouse muscle nicotinic acetylcholine receptor beta subunit gene promotes expression in cultured muscle cells and is activated by MRF4, myogenin and myoD.

Authors:  C A Prody; J P Merlie
Journal:  Nucleic Acids Res       Date:  1992-05-11       Impact factor: 16.971

3.  A highly conserved enhancer downstream of the human MLC1/3 locus is a target for multiple myogenic determination factors.

Authors:  N Rosenthal; E B Berglund; B M Wentworth; M Donoghue; B Winter; E Bober; T Braun; H H Arnold
Journal:  Nucleic Acids Res       Date:  1990-11-11       Impact factor: 16.971

4.  CTF4, a chicken transcription factor of the helix-loop-helix class A family.

Authors:  H J Tsay; Y H Choe; C M Neville; J Schmidt
Journal:  Nucleic Acids Res       Date:  1992-04-11       Impact factor: 16.971

5.  Induction of acetylcholine receptor alpha-subunit gene expression in chicken myotubes by blocking electrical activity requires ongoing protein synthesis.

Authors:  A Duclert; J Piette; J P Changeux
Journal:  Proc Natl Acad Sci U S A       Date:  1990-02       Impact factor: 11.205

6.  A new myocyte-specific enhancer-binding factor that recognizes a conserved element associated with multiple muscle-specific genes.

Authors:  L A Gossett; D J Kelvin; E A Sternberg; E N Olson
Journal:  Mol Cell Biol       Date:  1989-11       Impact factor: 4.272

Review 7.  Activity-dependent regulation of gene expression in muscle and neuronal cells.

Authors:  R Laufer; J P Changeux
Journal:  Mol Neurobiol       Date:  1989 Spring-Summer       Impact factor: 5.590

8.  An 83-nucleotide promoter of the acetylcholine receptor epsilon-subunit gene confers preferential synaptic expression in mouse muscle.

Authors:  A Duclert; N Savatier; J P Changeux
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-01       Impact factor: 11.205

9.  Paired MyoD-binding sites regulate myosin light chain gene expression.

Authors:  B M Wentworth; M Donoghue; J C Engert; E B Berglund; N Rosenthal
Journal:  Proc Natl Acad Sci U S A       Date:  1991-02-15       Impact factor: 11.205

10.  Response of myogenic determination factors to cessation and resumption of electrical activity in skeletal muscle: a possible role for myogenin in denervation supersensitivity.

Authors:  C M Neville; M Schmidt; J Schmidt
Journal:  Cell Mol Neurobiol       Date:  1992-12       Impact factor: 5.046
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