Multifunctional N-P-doped carbon dots for regulation of apoptosis and autophagy in B16F10 melanoma cancer cells and in vitro imaging applications.

Rationale: The present study reports the multifunctional anticancer activity against B16F10 melanoma cancer cells and the bioimaging ability of fluorescent nitrogen-phosphorous-doped carbon dots (NPCDs).
Methods: The NPCDs were synthesized using a single-step, thermal treatment and were characterized by TEM, XPS, fluorescence and UV-Vis spectroscopy, and FTIR analysis. The anticancer efficacy of NPCDs was confirmed by using cell viability assay, morphological evaluation, fluorescent live-dead cell assay, mitochondrial potential assay, ROS production, RT-PCR, western-blot analysis, siRNA transfection, and cellular bioimaging ability.
Results: The NPCDs inhibited the proliferation of B16F10 melanoma cancer cells after 24 h of treatment and induced apoptosis, as confirmed by the presence of fragmented nuclei, reduced mitochondrial membrane potential, and elevated levels of reactive oxygen species. The NPCDs treatment further elevated the levels of pro-apoptotic factors and down-regulated the level of Bcl2 (B-cell lymphoma 2) that weakened the mitochondrial membrane, and activated proteases such as caspases. Treatment with NPCDs also resulted in dose-dependent cell cycle arrest, as indicated by reduced cyclin-dependent kinase (CDK)-2, -4, and -6 protein levels and an enhanced level of p21. More importantly, the NPCDs induced the activation of autophagy by upregulating the protein expression levels of LC3-II and ATG-5 (autophagy-related-5) and by downregulating p62 level, validated by knockdown of ATG-5. Additionally, owing to their excellent luminescence property, these NPCDs were also applicable in cellular bioimaging, as evidenced by the microscopic fluorescence imaging of B16F10 melanoma cells.
Conclusion: Based on these findings, we conclude that our newly synthesized NPCDs induced cell cycle arrest, autophagy, and apoptosis in B16F10 melanoma cells and presented good cellular bioimaging capability. © The author(s).

Introduction

n class="Disease">Melanoma is a highly resistaclass="Chemical">nt aclass="Chemical">nd class="Chemical">n class="Disease">malignant cancer accounting for around 3% of all malignant tumor cases, and its incidence has increased faster than any other cancer type during recent decades. Despite accounting for only 4% of all skin cancer cases, it is the most aggressive and causes approximately 80% of all deaths from skin cancer 1. If diagnosed early in the course of the disease, melanoma is readily cured by simple wide surgical excision 1. However, once it metastasizes, there is presently no treatment that reliably affects the course of the disease. Hence, there is a medical necessity for new nanodrugs that have the potential of inducing apoptosis in cancer cells but with no adverse side effects in healthy cells 2.

There are various pathways for class="Disease">cancer cell death, such as apoptosis, autophagy, or class="Chemical">n class="Disease">necrosis. Apoptosis is programmed cell death during which reactive oxygen species (ROS) are free radicals generated in response to external stimuli 3. The generated ROS causes stress on the mitochondria, leading to dysregulation of the mitochondrial membrane and protein markers, and the released cytochrome C causes activation of proteases such as caspases, which cause apoptotic cell death 3-5.

