Generation of a Retina Reporter hiPSC Line to Label Progenitor, Ganglion, and Photoreceptor Cell Types.

Purpose: Early in mammalian eye development, VSX2, BRN3b, and RCVRN expression marks neural retinal progenitors (NRPs), retinal ganglion cells (RGCs), and photoreceptors (PRs), respectively. The ability to create retinal organoids from human induced pluripotent stem cells (hiPSC) holds great potential for modeling both human retinal development and retinal disease. However, no methods allowing the simultaneous, real-time monitoring of multiple specific retinal cell types during development currently exist.
Methods: CRISPR/Cas9-mediated homology-directed repair (HDR) in hiPSCs facilitated the replacement of the VSX2 (Progenitor), BRN3b (Ganglion), and RCVRN (Photoreceptor) stop codons with sequences encoding a viral P2A peptide fused to Cerulean, green fluorescent protein, and mCherry reporter genes, respectively, to generate a triple transgenic reporter hiPSC line called PGP1. This was accomplished by co-electroporating HDR templates and sgRNA/Cas9 vectors into hiPSCs followed by antibiotic selection. Functional validation of the PGP1 hiPSC line included the ability to generate retinal organoids, with all major retinal cell types, displaying the expression of the three fluorescent reporters consistent with the onset of target gene expression. Disaggregated organoids were also analyzed by fluorescence-activated cell sorting and fluorescent populations were tested for the expression of the targeted gene.
Results: Retinal organoids formed from the PGP1 line expressed appropriate fluorescent proteins consistent with the differentiation of NRPs, RGCs, and PRs. Organoids produced from the PGP1 line expressed transcripts consistent with the development of all major retinal cell types. Conclusions and Translational Relevance: The PGP1 line offers a powerful new tool to study retinal development, retinal reprogramming, and therapeutic drug screening. Copyright 2020 The Authors.

Introduction

Stem cells provide multiple avenues to address irreversible vision loss associated with retinal cell death caused by trauma or by disease. Common diseases, including age-related macular degeneration (AMD) and glaucoma, lead to irreversible blindness, costing billions of dollars in social welfare and lost productivity., Recent advancements in three-dimensional culture have led to the development of retinal organoids consisting of all major retinal cell types from human induced pluripotent stem cells (hiPSCs). These hiPSC-derived organoids can model normal neural retinal (NR) development and progression of retinal disease. Additionally, organoids provide a platform to screen drugs for the treatment of retinal degeneration., Finally, hiPSCs can potentially make unlimited numbers of specific retinal neurons for transplantation therapies.

The rapidly expanding use of hiPSCs to model human retina justifies the need for tools to monitor the appearance of specific cell types without interrupting normal development. For this purpose, CRISPR/Cas9 gene editing facilitates the insertion of cell type–specific reporters into hiPSCs. Although several hiPSCs with single reporter knock-ins exist,– these lines only detect terminally differentiated retinal cell types, which limits the ability to study progression of retinal development in real time. Having multiple cell type reporters inserted into the same genome will permit specific cell sorting and allow for the optimization of protocols that enrich for the development of particular retinal cell types. For example, one might need a large number of photoreceptors (PRs) or retinal ganglion cells (RGCs) to screen for drugs to treat AMD or glaucoma, respectively.,

To address these limitations, we used CRISPR/Cas9 to insert reporter genes encoding fluorescent proteins into the VSX2, BRN3b, and RCVRN loci in hiPSCs to make it possible to monitor the differentiation of neural retinal progenitors (NRPs), RGCs, and PR cells, respectively. VSX2 encodes a transcription factor that marks NRPs. In the mature NR, only postmitotic bipolar cells express VSX2.– RGCs represent the first mature NR cell type to develop, and most RGCs express the BRN3b transcription factor. PRs (rods and cones) express RCVRN, a protein important in the recovery phase of visual excitation. The cell type–specific expression pattern of these genes made them appropriate targets for fluorescent protein expression. Given their importance in retinal development and/or function, we sought to retain the function of both alleles of all three (VSX2, BRN3b, and RCVRN) genes in engineered hiPSCs by using viral P2A peptides to create fusion genes between the target and the fluorescent reporter that would self-cleave upon translation. Therefore, we inserted a P2A:Cerulean reporter into the VSX2 locus, a P2A:eGFP reporter into the BRN3b locus, and a P2A:mCherry reporter into the RCVRN locus by replacing the stop codon of the endogenous gene with the P2A:reporter.

