Sequential aqueous two-phase system for simultaneous purification of cyanobacterial phycobiliproteins.

A logical framework was used for designing a top-down strategy for cyanobacterial phycobiliprotein purification. The purification scheme is based on the non-chromatographic technique, known as aqueous two-phase system. The scheme was optimized at every stage to enhance the recovery yield with the highest purity. We tested this strategy on four cyanobacteria, two containing only phycocyanin and allophycocyanin (Arthrospira platensis AICB49, Synechocystis sp. AICB51) and two that have an extra phycobiliprotein, namely phycoerythrin (Fremyella sp. UTEX481, Coelomoron pussilum AICB1012). The results showed that the recovery efficiency of the phycobiliproteins is strongly influenced by the phycobilisome composition. For the first two strains the recovery yield of both phycocyanin and allophycocyanin was >80%, with an analytical purity grade for phycocyanin (>4.2) and a reactive purity grade for allophycocyanin (>2.9). The recovery yield of phycoerythrin was lower but compensating with an increase in purity, 5.2 for Fremyella and 4.5 for C. pussilum.