Miles C Smalley1, Joe Olivi2, Krisi A Causa3, Manoj Pathak4, Cindy L Austin5, Simon J Thompson5. 1. Mercy Hospital-Springfield, Burn ICU 1235 E Cherokee, Springfield, MO 65804, USA. 2. Northwest Physicians, Surgical Services 601 W Maple Ave, Springdale, AR 72764, USA. 3. Mercy Hospital-Springfield, General & Trauma Surgery 1965 S Fremont, Springfield, MO 65804, USA. 4. Murray State University, Department of Mathematics and Statistics 102 Curris Center, Murray, KY 42071, USA. 5. Mercy Hospital-Springfield, Trauma & Burn Research 1235 E Cherokee, Springfield, MO 65804, USA.
Abstract
BACKGROUND: Burn injuries can induce distinct, systemic inflammatory and immunological responses which occur acutely up to 72 hrs or chronically after 24 hrs. Previously published literature showed a dramatic increase in whole blood histamine values within 24 hrs of a thermal injury. However, the data is limited due to infrequent monitoring, resulting in statistically insignificant findings. The goal of this study was to determine localized histamine fluctuations for 6 consecutive days in a successive group of patients admitted immediately after a burn. METHOD: Using blood plasma from 7 patients (average total burn surface area 24.7%), we examined histamine within an average 4.1 (± 0.3) hrs from burn injury, by means of a monoclonal-based competitive binding enzyme immunoassay. Histamine values were normalized to patient baselines prior to determining overall averages. Patient vitals and electrolyte values were extracted from the electronic health record. A two-tailed student t-test was used to compare values with p-value ≤ 0.05 considered statistically significant using statistical software R. RESULTS: The histamine values were significantly higher than patient baseline values up to 48 hrs (p-value ≤ 0.05), followed by a return to baseline values from approximately 3 days post-injury. Heart rates were within normal values up until 72 hrs. Hematocrit and hemoglobin began within normal values, dropped at 72 hrs, and reduced significantly from 96 hrs post-injury. The electrolyte calcium began within the normal range, and then was significantly less than the baseline value from 96 hrs post-injury. CONCLUSIONS: We have shown a distinct and significant increase in histamine plasma levels within 48 hrs after a moderate burn injury. IJBT
BACKGROUND: Burn injuries can induce distinct, systemic inflammatory and immunological responses which occur acutely up to 72 hrs or chronically after 24 hrs. Previously published literature showed a dramatic increase in whole blood histamine values within 24 hrs of a thermal injury. However, the data is limited due to infrequent monitoring, resulting in statistically insignificant findings. The goal of this study was to determine localized histamine fluctuations for 6 consecutive days in a successive group of patients admitted immediately after a burn. METHOD: Using blood plasma from 7 patients (average total burn surface area 24.7%), we examined histamine within an average 4.1 (± 0.3) hrs from burn injury, by means of a monoclonal-based competitive binding enzyme immunoassay. Histamine values were normalized to patient baselines prior to determining overall averages. Patient vitals and electrolyte values were extracted from the electronic health record. A two-tailed student t-test was used to compare values with p-value ≤ 0.05 considered statistically significant using statistical software R. RESULTS: The histamine values were significantly higher than patient baseline values up to 48 hrs (p-value ≤ 0.05), followed by a return to baseline values from approximately 3 days post-injury. Heart rates were within normal values up until 72 hrs. Hematocrit and hemoglobin began within normal values, dropped at 72 hrs, and reduced significantly from 96 hrs post-injury. The electrolyte calcium began within the normal range, and then was significantly less than the baseline value from 96 hrs post-injury. CONCLUSIONS: We have shown a distinct and significant increase in histamine plasma levels within 48 hrs after a moderate burn injury. IJBT
Authors: M L Hauhouot-Attoungbre; W C H Mlan; N A Edjeme; H Ahibo; B Vilasco; D Monnet Journal: Ann Biol Clin (Paris) Date: 2005 Jul-Aug Impact factor: 0.459
Authors: Marc G Jeschke; Gerd G Gauglitz; Gabriela A Kulp; Celeste C Finnerty; Felicia N Williams; Robert Kraft; Oscar E Suman; Ronald P Mlcak; David N Herndon Journal: PLoS One Date: 2011-07-18 Impact factor: 3.240