Methods

Specific Single-Guide RNA Vector (sgRNA) Design

CRISPR specific guide RNAs (Supplementary Table S1) were individually cloned into a PX458 vector that includes the Streptococcus pyogenes Cas9 (#48138; Addgene, Watertown, MA) as described. To determine Cas9-sgRNA cutting efficiency, we transfected each vector into human embryonic kidney 293 (HEK293) cells. After 48 hours, HEK293 DNA was extracted and amplified by Q5 High-Fidelity PCR (M0494S; NEB, Ipswich, MA) using primers encompassing the sgRNA recognition site. The polymerase chain reaction (PCR) products were digested with T7 Endonuclease I (M0302S; NEB) as described. Successful Cas9 cleavage by sgRNAs resulted in two distinct bands in the T7E1 assay.

Homology-Directed Repair (HDR) Template Generation

To generate the HDR templates, the left and right homology arms (HAs) of each locus were amplified from the wild-type (WT) hiPSC genomic DNA (see Supplementary Table S2). The amplified HAs of VSX2, BRN3b, and RCVRN genes were inserted into the previously assembled vectors FRT.TK.Puro.FRT.pL451, LoxP.TK.Blast.LoxP.pL452, and FRT.TK.Neo.FRT.pL451 via Gibson Assembly (E2611L; NEB), respectively (vector sequences available upon request). These insertions were confirmed by DNA sequencing. The Cerulean fluorescent reporter gene was derived from pCS-membrane-CeruleanFP, a gift from Sean Megason (Addgene plasmid 53749). The GAP43-eGFP reporter gene was derived from pCAG-mGFP, a gift from Connie Cepko (Addgene plasmid 14757). The mCherry reporter gene was derived from pEF6V5:eGFP-CAAX-2A-mCherry, a gift from Steven Leach (Addgene plasmid 26901).

Insertion of Fluorescent Reporter Genes into Selected Loci

To generate the RCVRN/mCherry hiPSCs, we transfected 2.5 µg of the HDR template and 2.5 µg of the sgRNA vector into WT hiPSCs (hiPSC6.2, from Life Technologies, Carlsbad, CA; A18945) using a 4D-Nucleofector-X-Unit and the P3-Primary-kit (V4XP-3012; Amaxa, Cologne, Germany) based on the manufacturer's protocol. After transfection, cells were cultured with Essential 8 media plus 10 µM ROCK inhibitor (SCM075; Sigma, St. Louis, MO) overnight, with subsequent daily media changes without ROCK inhibitor. Antibiotic selection began after 48 hours with 100 µg/mL and slowly increased to 250 µg/mL of G418 (30234CR; Corning, Corning, NY) over the course of 1 week. DNA from resistant clones was extracted (D3025; Zymo Research, Irvine, CA) and screened for reporter integration by PCR and validated by DNA sequencing. A single RCVRN/mCherry hiPSC line was transfected with 1.25 µg each of the BRN3b:eGFP HDR template, the sgRNA vector to the BRN3b locus, the VSX2:Cerulean HDR template, and the sgRNA vector to the VSX2 locus using nucleofection as described above. Double antibiotic selection was performed using 100 µg/mL each of blasticidin (ant-bl-05; InvivoGen, San Diego, CA) and puromycin (ant-pr-1; InvivoGen) and slowly increased up to 250 µg/mL of each antibiotic over the course of 1 week to select for blasticidin/puromycin resistant colonies. All resistant clones were screened for reporter integration by PCR (see Supplementary Table S2) and validated by DNA sequencing.

Off-Target Screening

The PGP1 line was screened by selecting five high-scoring off-target sites for each sgRNA provided by Benchling (San Francisco, CA). Each potential sgRNA off-target site (Supplementary Table S3) was screened by High-Fidelity PCR (Q5 NEB, M0491L) with primers listed in Supplementary Table S4, and PCR products were sequenced by Eurofins Genomics (Louisville, KY). Each result was independently repeated five times.

Three-Dimensional Retinal Organoid Generation from the PGP1 Triple Targeted Line

PGP1 retinal organoids were created using the Zhong et al. protocol with modifications. Briefly, hiPSCs were incubated in 0.5 mM EDTA/DPBS (15575020; ThermoFisher, Waltham, MA) for 5 minutes at 37°C, prior to dissociation and culturing in mTeSR1 (85850; Stemcell Technologies, Vancouver, Canada) with 10 µM ROCK inhibitor (SCM075; Sigma) to form aggregates. The aggregates were gradually transitioned into neural induction medium (NIM) from day (D) 1 to D3 of differentiation, then cultured in NIM from D3 to D6. On D7, the aggregates were seeded on Matrigel (hESC qualified, Corning 354277)–coated dishes in NIM at an approximate density of 20 aggregates per cm2 and switched to Dulbecco's modified Eagle's medium (DMEM)/F12 (11320082; ThermoFisher) (3:1) supplemented with 2% Gem21 NeuroPlex (without vitamin A, 400-161; Gembio, Sacramento, CA), 1× NEAA (Non-Essential Amino Acids), and 1% antibiotic-antimycotic (15240062; ThermoFisher) on D16, with medium changes every third day. On D28 of differentiation, cells were detached, transferred to Petri dishes, and cultured in suspension in DMEM/F12 (3:1) supplemented with 2% Gem21 NeuroPlex, 1× NEAA (11140050; ThermoFisher), and 1% antibiotic-antimycotic (15240096; ThermoFisher). Within 3 to 5 days, cells began forming three-dimensional retinal organoids. The organoids were then mechanically separated from the rest of the cells using sharpened tungsten needles under a dissecting microscope. From that point on, the medium was changed twice a week. To culture the retinal organoids long term, the medium was supplemented with 10% fetal bovine serum (FBS) (26140079; ThermoFisher), and 2 mM GlutaMax (35050061; ThermoFisher) beginning on D42.

