Handan Derebaşınlıoğlu1, Anil Demİröz2, Yağmur Aydin2, Hakan Ekmekçi3, Özlem Balci Ekmekçi3, Övgü Aydin4, Levent Cankorkmaz5. 1. Department of Plastic Reconstructive and Aesthetic Surgery, Faculty of Medicine, Sivas Cumhuriyet University, Sivas, Turkey 2. Department of Plastic Reconstructive and Aesthetic Surgery, Faculty of Medicine, İstanbul University Cerrahpaşa Medical School, İstanbul, Turkey 3. Department of Biochemistry, Faculty of Medicine, İstanbul University Cerrahpaşa Medical School, İstanbul, Turkey 4. Department of Pathology, Faculty of Medicine, İstanbul University Cerrahpaşa Medical School, İstanbul, Turkey 5. Department of Pediatric Surgery, Faculty of Medicine, Sivas Cumhuriyet University, Sivas, Turkey
Abstract
Background/aim: The aim of the study was to evaluate the protective effect of Botulinum A toxin injection against ischemia-reperfusion injury. Materials and methods: Thirty-two Sprague-Dawley rats were divided into: control, ischemia-reperfusion, ischemic preconditioning, and botulinum groups. In all groups the musculocutaneous pedicle flap was occluded for 4 h, and then reperfused to induce ischemia-reperfusion injury. Serum and tissue myeloperoxidase (MPO) and nitric oxide (NO) levels were measured at 24 h and at 10 days. Results: Tissue MPO levels did not differ significantly between the ischemic preconditioning and botulinum groups at 24 h but was significantly lower in the botulinum group at 10 days. Tissue NO levels were significantly higher in the ischemic preconditioning group compared to the botulinum group at 24 h and at 10 days. Serum MPO showed no significant difference between these two groups at 24 h but was significantly lower in the ischemic preconditioning group compared to the botulinum group at 10 days. Serum NO levels were not significantly different at 24 h but significantly higher in the botulinum group at 10 days. Conclusion: Findings show that botulinum has a protective effect against the ischemia-reperfusion injury via increased NO and decreased MPO levels in tissue. Based on tissue NO levels, ischemic preconditioning was significantly higher than botulinum. This work is licensed under a Creative Commons Attribution 4.0 International License.
Background/aim: The aim of the study was to evaluate the protective effect of Botulinum A toxin injection against ischemia-reperfusion injury. Materials and methods: Thirty-two Sprague-Dawley rats were divided into: control, ischemia-reperfusion, ischemic preconditioning, and botulinum groups. In all groups the musculocutaneous pedicle flap was occluded for 4 h, and then reperfused to induce ischemia-reperfusion injury. Serum and tissue myeloperoxidase (MPO) and nitric oxide (NO) levels were measured at 24 h and at 10 days. Results: Tissue MPO levels did not differ significantly between the ischemic preconditioning and botulinum groups at 24 h but was significantly lower in the botulinum group at 10 days. Tissue NO levels were significantly higher in the ischemic preconditioning group compared to the botulinum group at 24 h and at 10 days. Serum MPO showed no significant difference between these two groups at 24 h but was significantly lower in the ischemic preconditioning group compared to the botulinum group at 10 days. Serum NO levels were not significantly different at 24 h but significantly higher in the botulinum group at 10 days. Conclusion: Findings show that botulinum has a protective effect against the ischemia-reperfusion injury via increased NO and decreased MPO levels in tissue. Based on tissue NO levels, ischemic preconditioning was significantly higher than botulinum. This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors: Peter B Arnold; Wyndell Merritt; George T Rodeheaver; Chris A Campbell; Raymond F Morgan; David B Drake Journal: Ann Plast Surg Date: 2009-05 Impact factor: 1.539