In addition to apoptosis, man class="Chemical">croautophagy (autophagy) is a well-kclass="Chemical">nowclass="Chemical">n coclass="Chemical">nserved orchestrated process for removiclass="Chemical">ng damaged cellular orgaclass="Chemical">nelles or uclass="Chemical">nused metabolites 6. Moreover, autophagy is a lysosomal catabolic process that is very well kclass="Chemical">nowclass="Chemical">n for its dual fuclass="Chemical">nctioclass="Chemical">n as cell survival aclass="Chemical">nd cell death. Duriclass="Chemical">ng starvatioclass="Chemical">n, the autophagic process provides biofuel to damaged orgaclass="Chemical">nelles, which further affects cell survival or cell death iclass="Chemical">n a coclass="Chemical">ntext-depeclass="Chemical">ndeclass="Chemical">nt maclass="Chemical">nclass="Chemical">ner. The autophagic process caclass="Chemical">n be activated by exterclass="Chemical">nal stimuli, which also iclass="Chemical">nduces caspase-iclass="Chemical">ndepeclass="Chemical">ndeclass="Chemical">nt cell death 6. Furthermore, class="Chemical">n class="Disease">necrosis is characterized morphologically by widespread swelling of cell membranes, often escorted by chromatin condensation and an uneven DNA degradation pattern. The release of the intracellular contents leads to enormous cellular damage that affects neighboring cells, which elucidates why necrosis triggers inflammatory and autoimmune reactions 7. Several treatment strategies have been applied to treat a variety of cancers, but they have shown less effectiveness. Therefore, it is essential to find nanodrugs as alternatives to chemical drugs to eradicate cancer cells 8.

Considering similar applications, presently, the fluorescence imaging of cells, biological tissues, and specific localized sub-cellular organelles (nuclei, mitochondria, and/or lysosomes) based on fluorescent molecules have attracted considerable attention acn class="Chemical">ross medical fields for aclass="Chemical">n iclass="Chemical">n-depth uclass="Chemical">nderstaclass="Chemical">ndiclass="Chemical">ng of biological processes aclass="Chemical">nd pathways toward early disease diagclass="Chemical">nosis aclass="Chemical">nd drug developmeclass="Chemical">nt iclass="Chemical">n class="Chemical">n class="Chemical">biomedicine 9-11. Besides, fluorescence imaging can visualize the expression and activity of specific molecules as well as cellular and biological processes that influence tumor behavior and response to therapeutic drugs 12. For example, the nucleolus is the key site in the nucleus that synthesizes, processes, and assembles the ribosomal ribonucleic acids 13, and its function is closely related to cell growth and proliferation 13. Enlarged or aberrant nucleoli and their associated number are often indicative of types of cancer and other human disorders 13. In the case of mitochondria and lysosomes, permeabilization of their constitutes is one of the major checkpoints in inducing apoptotic and necrotic cell death; both of them play pivotal roles in cell life and death 13.

For the imaging purposes, multi-emissive n class="Chemical">carbon dots (class="Chemical">n class="Chemical">CDs) 13, is a well-known example of nanotechnology, that have attracted significant attention in the field of the imaging applications due to their unique core structure with rich surface functional species 14. Now, the optical and electronic properties of CD can be enhanced via the process of doping 15-17, that can either in the form of metal ions and heteroatom (e.g. with N, B, P etc.), individually 18, 19 or multiple 20-22 and sometimes its self-doping 23, 24. Doping of N and P considerably improves the chemical activity and physicochemical properties of CDs due to the generation of the new surficial active sites in terms of surface defects that can improve their optical properties 25. Along with these, they also possess excellent photochemical stability, resistance to photo-bleaching, and excellent biocompatibility. That makes them useful in various other fields as photocatalysis 26, sensing 18, 24, electrochemistry 27, and biological imaging 28-31.

In this article, a simple high-yield synthetic protocol for the fabrications of multi-heteroatom (n class="Chemical">N-P) class="Chemical">n class="Chemical">co-doped CDs (NPCDs) using a single-step, thermal treatment is reported, where imidazole is used as the N source, phosphoric acid as the P source, and polyethylene glycol (PEG) as the carbon precursor. The as-synthesized NPCDs showed a good value of quantum yield ~9%. Also, we explored the underlying mechanism of the NPCDs' anticancer potential against B16F10 melanoma cells and their cellular bioimaging application in vitro.

Materials and Methods

Chemicals and reagents

The chemical reagents such as n class="Chemical">PEG, class="Chemical">n class="Chemical">ammonia solution, imidazole, and phosphoric acid were procured from Sigma-Aldrich, India. Reagents for microscopy analysis like osmium tetroxide, glutaraldehyde, and ethanol were procured from Duksan Chemicals (Gyeonggi-do, South Korea). All other chemicals used were of analytical grade and supplied by Sigma-Aldrich (St. Louis, MO, USA), and all the synthesis experiments were executed in deionized (DI) water.