Reverse Transcription–PCR (RT-qPCR) Analysis of Retinal Organoids

RNA isolation of retinal organoids was done using Quick RNA miniprep (R1057; Zymo Research). Reverse transcription was performed using the ImProm-II Reverse Transcription System (A3800; Promega, Madison, WI). RT-qPCR was performed with GoTaq qPCR Master Mix (A6001; Promega) using a CFX Connect Bio-Rad qPCR System. Forty cycles were run at 95°C denaturation for 40 seconds, 60°C annealing for 40 seconds, and 72°C extension for 60 seconds, using primers listed in Supplementary Table S5. The expression levels of genes were normalized to GAPDH messenger RNA (mRNA) levels and analyzed using the delta-delta Ct method with significant differences revealed by a two-tailed Student t test. Error bars in each figure represents the standard error of the mean (SE) of three individual experiments.

Immunofluorescence of the Retinal Organoids

Organoids were fixed with 4% paraformaldehyde (15710; ThermoFisher) for 20 minutes at room temperature, incubated in 30% sucrose overnight at 4°C, and embedded in OCT (95057-838; VWR, Radnor, PA). Organoids were cryosectioned at 15 µm and immunofluorescence was performed using antibodies listed in Supplementary Table S6 with images acquired using a Nikon (Melville, NY) Eclipse 80i microscope or Zeiss (Jena, Germany) LSM 710 Laser Scanning Confocal System. Residual fluorescence from the fluorescent proteins failed to survive our fixation and embedding protocol and therefore did not interfere with immunofluorescence studies (Supplementary Figs. S1, S2).

Fluorescence-Activated Cell Sorting (FACS) Analysis of the Retinal Organoids

Thirty organoids each from D55 and D135 were enzymatically dissociated in Accutase (AT104; Innovative Cell Technologies, San Diego, CA), and filtered through a 40-µm strainer (22-363-547; ThermoFisher) to get single cells and resuspended in ice-cold 5% FBS/1× HBSS (Hanks Balanced Salt Solution) (14-025-092; ThermoFisher) at a concentration of 10 million cells/mL. These cells were filtered again through a second strainer (08-771-23; ThermoFisher) prior to sorting. At D55, single cells were sorted for Cerulean positive (Cerulean+) and negative (Cerulean−) populations, as well as the eGFP-positive (eGFP+) and eGFP-negative (eGFP−) populations using a FACSMelody Cell Sorter (BD Biosciences, Franklin Lakes, NJ). For the D135, single cells were sorted for mCherry-positive (mCherry+) and mCherry-negative (mCherry−) populations. The sorted populations were used to measure mRNA transcripts for Cerulean, VSX2, eGFP, BRN3b, mCherry, RCVRN, and GAPDH via RT-qPCR (Supplementary Table S7). Gates for sorting disaggregated retinal organoids were established in a previous experiment using HEK293 cells transiently transfected with an expression plasmid for Cerulean, eGFP, or mCherry. FACS was conducted 48 hours after transfection to determine the appropriate gates for each fluorescent protein (Supplementary Fig. S3). In addition, organoids produced from WT hiPSCs were used to established negative gates.

Results

Creation of the Neural Retinal Progenitor/Retinal Ganglion/Photoreceptor (PGP1) Reporter

CRISPR/Cas9-mediated HDR (Fig. 1A) facilitated the replacement of the endogenous stop codons of the VSX2, BRN3b, and RCVRN genes with a P2A peptide fused to a fluorescent reporter gene. Briefly, hiPSCs were nucleofected with a S pyogenes Cas9 expression vector (containing appropriate sgRNAs) and HDR templates targeting each locus. Antibiotic resistant clones were tested by DNA sequence analysis of two PCR amplicons (Supplementary Table S8) encompassing the 5′ and 3′ ends of the targeted modification with at least one PCR primer outside of the HA (Figs. 1B–D). The RCVRN locus was first targeted independently, resulting in 48 G418 resistant hiPSC clones, with three (6.25%) clones verified as correctly targeted by DNA sequencing of PCR amplicons (Fig. 1D). Simultaneous targeting of the VSX2 and BRN3b loci from one of these clones yielded 144 clones resistant for both puromycin and blasticidin. PCR genotyping followed by DNA sequencing identified four (2.8%) of these triple antibiotic-resistant clones (56, 101, 108, and 122) as correctly targeted at all three targeted loci. We randomly designated one (122) of these triple targeted hiPSC clones (PGP1) for further molecular and functional analyses.

