Jasper Stevens1, Mireille A Wessels1, Jan Roggeveld1, Remco A Koster1,2, Claire Cj Dekkers1, Maaike K van Gelder3, Ron T Gansevoort4, Hiddo Jl Heerspink1, Daan J Touw1,5. 1. Department of Clinical Pharmacy & Pharmacology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. 2. Bioanalytical Laboratory, PRA Health Sciences, Assen, The Netherlands. 3. Division of Internal Medicine & Dermatology, Nephrology & Hypertension, University Medical Center Utrecht, Utrecht, The Netherlands. 4. Department of Internal Medicine, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. 5. Department of Pharmaceutical Analysis, Groningen Research Institute of Pharmacy, University of Groningen, Groningen, The Netherlands.
Abstract
Aim: Iohexol plasma clearance is used as an indicator of kidney function in clinical and preclinical settings. To investigate the pharmacokinetic profile of iohexol, a rapid, simple method for measurement of iohexol in different matrices and species was needed. Materials & methods: Iohexol was separated on an Accucore C18 column (Thermo Fisher Scientific, CA, USA). Detection was performed on a Thermo Scientific Quantiva tandem quadrupole mass spectrometer. The method was validated according to the requirements for bioanalytical methods issued by the US FDA and European Medicines Agency. Conclusion: We developed and validated a fast and efficient analytical method, suitable for analyzing iohexol in human EDTA plasma, human lithium-heparin plasma, human urine and goat- and pig EDTA plasma, using only one calibration line prepared in human EDTA plasma.
Aim: Iohexol plasma clearance is used as an indicator of kidney function in clinical and preclinical settings. To investigate the pharmacokinetic profile of iohexol, a rapid, simple method for measurement of iohexol in different matrices and species was needed. Materials & methods: Iohexol was separated on an Accucore C18 column (Thermo Fisher Scientific, CA, USA). Detection was performed on a Thermo Scientific Quantiva tandem quadrupole mass spectrometer. The method was validated according to the requirements for bioanalytical methods issued by the US FDA and European Medicines Agency. Conclusion: We developed and validated a fast and efficient analytical method, suitable for analyzing iohexol in humanEDTA plasma, humanlithium-heparin plasma, human urine and goat- and pigEDTA plasma, using only one calibration line prepared in humanEDTA plasma.
Entities:
Keywords:
LC–MS/MS; goat plasma; human plasma; human urine; iohexol; pig plasma
Authors: Joost C de Vries; Maaike K van Gelder; Anneke S Monninkhof; Sabbir Ahmed; Diënty H M Hazenbrink; Tri Q Nguyen; Gèrard A P de Kort; Evert-Jan P A Vonken; Koen R D Vaessen; Jaap A Joles; Marianne C Verhaar; Karin G F Gerritsen Journal: Toxins (Basel) Date: 2022-09-14 Impact factor: 5.075
Authors: Maaike K van Gelder; Jasper Stevens; Tobias T Pieters; Koen R D Vaessen; Jaap A Joles; Marianne C Verhaar; Karin G F Gerritsen Journal: Biology (Basel) Date: 2021-05-23