Dan He1, Xiaowei Zhang1,2, Song Gao1,3, Hailong You1,4, Yanbing Zhao1, Li Wang5. 1. Department of Pathogenobiology, Jilin University Mycology Research Center, College of Basic Medical Sciences, Jilin University, No. 126 Xinmin Street, Changchun, 130021, Jilin, China. 2. The Laboratory of Changchun Children's Hospital, Changchun, 130061, China. 3. Beijing ZhongKai TianCheng Bio-Technology Co. Ltd, Beijing, 101111, China. 4. Department of Pediatrics, The First Affiliated Hospital, Jilin University, Changchun, 130021, China. 5. Department of Pathogenobiology, Jilin University Mycology Research Center, College of Basic Medical Sciences, Jilin University, No. 126 Xinmin Street, Changchun, 130021, Jilin, China. wli99@jlu.edu.cn.
Abstract
PURPOSE: Sporothrix schenckii is a thermally dimorphic fungus. In a saprotrophic environment or culturing at 25 °C, it grows as mycelia, whereas in host tissues or culturing at 37 °C, it undergoes dimorphic transition and division into pathogenic yeast cells. S. schenckii can cause serious disseminated sporotrichosis in immunocompromised hosts and presents an emerging global health problem. The mycelium-to-yeast transition was a consequence of the adaptive process to different environment. Some studies showed that the transition was significantly related to the virulence and pathogenesis of dimorphic fungi. However the genetic mechanisms of this complicated biological process are poorly understood. METHOD: Our study presented a comparative transcriptomic analysis perspective on temperature stress in a visceral isolates of S. schenckii, obtaining more genetic information related to dimorphic transition. RESULTS: The 9.38 Gbp dataset was generated and assembled into 14,423 unigenes. Compared with gene and protein databases, 9561 unigenes were annotated. Comparative analysis identified 1259 genes expressed differentially in mycelium and yeast phase, and were categorized into a number of important biological processes, such as synthesis and metabolism, transmembrane transport, biocatalysis, oxidation reduction, and cellular signal transduction. CONCLUSIONS: The findings suggested that temperature-dependent transition was tightly associated with stress adaptation, growth and development, signal regulation, adhesion, and colonization, which was predicted to be related with virulence and pathogenesis. Collection of these data should offer fine-scale insights into the mechanisms of dimorphism and pathogenesis of S. schenckii, and meanwhile facilitate the evolutionary and function studies of other dimorphic fungi.
PURPOSE:Sporothrix schenckii is a thermally dimorphic fungus. In a saprotrophic environment or culturing at 25 °C, it grows as mycelia, whereas in host tissues or culturing at 37 °C, it undergoes dimorphic transition and division into pathogenic yeast cells. S. schenckii can cause serious disseminated sporotrichosis in immunocompromised hosts and presents an emerging global health problem. The mycelium-to-yeast transition was a consequence of the adaptive process to different environment. Some studies showed that the transition was significantly related to the virulence and pathogenesis of dimorphic fungi. However the genetic mechanisms of this complicated biological process are poorly understood. METHOD: Our study presented a comparative transcriptomic analysis perspective on temperature stress in a visceral isolates of S. schenckii, obtaining more genetic information related to dimorphic transition. RESULTS: The 9.38 Gbp dataset was generated and assembled into 14,423 unigenes. Compared with gene and protein databases, 9561 unigenes were annotated. Comparative analysis identified 1259 genes expressed differentially in mycelium and yeast phase, and were categorized into a number of important biological processes, such as synthesis and metabolism, transmembrane transport, biocatalysis, oxidation reduction, and cellular signal transduction. CONCLUSIONS: The findings suggested that temperature-dependent transition was tightly associated with stress adaptation, growth and development, signal regulation, adhesion, and colonization, which was predicted to be related with virulence and pathogenesis. Collection of these data should offer fine-scale insights into the mechanisms of dimorphism and pathogenesis of S. schenckii, and meanwhile facilitate the evolutionary and function studies of other dimorphic fungi.
Authors: Vincent M Bruno; Zhong Wang; Sadie L Marjani; Ghia M Euskirchen; Jeffrey Martin; Gavin Sherlock; Michael Snyder Journal: Genome Res Date: 2010-09-01 Impact factor: 9.043
Authors: Arunaloke Chakrabarti; Alexandro Bonifaz; Maria Clara Gutierrez-Galhardo; Takashi Mochizuki; Shanshan Li Journal: Med Mycol Date: 2014-12-19 Impact factor: 4.076
Authors: Christina A Cuomo; Nuri Rodriguez-Del Valle; Lizaida Perez-Sanchez; Amr Abouelleil; Jonathan Goldberg; Sarah Young; Qiandong Zeng; Bruce W Birren Journal: Genome Announc Date: 2014-05-22