Figure 1.

Creation of the neural retinal progenitor/retinal ganglion cell/photoreceptor (PGP1) reporter hiPSC line by CRISPR/Cas9 genome editing. (A) Schematic illustration of the generalized CRISPR/Cas9-mediated insertion strategy. CRISPR/Cas9 mediated the replacement of the endogenous STOP codon of VSX2 (B), BRN3b (C), and RCVRN (D) loci with P2A:Cerulean, P2A:eGFP, and P2A:mCherry by homologous recombination in WT hiPSCs. Following nucleofection and triple antibiotic selection for puromycin (PURO), blasticidin (BLAST), and G418 (NEO), the resistant clones were screened by PCR with primer sets. (B) FW1/RV1 (forward primer [FW] located outside VSX2 5′HA and reverse primer [RV] located inside Cerulean) with the expected band size of 2.1 kb and FW2/RV2 (inside PURO to outside VSX2 3′HA) with the expected band size of 1.9 kb. (C) FW3/RV3 (outside BRN3b 5′HA to inside membrane tagged eGFP) with the expected band size of 1.7 kb and FW4/RV4 (inside BLAST to outside BRN3b 3′HA) with the expected band size of 1.5 kb. (D) FW5/RV5 (outside RCVRN 5′HA to inside mCherry) with the expected band size of 1.2 kb and FW6/RV6 (inside NEO to outside RCVRN 3′HA) with the expected band size of 1.5 kb. The WT hiPSC was used as control where no bands were seen. All the positive PCR bands were verified by sequencing. The original gels that were cropped for clarity in this figure (with white spaces between nonadjacent lanes) can be seen in their entirety in Supplementary Figure S9. Primer information listed in Supplementary Table S8.

A three-primer PCR strategy, using the forward primer outside 5′HA, with two different reverse primers made it possible to determine if the PGP1 line was heterozygous or homozygous at each locus. All three loci yielded PCR amplicons with expected band sizes for WT and targeted alleles (Fig. 2). Sequence analysis from the WT PGP1 alleles failed to reveal indel mutations at the sgRNA cut sites (Supplementary Fig. S4). Additionally, DNA sequencing of PCR fragments, amplified by primers surrounding predicted high-scoring off-target sites for each sgRNA, revealed no off-target mutations in PGP1 (Supplementary Table S3).

Figure 2.

Determining the zygosity of the PGP1 line. In each case, a three-primer PCR strategy determined whether the PGP1 line was homozygous or heterozygous at the targeted loci. For the VSX2 locus, primer FW1 is located outside the VSX2 5′HA, RV1 is located inside Cerulean, and RV2 is located outside the VSX2 3′HA. (A) The unedited VSX2 allele (FW1/RV2) should generate a 2.6-kb band, (A′), whereas the edited VSX2 allele (FW1/RV1) should generate a 2.1-kb band. (A′′) A gel image showed bands of 2.6 kb and 2.1 kb for the PGP1 line, whereas the WT hiPSC showed a 2.6-kb band indicating that PGP1 is heterozygous at the VSX2 locus. For the BRN3b locus, FW3 is located outside the BRN3b 5′HA, RV3 is located inside the eGFP, and RV4 is located outside the BRN3b 3′HA. (B) The unedited BRN3b allele (FW3/RV4) should generate a 2.4-kb band, (B′) whereas the edited BRN3b allele (FW3/RV3) should generate a band of 1.7 kb. (B′′) A gel image showed bands of 2.4 kb and 1.7 kb for the PGP1 line, whereas the WT hiPSC showed only a 2.4-kb band, showing that PGP1 is heterozygous at the BRN3b locus. For the RCVRN locus, FW5 is located outside the RCVRN 5′HA, RV5 is located inside mCherry, and RV6 is located outside the RCVRN 3′HA. (C) The unedited RCVRN allele (FW5/RV6) should generate a 2.2-kb band, (C′) whereas the edited RCVRN allele (FW5/RV5) should generate a 1.2-kb band. (C′′) A gel image showed bands of 2.2 kb and 1.2 kb for the PGP1 line, whereas the WT hiPSC showed only a 2.2-kb band, indicating that PGP1 is heterozygous at the RCVRN locus. The original gels that were cropped for clarity in this figure (with white spaces between nonadjacent lanes) can be seen in their entirety in Supplementary Figure S10. Primer information listed in Supplementary Table S8.