Preparation of the NPCDs

The preparation of n class="Chemical">NPCDs was doclass="Chemical">ne by the heaticlass="Chemical">ng process of reageclass="Chemical">nts 3 g class="Chemical">n class="Chemical">imidazole (N source), 10 mL PEG (carbon source), and 10 mL phosphoric acid (P source) in a two neck round bottom flask by maintaining the temperature at 150 °C for 60 min. 28, 32, 33 The excess of phosphoric acid was removed by the addition of ammonia solution in the as-prepared brown solution followed by the centrifugation process (30 min at 6,000 rpm, three repetitive steps) and dried resultant supernatant for removal of excess water. Afterward, the produced solution was termed as NPCDs from Scheme 1. The QY of NPCDs was ~ 9 % (slightly different for different batches) at 348 nm wavelength, calculated by the given formula:

Scheme 1

Schematic representation showing the synthesis of nitrogen-phosphorous-doped carbon dots (NPCDs).

Where QY is represented as Q, the measured integrated emission intensity as I, refractive index of the solvent as η and A is the optical density. The subsn class="Chemical">cript “R” refers to the refereclass="Chemical">nce staclass="Chemical">ndard of class="Chemical">n class="Species">quinine sulphate with known QY 28, 34, 35.

Instrumentation used for the characterization of NPCDs

n class="Chemical">NPCDs examiclass="Chemical">natioclass="Chemical">n: Traclass="Chemical">nsmissioclass="Chemical">n electroclass="Chemical">n micclass="Chemical">n class="Chemical">roscopy (TEM; JEM-2100F, JEOL, Japan) at 200 kV was used for internal morphology analysis. X-ray photoelectron spectroscopy (XPS) was applied for dopant and surficial group analysis using ESCA system (Omicron Nanotechnology, Oxford Instruments, Malaviya National Institute of Technology Jaipur (MNIT), India). Fluorescence measurements were done using a Fluoromax 4C-L system (Horiba Scientific, NJ, USA and Fluoromax 4C.L. Spectrophotometer, MNIT, India). Jasco V-730 spectrophotometer, MNIT, India recorded ultraviolet-visible (UV-Vis) spectra, whereas a Bruker Fourier-transform infrared (FTIR) spectrometer (Vertex 70 model, MNIT, India) was used for the functional group analysis.

Cell lines

n class="CellLine">B16F10 class="Chemical">n class="Disease">melanoma cells, colon cancer cells (HCT 116 and HT29), lung cancer cells (A549) and cervical cancer cells (HeLa), BEAS-2B human lung epithelial cells, and HACAT human keratinocytes were purchased from the American Type Culture Collection (Manassas, VA, USA). These cells were cultured in RPMI-1640 (Invitrogen, Carlsbad, CA, USA) media, supplemented with 10% (v/v) fetal calf serum (Invitrogen, Carlsbad, CA, USA) and a 1% penicillin-streptomycin cocktail at 37 °C in a 5% CO2 incubator 36.

Cell viability, cytotoxicity and morphological evaluation

The cytotoxic effect of n class="Chemical">NPCDs was first determiclass="Chemical">ned by class="Chemical">n class="Chemical">3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay 37. Briefly, 5×104 cells/well of various cancer cells such as B16F10 melanoma cells, colon cancer cells (HCT 116 and HT29), lung cancer cells (A549) and cervical cancer cells (HeLa), BEAS-2B human lung epithelial cells, HACAT human keratinocytes were seeded and after attachment further incubated for 24 h in the presence of various concentrations of NPCDs (0-120 μL/mL) at 37 °C in a CO2 incubator. After incubation, the cells were treated with MTT solution (5 mg/mL), yielding dark-blue-colored formazan crystals, which were dissolved in 50 μL dimethyl sulfoxide (DMSO). The absorbance (540 nm) of these crystals was measured using a microplate reader (Bio-Tek instrument Co., WA, USA).

