Literature DB >> 32719012

A cytoskeletal protein complex is essential for division of intracellular amastigotes of Leishmania mexicana.

Felice D Kelly1, Khoa D Tran1, Jess Hatfield1, Kat Schmidt1, Marco A Sanchez1, Scott M Landfear2.   

Abstract

Previous studies in Leishmania mexicana have identified the cytoskeletal protein KHARON as being important for both flagellar trafficking of the glucose transporter GT1 and for successful cytokinesis and survival of infectious amastigote forms inside mammalian macrophages. KHARON is located in three distinct regions of the cytoskeleton: the base of the flagellum, the subpellicular microtubules, and the mitotic spindle. To deconvolve the different functions for KHARON, we have identified two partner proteins, KHAP1 and KHAP2, which associate with KHARON. KHAP1 is located only in the subpellicular microtubules, whereas KHAP2 is located at the subpellicular microtubules and the base of the flagellum. Both KHAP1 and KHAP2 null mutants are unable to execute cytokinesis but are able to traffic GT1 to the flagellum. These results confirm that KHARON assembles into distinct functional complexes and that the subpellicular complex is essential for cytokinesis and viability of disease-causing amastigotes but not for flagellar membrane trafficking.
© 2020 Kelly et al.

Entities:  

Keywords:  Leishmania; cytokinesis; cytoskeleton; flagellum; infectious disease; protein complex; protein targeting

Mesh：

Substances：

Year:  2020        PMID： 32719012      PMCID： PMC7489907          DOI： 10.1074/jbc.RA120.014065

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  49 in total

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2.  Predicting coiled coils from protein sequences.

Authors:  A Lupas; M Van Dyke; J Stock
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3.  KHARON1 mediates flagellar targeting of a glucose transporter in Leishmania mexicana and is critical for viability of infectious intracellular amastigotes.

Authors:  Khoa D Tran; Dayana Rodriguez-Contreras; Danielle P Vieira; Phillip A Yates; Larry David; Wandy Beatty; Johannes Elferich; Scott M Landfear
Journal:  J Biol Chem       Date:  2013-06-13       Impact factor: 5.157

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10.  Flagellar pocket restructuring through the Leishmania life cycle involves a discrete flagellum attachment zone.

Authors:  Richard J Wheeler; Jack D Sunter; Keith Gull
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  5 in total

Review 1.  Tag Thy Neighbour: Nanometre-Scale Insights Into Kinetoplastid Parasites With Proximity Dependent Biotinylation.

Authors:  Vincent Geoghegan; Jeremy C Mottram; Nathaniel G Jones
Journal:  Front Cell Infect Microbiol       Date:  2022-05-06       Impact factor: 6.073

Review 2.  Nutrient sensing in Leishmania: Flagellum and cytosol.

Authors:  Felice D Kelly; Phillip A Yates; Scott M Landfear
Journal:  Mol Microbiol       Date:  2020-11-21       Impact factor: 3.501

Review 3.  Mass spectrometry-based protein-protein interaction networks for the study of human diseases.

Authors:  Alicia L Richards; Manon Eckhardt; Nevan J Krogan
Journal:  Mol Syst Biol       Date:  2021-01       Impact factor: 11.429

Review 4.  New Vistas in the Biology of the Flagellum-Leishmania Parasites.

Authors:  Scott M Landfear
Journal:  Pathogens       Date:  2022-04-07

5.  The kinesin of the flagellum attachment zone in Leishmania is required for cell morphogenesis, cell division and virulence in the mammalian host.

Authors:  Rosa Milagros Corrales; Slavica Vaselek; Rachel Neish; Laurence Berry; Camille D Brunet; Lucien Crobu; Nada Kuk; Julio Mateos-Langerak; Derrick R Robinson; Petr Volf; Jeremy C Mottram; Yvon Sterkers; Patrick Bastien
Journal:  PLoS Pathog       Date:  2021-06-18       Impact factor: 6.823
  5 in total

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