Functional Analysis of the Fluorescent Reporters in the PGP1

To test the function of PGP1 retinal reporter genes, we created retinal organoids from PGP1.hiPSCs, using a previously described protocol. Only the VSX2:Cerulean reporter expressed at D20 of differentiation with fluorescence restricted to developing eye field domains (Supplementary Fig. S5). Manually isolated eye field domains (isolated between D20 and D24) formed spherical cups (Supplementary Fig. S6) with both Cerulean and eGFP expression by D55 (Figs. 3A, 3B). In the BRN3b reporter, the eGFP contained a GAP43 palmitoylation tag derived from the plasmid pCAG-mGFP to drive eGFP to the cell membrane, which highlighted RGC axon bundles (Figs. 3B, 3D). At D55, sparse mCherry fluorescence, driven by RCVRN promoter, appeared sporadically throughout the organoids (Figs. 3C, 3D). A merged image of all three signals shows the restriction of fluorescent expression to distinct cells with little evidence of overlapping reporter expression (Fig. 3D, Supplementary Movie S1). Scanning parameters for Figure 3 and the Supplementary Movie S1 are supplied in Supplementary Table S9.

Figure 3.

Functional analysis of the fluorescent reporters in the PGP1 line via retinal organoid formation. Single three-dimensional retinal organoids derived from the PGP1 hiPSC line were visualized by fluorescent microscopy at D55 (A, B, C, D), D95 (E, F, G, H), and D135 (I, J, K, L) of differentiation. Organoids were visualized with a 458-nm laser to excite Cerulean, driven by the VSX2 promoter (A, E, I); a 488-nm laser to excite eGFP, driven by the BRN3b promoter (B, F, J); and both 543-nm and 633-nm lasers to excite mCherry, driven by the RCVRN promoter (C, G, K). Composite images represent the merger of all three fluorescent signals (D, H, L). The eGFP signal was targeted to the cell membrane by a GAP43 tag to allow for visualization of ganglion cell axon bundles (white arrows in B, D). As organoids matured from D55 to D135, the number of cells expressing mCherry dramatically increased (compare C and K), and the eGFP+ cells populated the interior of the organoid (J) while the mCherry+ cells occupied the organoid periphery (K). Magnification bar: 100 µm. Scanning parameters are in Supplementary Table S9.

As the organoids matured, the ratio of cells expressing different fluorescent proteins changed. At D55, the majority of cells in the organoid expressed Cerulean, but as the organoids matured, the proportion of Cerulean expressing cells decreased (Figs. 3A, 3E, 3I). By D95, the eGFP-expressing axon bundles had disappeared, but punctate eGFP staining remained within the organoid and overall eGFP expression declined by D135 (Figs. 3F, 3J). This real-time decline in eGFP expression at later stages of organoid development correlates with the decline in the BRN3b mRNA levels (see Fig. 5E). Other laboratories have noted the challenge of long-term survival of retinal ganglion cells in three-dimensional retinal organoid culture., The loss of eGFP-expressing retinal ganglion cell axon bundles in organoid culture may relate to a lack of synaptic contact to target brain regions., Although only weak mCherry fluorescence appeared at D55, mCherry expression increased such that by D135, large areas of the organoid displayed mCherry fluorescence (Figs. 3C, 3G, 3K). Cerulean, eGFP, and mCherry signals did not appear to overlap either in the whole mounts or in the optical section Z-stack (Supplementary Movie S1).

Figure 5.

Retinal development is recapitulated in the differentiating PGP1 hiPSC-derived retinal organoids. (A–C) Undifferentiated PGP1 hiPSCs day 0 (D0, blue) and differentiated PGP1 retinal organoids at day 55 (D55, black) were used to measure mRNA levels of various markers via RT-qPCR normalized to GAPDH. (A) Expression of stem cell markers , ; (B) NRP markers ,,; (C) Retinal pigment epithelium (RPE) markers MITF, BEST1. (D–F) PGP1 hiPSCs at D0 and hiPSCs retinal organoids at D55, D95, and D166 of differentiation were used to measure mRNA levels via RT-qPCR normalized to GAPDH. (D) NRP and bipolar marker VSX2, (E) RGC marker , and (F) PR marker . As organoid differentiation progressed from D0 to D55, stem cell markers significantly decreased and markers of retina and RPE differentiation significantly increased. Compared to D55, the PR marker RCVRN progressively increased in its mRNA expression, whereas the NRP marker VSX2 and the RGC marker BRN3b gradually decreased in the older retinal organoids. Error bars represent standard error of the mean (SE), paired t test. *P < 0.05. **P < 0.005. ***P < 0.0005. Primer information is listed in Supplementary Table S5.