n class="Disease">Cytotoxicity was further coclass="Chemical">nfirmed by the lactate dehydrogeclass="Chemical">nase (LDH) assay. Briefly, cells were treated with various coclass="Chemical">nceclass="Chemical">ntratioclass="Chemical">ns of class="Chemical">n class="Chemical">NPCDs for 24 h at 37 °C. After incubation, media were removed and processed to evaluate extracellular LDH release using an LDH detection kit (Sigma-Aldrich, St. Louis, MO, USA) as per the manufacturer's guidelines. Cytotoxicity was evaluated by calculating the absorbance at 490 and 690 nm.

In addition, a micclass="Chemical">roscopic examiclass="Chemical">natioclass="Chemical">n was carried out to determiclass="Chemical">ne the morphological chaclass="Chemical">nges iclass="Chemical">n class="Chemical">n class="CellLine">B16F10 cells after exposure to NPCDs using an inverted microscope (Nikon Eclipse TS200, Nikon Corp., Tokyo, Japan).

Cells exhibiting apoptotic characteristics are sensitive to n class="Chemical">formamide. Therefore, declass="Chemical">natured Dclass="Chemical">n class="Chemical">NA was detected using a monoclonal antibody against single-stranded DNA with an ApoStrand™ ELISA (enzyme-linked immunosorbent assay) apoptosis detection kit (Enzo Life Sciences, Plymouth Meeting, PA, USA) in accordance with the manufacturer's instructions.

During the clonogenic assay, cells were seeded in a 12-well culture plate, allowed to attach for 24 h, and then subjected to treatment with n class="Chemical">NPCDs at differeclass="Chemical">nt coclass="Chemical">nceclass="Chemical">ntratioclass="Chemical">ns. After seveclass="Chemical">n days of iclass="Chemical">ncubatioclass="Chemical">n at 37 °C iclass="Chemical">n a class="Chemical">n class="Chemical">CO2 incubator, cells were washed with phosphate buffer saline (PBS) and stained with 0.5% crystal violet 38.

Scanning electron microscopy (SEM) and Bio-transmission electron microscopy (bio-TEM) studies

Observing NPCDs-induced B16F10 morphological changes

For SEM, in brief, cells were grown on coverslips, washed with 1× class="Chemical">PBS, aclass="Chemical">nd fixed with 2.5% class="Chemical">n class="Chemical">glutaraldehyde, followed by dehydration using gradient ethanol. The coverslips were then dried in a desiccator, placed on the SEM stage, super-coated with gold, and subsequently visualized under SEM.

To observe the apoptosis morphology of n class="CellLine">B16F10 cells after traclass="Chemical">nsfectioclass="Chemical">n with class="Chemical">naclass="Chemical">no-complexes, the class="Chemical">n class="Gene">NPCD-treated B16F10 cells were collected and fixed, then frozen and made into sections. The cell sections were stuck on the copper online surface and observed with TEM.

Fluorescence staining

Apoptotic and live-dead morphological evaluation

n class="Chemical">Nuclear fragmeclass="Chemical">ntatioclass="Chemical">n as a marker of apoptosis was evaluated by meaclass="Chemical">ns of class="Chemical">n class="Chemical">Hoechst-propidium iodide (Hoechst-PI) staining. Briefly, cells (5×104 cells/well) were seeded in a 24-well plate and allowed to attach for 24 h. The cells were then subjected to 24-h treatment with NPCDs (0-30 μL/mL). After incubation, the cells were stained with Hoechst-PI (1 mg/mL) and images were captured with epifluorescence imaging on an inverted microscope (Nikon Eclipse TS200, Nikon Corp., Japan). After the compound treatment, the cells were stained with Live/Dead Cell Viability Assay kit reagents (Thermo Fisher Scientific, Waltham, MA, USA) and images were captured under the aforementioned fluorescence microscope. The number of apoptotic cells and cell index were calculated as percent apoptotic nuclei compared with the total number of cells 36.

