Literature DB >> 32720666

Efficient construction of a stable linear gene based on a TNA loop modified primer pair for gene delivery.

Xuehe Lu1, Xiaohui Wu2, Tiantian Wu2, Lin Han1, Jianbing Liu2, Baoquan Ding3.   

Abstract

A terminal-closed linear gene with strong exonuclease resistance and serum stability was successfully constructed by polymerase chain reaction (PCR) with an α-l-threose nucleic acid (TNA) loop modified primer pair, which can be used as an efficient gene expression system in eukaryotic cells for gene delivery.

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Year:  2020        PMID： 32720666     DOI： 10.1039/d0cc04356g

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  3 in total

1.  Cellular uptake, tissue penetration, biodistribution, and biosafety of threose nucleic acids: Assessing in vitro and in vivo delivery.

Authors:  Fei Wang; Ling Sum Liu; Pan Li; Cia Hin Lau; Hoi Man Leung; Y Rebecca Chin; Chung Tin; Pik Kwan Lo
Journal:  Mater Today Bio       Date:  2022-05-18

Review 2.  Nucleic Acids and Their Analogues for Biomedical Applications.

Authors:  Fei Wang; Pan Li; Hoi Ching Chu; Pik Kwan Lo
Journal:  Biosensors (Basel)       Date:  2022-02-04

3.  Biologically stable threose nucleic acid-based probes for real-time microRNA detection and imaging in living cells.

Authors:  Fei Wang; Ling Sum Liu; Pan Li; Hoi Man Leung; Dick Yan Tam; Pik Kwan Lo
Journal:  Mol Ther Nucleic Acids       Date:  2022-01-03       Impact factor: 8.886
  3 in total

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