Hongnan Liu1,2,3, Miaomiao Bai1,4, Kang Xu1, Jian Zhou1, Xiaofeng Zhang2, Rong Yu2, Ruilin Huang1, Yulong Yin1,2,4. 1. National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Key Laboratory of Agro-ecological Processes in Subtropical Region, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center for Healthy Breeding of Livestock and Poultry, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China. 2. Hangzhou King Techina Technology Company Academician Expert Workstation, Hangzhou King Techina Technology Co. Ltd, Hangzhou, China. 3. Anhui Tianyuanju E-commerce Co. Ltd, Fuyang, China. 4. College of Animal Science, South China Agricultural University, Guangzhou, China.
Abstract
BACKGROUND: Coated nano zinc oxide (Cnz) is a new feed or food additive, which is a potential replacement for a pharmacological dose level of ZnO. This study evaluated the positive effects of different concentrations of Cnz on the intestinal bacterial core, enterobacterial composition and mucosal barrier function in a pig model. RESULTS: Microbiota sequencing results showed that Cnz could significantly alter the intestinal microbiota composition and metabolism. Besides increasing the richness indices (ACE and Chao1), 10% Cnz could protect the intestinal mucosal barrier through increasing the expression of occludin and zonula occludens-1 in the small intestine, increase the abundance of Lachnospiraceae UCG-004 and decrease the abundance of Ruminococcus flavefaciens compared to high ZnO diet and 5% Cnz material. CONCLUSIONS: Cnz material at 10% supplementation is more effective than a level of 5% Cnz in increasing intestinal barrier through affecting gut microbiota.
BACKGROUND: Coated nano zinc oxide (Cnz) is a new feed or food additive, which is a potential replacement for a pharmacological dose level of ZnO. This study evaluated the positive effects of different concentrations of Cnz on the intestinal bacterial core, enterobacterial composition and mucosal barrier function in a pig model. RESULTS: Microbiota sequencing results showed that Cnz could significantly alter the intestinal microbiota composition and metabolism. Besides increasing the richness indices (ACE and Chao1), 10% Cnz could protect the intestinal mucosal barrier through increasing the expression of occludin and zonula occludens-1 in the small intestine, increase the abundance of Lachnospiraceae UCG-004 and decrease the abundance of Ruminococcus flavefaciens compared to high ZnO diet and 5% Cnz material. CONCLUSIONS:Cnz material at 10% supplementation is more effective than a level of 5% Cnz in increasing intestinal barrier through affecting gut microbiota.
Authors: Anatoly V Skalny; Michael Aschner; Xin Gen Lei; Viktor A Gritsenko; Abel Santamaria; Svetlana I Alekseenko; Nagaraja Tejo Prakash; Jung-Su Chang; Elena A Sizova; Jane C J Chao; Jan Aaseth; Alexey A Tinkov Journal: Int J Mol Sci Date: 2021-12-03 Impact factor: 5.923