Oxidative stress staining

Intracellular n class="Chemical">ROS productioclass="Chemical">n iclass="Chemical">n respoclass="Chemical">nse to class="Chemical">n class="Gene">NPCD exposure of the B16F10 cells was determined using 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) fluorescence stain. Briefly, cells (5×104 cells/well) were seeded in a 24-well culture plate, allowed to attach for 24 h, and then subjected to treatment at different concentrations (0-30 μL/mL) of NPCDs. Afterward, the cells were washed with PBS and subsequently incubated with 20 mM H2DCFDA dye for 30 min 37. The cells were washed twice with PBS and ROS formation was immediately detected via epifluorescence imaging. Fluorescence intensity was quantified by using the ImageJ software (Maryland, USA).

Mitochondrial membrane potential (∆ψm)

As previously mentioned, n class="CellLine">B16F10 cells were growclass="Chemical">n iclass="Chemical">n a 24-well culture plate aclass="Chemical">nd treated with various coclass="Chemical">nceclass="Chemical">ntratioclass="Chemical">ns of class="Chemical">n class="Chemical">NPCDs (0-30 μL/mL). After 24 h of incubation, the cells were washed with PBS and stained with Rhodamine-123 (1 μg/mL) for 30 min. Epifluorescence images of the cells were obtained via an inverted microscope (Nikon Eclipse TS200, Nikon Corp., Japan) 4.

Western blot analysis

The n class="CellLine">B16F10 class="Chemical">n class="Disease">melanoma cancer cells were cultured in a 3 mL culture dish and then subjected to 24-h NPCDs (0-30 μL/mL) treatment. Furthermore, the cells were washed with PBS, lysed using a radio-immunoprecipitation assay lysis buffer, and centrifuged at 12,000 rpm for 15 min at 4 °C. The obtained supernatant was used for Western blotting after protein quantification. The protein sample (25 μg) was subjected to a 12% SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and then transferred onto a polyvinylidene fluoride (PVDF) membrane, with which 5% skimmed milk was used for membrane blocking. This was incubated with specific antibodies overnight at 4 °C and further visualized using enhanced Western blot chemiluminescence detection reagents (Amersham Biosciences Inc., Piscataway, NJ, USA). Primary and secondary antibodies used in the study are presented in Table S1.

Detection and quantification of autophagic vacuole formation

Briefly, n class="CellLine">B16F10 cells (1×104 cells/well) were seeded iclass="Chemical">n 24-well plates aclass="Chemical">nd allowed to adhere overclass="Chemical">night. After treatmeclass="Chemical">nt with differeclass="Chemical">nt coclass="Chemical">nceclass="Chemical">ntratioclass="Chemical">ns of class="Chemical">n class="Chemical">NPCDs (0-30 μL/mL) for 24 h, autophagic vacuoles were detected with the help of acridine orange (AO) staining (100 μg/mL). Fluorescence images were captured under the inverted microscope at 40× magnification. Moreover, lysosomal activity was evaluated with the LysoTracker VR Red autophagy detection kit (Enzo Life Sciences, Plymouth Meeting, PA, USA) according to the manufacturer's instructions. DAPI (4′,6-diamidino-2-phenylindole) was used as a counterstain for the nuclei. Fluorescence images were captured under the inverted microscope and the mean fluorescence intensity was quantified using the ImageJ software.

siRNA transfection studies

The n class="Species">human class="Chemical">n class="Gene">Atg5 siRNA (5′-ACGCUAAAAGGCUUACAGUAUCAGA-3′) was synthesized by integrated DNA technology (Iowa, USA). Non-specific control siRNA duplexes (Integrated DNA Technologies, Iowa, USA) were also used in parallel experiment. For the experiment, before transfection, 1 × 105 B16F10 melanoma cells were seeded in 6-well plates and grown in 3 mL of RPMI-1640 supplemented with 10% fetal bovine serum and antibiotics. The siRNA or control duplexes were transfected into cells with trans-IT-TKO transfection reagent and incubated for 48 h. Next, cells were harvested for RNA isolation and evaluated for the expression of atg5.

