A Delkhosh1, H Shoorei2, V Niazi3, M Delashoub4,5, M Neshat Gharamaleki6, M Ahani-Nahayati3, Y Koohestani Dehaghi7, Sha Raza8, Mm Hassanzadeh Taheri2, M Mohaqiq9, F Abbasgholizadeh10. 1. Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, West Azerbaijan Province, Urmia, Iran. 2. Department of Anatomical Sciences, Faculty of Medicine, 125609Birjand University of Medical Sciences, South Khorasan Province, Birjand, Iran. 3. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, 556492Shahid Beheshti University of Medical Sciences, Tehran, Iran. 4. Department of Veterinary Basic Sciences, 201583Islamic Azad University, Tabriz Branch, Tabriz, Iran. 5. Biotechnology Research Center, 201583Islamic Azad University, Tabriz Branch, Tabriz, Iran. 6. Department of Clinical Sciences, Faculty of Veterinary Medicine, 201583Islamic Azad University, Tabriz Branch, Tabriz, Iran. 7. Department of Anatomical Sciences, Faculty of Medicine, 37554Guilan University of Medical Sciences, Gilan Province, Rasht, Iran. 8. College of Animal Science and Technology, 12469Northwest A&F University, Yangling, Xianyang, China. 9. 199675Wake Forest Institute for Regenerative Medicine, School of Medicine, Wake Forest University, Winston-Salem, NC, USA. 10. Department of Pharmacology, 48432Tabriz University of Medical Sciences, East Azerbaijan Province, Tabriz, Iran.
Abstract
OBJECTIVES: Our work was aimed at investigating the impact and regulatory mechanism of coenzyme Q10 (CoQ10) on exogenous scrotal heat stress (HS)-induced testicular injuries in rats. METHODS: The rats (n = 32) were assigned into four groups: control, HS control, HS+CoQ10, and CoQ10. To induce HS, rats' testicles were immersed in a water bath at 43°C for 20 min, every other day for 8 weeks. Moreover, treatment with CoQ10 (10 mg/kg) immediately started before inducing HS and continued for 8 weeks. KEY FINDINGS: HS decreased the activity of the testicular antioxidant system, superoxide dismutase, glutathione peroxidase, and catalase, while the amount of lipid peroxidation (malondialdehyde) was increased. The index of apoptosis and mRNA expression of caspase 3 and Bax were increased, while the mRNA expression levels of Bcl-2, 3β-HSD, and 17β-HSD3 decreased after HS. Exposure to HS decreased the serum testosterone level but increased the activation of pro-inflammatory cytokines (interleukin 1 beta and tumor necrosis factor-alpha). Deleterious effects of HS on the mentioned parameters were reduced when the rats were treated with CoQ10. CONCLUSIONS: CoQ10 could suppress the degenerative effects following testicular hyperthermia via its antiapoptotic, anti-inflammation, antioxidative, and androgen synthesis effects.
OBJECTIVES: Our work was aimed at investigating the impact and regulatory mechanism of coenzyme Q10 (CoQ10) on exogenous scrotal heat stress (HS)-induced testicular injuries in rats. METHODS: The rats (n = 32) were assigned into four groups: control, HS control, HS+CoQ10, and CoQ10. To induce HS, rats' testicles were immersed in a water bath at 43°C for 20 min, every other day for 8 weeks. Moreover, treatment with CoQ10 (10 mg/kg) immediately started before inducing HS and continued for 8 weeks. KEY FINDINGS: HS decreased the activity of the testicular antioxidant system, superoxide dismutase, glutathione peroxidase, and catalase, while the amount of lipid peroxidation (malondialdehyde) was increased. The index of apoptosis and mRNA expression of caspase 3 and Bax were increased, while the mRNA expression levels of Bcl-2, 3β-HSD, and 17β-HSD3 decreased after HS. Exposure to HS decreased the serum testosterone level but increased the activation of pro-inflammatory cytokines (interleukin 1 beta and tumor necrosis factor-alpha). Deleterious effects of HS on the mentioned parameters were reduced when the rats were treated with CoQ10. CONCLUSIONS: CoQ10 could suppress the degenerative effects following testicular hyperthermia via its antiapoptotic, anti-inflammation, antioxidative, and androgen synthesis effects.
Authors: Hossam R El-Sherbiny; Elshymaa A Abdelnaby; K H El-Shahat; Noha Y Salem; Eman S Ramadan; Shimaa G Yehia; Mohamed Fathi Journal: Vet Res Commun Date: 2022-09-01 Impact factor: 2.816