To confirm that the observed fluorescent proteins identified the targeted cell types, we sorted organoid cells based on fluorescent protein expression. At D55, the organoids contained large numbers of Cerulean and eGFP+ cells but relatively fewer mCherry+ cells. By D135, the organoids contained abundant mCherry+ cells. Considering this, we performed FACS on single cell suspensions to collect Cerulean+ and eGFP+ (Figs. 4A, 4D) cells from D55 and mCherry+ (Fig. 4G) cells from D135 organoids. At D55, Cerulean+ and eGFP+ cells comprised approximately 11.3% and 4.9% of the single cells from disaggregated organoids, respectively. At D135, approximately 53% of organoid cells were mCherry+. We also sorted organoids from WT hiPSCs using the same gates used for the PGP1-derived organoids and found no cells within the Cerulean (Fig. 4J), eGFP (Fig. 4K), or mCherry (Fig. 4L) gates. Because FACS can only analyze single cells, it remains possible that certain organoid cell types exhibit increased resistance to single cell dissociation that could bias our quantitative analysis. However, this bias should not influence comparative analysis between expressing and nonexpressing cells for any single fluorescent protein.

Figure 4.

Confirmation of PGP1-derived retinal organoids corresponded to the appropriately targeted cell types. At D55 (A–F, J–L) and D135 (G–I), the retinal organoids were dissociated into single cells and used for FACS analysis. At D55, (A) the Cerulean+ population was sorted from the Cerulean− population. RT-qPCR analysis revealed that the Cerulean+ cells expressed significantly more Cerulean mRNA (B) and VSX2 mRNA (C) than the Cerulean− cells. Also, at D55, (D) the eGFP+ population was sorted from the eGFP− population. RT-qPCR analysis demonstrated that the eGFP+ cells expressed significantly more eGFP mRNA (E) and BRN3b mRNA (F) than the eGFP− cells. At D135, (G) the mCherry+ population was sorted from the mCherry− population. RT-qPCR analysis revealed that the mCherry+ cells expressed significantly more mCherry mRNA (H) and RCVRN mRNA (I) than mCherry− cells. As a negative control, retinal organoids (D55) derived from WT hiPSC cells were run through the FACS and analyzed with the gates used for Cerulean (J), eGFP (K), and mCherry (L) with no cells occupying those gates. All RT-qPCR data were normalized to GAPDH expression. Error bars represent standard error of the mean (SE). Primer information listed in Supplementary Table S7.

We performed RT-qPCR and immunofluorescence to determine if fluorescent reporter expression correlated with the expression of the targeted gene in sorted samples. The transcript analysis revealed that the sorted Cerulean+ population (Fig. 4A) expressed significantly more Cerulean mRNA than the Cerulean− population (Fig. 4B). Likewise, the Cerulean+ population expressed approximately three times more VSX2 mRNA than the Cerulean− population (Fig. 4C). The eGFP+ sorted population (Fig. 4D) expressed 22-fold more eGFP mRNA and 9.1-fold more BRN3b mRNA than the eGFP− population (Figs. 4E, 4F). On D135, the mCherry+ (Fig. 4G) population expressed approximately 4.9 times more mCherry mRNA and 1.9 times more RCVRN mRNA than the mCherry− population (Figs. 4H, 4I). For VSX2 and BRN3b, the majority of expressing cells appeared in the Cerulean+ and eGFP+ populations, respectively. The appearance of relatively more RCVRN transcripts in the mCherry− population than BRN3b transcripts in the eGFP− or VSX2 transcripts in the Cerulean− populations suggests that not all RCVRN-expressing cells express mCherry. Double immunohistochemical analysis of D55 organoids revealed colabeling of BRN3 and GFP (Supplementary Figs. S7A–C), whereas D163 organoids showed colabeling of VSX2 and GFP (Supplementary Figs. S7D–F). Note that in both of the above cases, our antibody cannot distinguish GFP from Cerulean. Although antibodies for RCVRN colabel cells that express mCherry in D163 organoids (Supplementary Figs. S7G–I), the organoids exhibited some RCVRN-expressing cells that failed to express mCherry. However, this same analysis showed that all mCherry+ cells coexpressed RCVRN. The RCVRN+/mCherry− proportion of cells exhibited organoid-to-organoid variability (Supplementary Fig. S8). All of the positive and negative sorted populations for each fluorescent protein were tested by RT-qPCR in triplicate (Figs. 4B, 4C, 4E, 4F, 4H, 4I). These data demonstrate that the reporters faithfully reveal the target gene-expressing cells. Although we did not conduct FACS analyses on organoids formed by any of the other triple targeted hiPSC lines, we did confirm that clones 56 and 101, in addition to PGP1 (122), formed organoids expressing all three fluorescent proteins.