Isolation of RNA and quantitative real-time PCR (qRT-PCR)

Total Rn class="Chemical">NA was isolated from class="Chemical">n class="CellLine">B16F10 cells using an RNA-spinTM extraction kit (Intron Biotechnology, Korea) as per the manufacturer's instructions and quantified by a Qubit22s 2.0 fluorometer RNA assay kit (Life technologies, USA). cDNA synthesis was carried out using a Maxime RT Premix cDNA synthesis kit (Intron Biotechnology, Korea) according to the manufacturer's instructions. Finally, qRT-PCR was performed using an Agilent technology qPCR system (CA USA). Details of the primers used in the study are given in Table S2.

Cellular bioimaging of B16F10 cells

We report highly fluorescent n class="Chemical">NPCDs suitable for biological labeliclass="Chemical">ng aclass="Chemical">nd imagiclass="Chemical">ng applicatioclass="Chemical">ns. For liviclass="Chemical">ng cell staiclass="Chemical">niclass="Chemical">ng experimeclass="Chemical">nts, class="Chemical">n class="CellLine">B16F10 melanoma cells cultured initially were seeded into 24-well plates and incubated at 37 °C in a 5% CO2 environment for 12-24 h. Subsequently, NPCDs (0-10 μL/mL) were injected into each treatment well (non-treated wells served as the control), followed by incubation for 30 min. The cells were washed twice or thrice with PBS after incubation. Epifluorescence images of the cells with/without NPCDs were obtained under an inverted microscope (ex: green 500-570 nm; red 610-750 nm, and blue 450-500 nm; Nikon Eclipse TS200, Nikon Corp., Tokyo, Japan). Each experiment was repeated three times (independently), and at least three different fields were observed for each culture 38.

Statistical analysis

All experiments were performed in triplicate. The results are expressed as the mean ± standard deviation of three independent experiments. Statistical significance was calculated using Student's t-test (*p < 0.05, **p <0.01, *** p < 0.001).

Results

Characterization

A merge graph of absorption and fluorescence spectra of n class="Chemical">NPCDs is showclass="Chemical">n iclass="Chemical">n Figure 1A. The absorptioclass="Chemical">n spectra exhibited two baclass="Chemical">nds at ~275 class="Chemical">nm (π-π*) aclass="Chemical">nd ~320 class="Chemical">nm (class="Chemical">n-π*) due to the emergeclass="Chemical">nce of C=C aclass="Chemical">nd C=class="Chemical">n class="Chemical">N/O/P groups, respectively 20, 39, 40. The fluorescence spectra of NPCDs showed excitation wavelength-dependent spectra (340-520 nm) with emissions in the blue-green region (Max. = ~ 425 nm) 41. The core structure and morphology of NPCDs suggested the spherical shape of particle with an average diameter of 6-8 nm, as observed from the TEM analysis (Figure 1B and inset). The HRTEM image of the homogeneously distributed NPCDs are shown by the yellow highlighted circles (Figure 1C) and displayed the graphitic pattern and interplanar spacing of 0.23 nm (Figure 1D). Further, the surface functional groups and doping of each atom were characterized by the XPS and FTIR spectroscopy, respectively. The full survey scan of NPCDs showed the presence of different atoms as a peak at ~285.02 eV for carbon (67.58 %), at ~531.8 eV for oxygen (17.09 %), at ~399.9 eV for nitrogen (5.63 %), and at ~190.1 and ~133.5 eV for phosphorus (9.7 %) from C1s, O1s, N1s, P2s and P2p, respectively (Figure 2A). Figure 2B displays C1s short-scan spectra that were exhibiting five different bindings as a peak at ~283.07 eV, which corresponds to the binding C=C, ~284.9 eV for C-C, ~285.8 eV for C-O/N/P, ~286.8 eV for C=N, and ~289.2 eV for C=O binding 42. The different types of oxygen bindings were observed in O1s short scan spectra such as O-N/P at 530.7 eV, C-O at 532.6 eV, and C=O at 533.5eV (Figure 2C). N1s spectra were showing three different bindings of O=N-C at 398.8 eV, C-N at 400.0 eV, and C=N 400.8 eV confirming the doping of N element (Figure 2D). P2p short scan spectra showing two different types of binding as P-O and P-C bonds at ~133.8 eV and ~133 eV (Figure 2E), confirmed the doping of P atom 39. Moreover, the FTIR spectrum showing the functional groups O/N-H, C-H, C=O/C=C, C=N, C-O, and P-O-C vibrations occurred at peak position ~3360/3155 cm-1, ~2854 cm-1, ~1735-1581 cm-1, ~1440.5 cm-1, ~1292 cm-1, ~1092-820 cm-1 wavenumber, respectively (Figure 2F) 28, 29.

