| Literature DB >> 32712920 |
Timothy Wiryaman1, Navtej Toor2.
Abstract
Selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) is a widely used technique for studying the structure and function of RNA molecules. It characterizes the flexibility of single nucleotides in the context of the local RNA structure. Here we describe the application of SHAPE-MaP (mutational profiling) to study different conformational states of the group II intron during the self-splicing reaction.Entities:
Keywords: Chemical structure probing; Group II intron; Next-generation sequencing; RNA structure; SHAPE-MaP
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Year: 2021 PMID： 32712920 PMCID： PMC7510887 DOI： 10.1007/978-1-0716-0716-9_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745