PGP1-Derived Retinal Organoids Contain All Major Retinal Cell Types

A comparison of transcripts expressed by PGP1 hiPSCs at D0 of differentiation with those expressed by PGP1-derived retinal organoids at D55 revealed a decrease in pluripotency-related genes and an increase in retinal differentiation-related genes. Pluripotency transcripts for OCT4 (POU5F1) and NANOG expressed abundantly at D0 (Fig. 5A). In contrast, the NRP transcripts (PAX6, SIX3, and LHX2) (Fig. 5B) and retinal pigment epithelium (MITF and BEST1) transcripts (Fig. 5C) increased significantly during early retinal organoid differentiation at D55. In addition, a sequential expression of NRPs (VSX2) and RGCs (BRN3b) revealed a decline, whereas the level of PRs’ transcript (RCVRN) increased significantly from D55 to D166 of differentiation (Figs. 5D, 5E, 5F).

PGP1-derived organoids contained cells of all major retinal cell types. At D55, organoids expressed the eye field precursor protein RX (Fig. 6A); NRP markers PAX6, SIX3, and VSX2 (Figs. 6B–D); and the proliferation marker MCM2 (Fig. 6E) throughout the D55 organoid tissue. In contrast, the expression of BRN3b (marking RGCs) localized interiorly (outlined in Fig. 6F), as did AP2-α (amacrine cell marker) by D70 (outlined in Fig. 6G). Although RCVRN (PR marker) initially appeared throughout the organoid, by D70, RCVRN appeared near the organoid periphery (Fig. 6H). At D95, PROX1 (horizontal cell marker) was located below the putative outer nuclear layer (Fig. 6I). CRALBP, a marker of the late-differentiating Müller glia cells, appeared through the full thickness of the retinal organoids at D163 (Fig. 6J). Although VSX2 expression initially characterized proliferating (MCM2 positive) NRP cells, VSX2 appeared later in nonproliferating (MCM2 negative) bipolar cells. These VSX2 positive/MCM2 negative bipolar cells increased in abundance by D163 (Figs. 6K, 6L).

Figure 6.

Retinal organoids differentiated from PGP1 line contain all major retinal cell types. At D55 of retinal organoid differentiation, the organoids were analyzed by immunohistochemistry using antibodies for the eye-field precursor marker RX (A), neuronal markers PAX6 (B) and SIX3 (C), the NRP marker VSX2 (D), the proliferation marker MCM2 (E), and the RGC marker BRN3 (F). At D70 of the retinal organoid differentiation, the organoids contained the amacrine cell marker AP2-α (G) and the photoreceptor marker RCVRN (H). The organoids contained the horizontal cell marker PROX-1 (I) at D95 and the Müller glia cell marker CRALBP (J) at D163. Also, at D163, the organoids contained the bipolar cell marker VSX2+/MCM2– (K, L). (L) An enlarged view of the boxed area in (K) where VSX2+/MCM2+ (white arrowheads) cells represent NRPs and the VSX2+/MCM2– (white arrows) cells represent bipolar cells at D163. Dotted lines emphasize that the major expression domains of Brn3b (F), AP2α (G), and Prox1 (I) appear on the interior of the organoids, whereas RCVRN (H) is predominantly on the organoid periphery. Magnification bar: 100 µm (A–K); 25 µm (L). Antibodies used for immunohistochemistry listed in Supplementary Table S6.

Discussion

We created an hiPSC line (PGP1) for real-time analysis of NR differentiation with multiple retinal cell type–specific reporters via CRISPR/Cas9 genome editing. Furthermore, we functionally characterized PGP1 both by fluorescent protein expression and by differentiation into all major retinal cell types during retinal organoid formation. Although previous reports described single retinal cell type–specific reporters, to our knowledge, PGP1 represents the first triple targeted retinal reporter hiPSC line.– The ability of the PGP1 to facilitate visual observation of retinal differentiation, without compromising retinal organoid formation typical of WT hiPSCs, makes this clone particularly useful for several applications.

PGP1 provides a powerful tool for real-time retinal disease modeling. For example, hiPSC-derived organoids from patients with mutations leading to retinitis pigmentosa and Leber congenital amaurosis have provided important insights about the pathobiology of these diseases.– Likewise, RGCs with specific mutations, differentiated from patient-derived hiPSCs, have provided models for optic atrophy and glaucoma., The relative ease of creating specific mutations with CRISPR/Cas9 genome editing should make it possible to take advantage of the cell type–specific reporters in PGP1 when modeling genetic retinal diseases.

