A simple, quantitative method for spectroscopic detection of metformin using gold nanoclusters.

We synthesized bovine serum albumin (BSA)-stabilized gold nanoclusters (BSA-GNCs) and confirmed their ultra-small size using HRTEM (High-resolution Transmission Electron Microscope) and DLS (Dynamic Light Scattering). The fluorescence intensity of BSA-GNCs is "turned off" in the presence of Cu(II) metal ions. The resulting Cu(II)-mediated BSA-GNCs were utilized to detect metformin, a drug used to control diabetes. Metformin binds to and displaces Cu(II) ions from the BSA on the surface of the nanoclusters, which turns on the fluorescence of the nanoclusters. The interactions between the protein-stabilized nanoclusters were investigated in the absence and presence of Cu(II) using circular dichroism (CD) and Fourier-transform infrared spectroscopy (FTIR). Cu(II)-quenched BSA-GNCs had an extremely high sensitivity to detect metformin, with a low limit of detection (LOD) of 0.068 μM and a dynamic range of limit of quantification (LOQ = 10/3 LOD) of 0.22 to 11 μM. The ability of this novel "turn-on" nanosensor to detect metformin in human serum and urine samples was confirmed: the percentage recovery in fluorescence for spiked analyte ranged from 96.00-98.50% and 92.60-96.62% in human serum and urine samples, respectively. Thus, BSA-GNCs provide a valid, sensitive, specific fluorometric methodology for the detection of metformin in biomedical applications.