PGP1-derived retinal organoids will provide a platform to screen drugs to treat retinal damage. A number of different physical or chemical insults can result in PR injury, making the search for compounds that can prevent or minimize such damage very important. Retinal organoids made from mouse induced pluripotent stem cells, with a Nrl-eGFP reporter to label rod PRs, facilitated the testing of compounds to protect PRs from 4-hydroxytamoxifen-induced degeneration. Additionally, the recently reported three-dimensional automated reporter quantification technology would make screening drugs that affect retinal development and/or survival straightforward in PGP1-derived organoids. Therefore, the use of PGP1 hiPSCs for such a purpose would have the added advantage of simultaneous real-time monitoring of NRPs and PRs without having to fix the organoid and terminate the experiment. In fact, the loss of fluorescence in the PGP1-derived organoids postfixation (Supplementary Fig. S1) presents a small limitation of using this line for terminal analysis. However, immunohistochemistry can reveal mCherry and GFP/Cerulean expression in fixed organoid tissue (Supplementary Fig. S7). In fact, we consider the loss of reporter protein fluorescence postfixation as an advantage because it removes a potentially interfering signal from subsequent immunohistochemical analysis with fluorescent secondary antibodies.

PGP1 hiPSCs will facilitate protocol optimization to achieve the differentiation and/or survival of specific retinal cell types. For example, improved mature PR yields and laminar stratification resulted from the use of stirred-tank bioreactors, due to increased cell proliferation and decreased apoptosis. This study used immunohistochemical and FACS-mediated immunopurification of organoid-derived cell types. PGP1-derived organoids would permit similar protocol optimization for multiple cell types in real time without relying on antibody labels. Additionally, the decline of ganglion axon bundles in late-stage organoids observed in our line and other published observations– presents a challenge for culturing RGCs with our current organoid protocol. The PGP1-dervied organoids would permit protocol optimization for long-term survival of RGCs and axon bundles in late-stage organoids. Therefore, the ability to continuously monitor RGCs and PRs should improve optimization for long-term survival of these cell types.

PGP1 hiPSCs could provide an effective strategy for improved purification of specific cell types. In direct differentiation protocols to achieve particular retinal cell types from hiPSCs, purifying fully differentiated, mature cell types remains an issue. The purification of specific retinal neurons can often facilitate downstream analysis or transplantation studies. Recombinant AAV (Adeno-associated Virus) viruses containing a GFP reporter driven by an L/M cone-specific promoter led to the successful FACS isolation of cone PRs from both human fetal retina and from hiPSC-derived retinal organoids. A similar strategy used recombinant lentivirus to achieve GFP expression from the PR-specific (IRBP) promoter to achieve FACS purification of hiPSC-derived PR cells prior to transplantation into immunocompromised mice. Although both of these reports relied on virus-mediated reporter gene expression, others have adopted the use of endogenous reporters. For example, hiPSCs engineered with a P2A peptide-mCherry reporter into the BRN3b locus facilitated the purification of viable RGCs from retinal organoids by FACS. These examples all attest to the potential of the PGP1 line for purification of specific retinal cell types. However, the unique feature of the PGP1 line lies in its potential for simultaneously purifying NRPs, RGCs, PRs, and bipolar cells.

hiPSC lines with multiple cell type–specific reporters could serve as a powerful tool for proof-of-concept studies and for tracking retinal-specific cell fates posttransplantation in animal models. For example, transplanting human NRPs into a mouse glaucoma model increased host RGC survival. In the future, PGP1-derived cells will make it possible to evaluate the fate of transplanted cells in animal models in vivo. In these transplants, PGP1-derived NRPs, and later bipolar cells, would exhibit blue fluorescence, whereas RGCs or PRs derived from the transplant would exhibit green or red fluorescence, respectively. In another study, transplantation of GFP-labeled human PRs into a rat model of retinitis pigmentosa demonstrated restoration of the host rod function. Although these authors reported integration of GFP-positive PRs into the outer nuclear layer, the recent realization of cytoplasmic transfer from donor to host cells in PR transplantation, necessitates further confirmation of donor cell integration. Transplantation of PGP1-derived NRPs into an animal model of retinal degeneration would only result in GFP+ ganglion cells or mCherry+ PRs if these NRPs differentiated into these cell types in situ. These experiments could provide more definitive proof of donor cell integration and could also serve as an indicator of both cell survival and cell fate of the transplanted NRPs.

In summary, we demonstrate a CRISPR/Cas9 strategy to target the expression of multiple fluorescent reporter genes into endogenous loci in hiPSCs. In doing so, we created a triple transgenic hiPSC line (PGP1) and tested the function of this line by directed differentiation into three-dimensional retinal organoids. Organoids produced from the PGP1 line expressed Cerulean in NRPs and bipolar cells, membrane-targeted eGFP in RGCs, and mCherry in PRs. In addition, PGP1-derived retinal organoids contained all major retinal cell types. The usefulness of this strategy extends to virtually any cell type–specific gene, limited only by the number of different fluorescent reporters available for simultaneous analysis. The PGP1 line and subsequent hiPSC lines developed using this approach hold great promise for studying retinal development, disease modeling, drug screening, and preclinical transplantation studies.