Figure 1

Optical and morphological characterization of nitrogen-phosphorous-doped carbon dots (NPCDs). (A) UV-visible and fluorescence emission spectra, (B) a low-resolution transmission electron microscopy (TEM) image with size histogram in inset image, (C and D) high-resolution TEM images.

Figure 2

Structural characterization of nitrogen-phosphorous-doped carbon dots (NPCDs). (A) Full survey XPS scan with elemental composition; the corresponding deconvoluted spectra of (B) C1s, (C) O1s, (D) N1s, and (E) P2p short scans; and (F) FTIR spectra of NPCDs.

Anticancer effects of the NPCDs

NPCDs promote cytotoxicity in B16F10 cells

Primarily, we performed n class="Chemical">MTT assay with various class="Chemical">n class="Disease">cancer cell lines in the presence of NPCDs (0-120 µL/mL) for 24 h, and the values obtained from MTT assay results were utilized to construct the heatmap as depicted in Figure S1, and based on the best results obtained, we further selected melanoma cancer cell (B16F10) in subsequent assays for rest of the study. The MTT assay revealed that 30.01 μL/mL of NPCDs served as the fifty percent inhibitory concentration (IC50) against B16F10 cells (Figure S2). The MTT assay (Figure 3A) and LDH (Figure 3B) assays proved the dose-dependent anticancer potential of NPCDs against B16F10 cells (Figure 3). Furthermore, NPCDs did not show any serious cytotoxic effect on both the tested normal cells (human keratinocytes HACAT and BEAS-2B lung epithelial cells) (Figure S3). Moreover, we determined whether NPCD-induced DNA fragmentation, which is characteristic of apoptosis, or the lack thereof by performing the ApoStrand ELISA assay. The results of this assay confirmed distortion in the chromatin structure, which is associated with apoptosis (Figure 3C). The dose-dependent effect of NPCDs on cell morphology is depicted in Figure 3D, which showed distortion and killing of the NPCDs-treated cells, and rupturing of cell membrane. The cytotoxicity was further validated via clonogenic assay, which confirmed reduced colony formation after treatment with NPCDs (Figure 3E). Additional validation was obtained through SEM analysis, which revealed the distorted morphology of the B16F10 cell at an NPCD concentration of 30 µL/mL compared with that of the control group with intact cell morphology (Figure 4A). Moreover, the results of a bio-TEM analysis showed broken vacuoles and decreased numbers of mitochondria, lysosomes, late lysosomes, vacuoles, ribosomes as well as autophagosome formation (Figure 4B). These results collectively suggest the cytotoxic activity of the NPCDs against the B16F10 cells.

Figure 3

Cytotoxic potential of nitrogen-/phosphorous-doped carbon dots (NPCDs) against B16F10 melanoma cells. (A and B) MTT and LDH assay, respectively of the NPCDs (0-30 μL/mL)-treated cells after 24 h of incubation. (C) Apoptosis assay after treatment with NPCDs measured at A405 (D) Morphological changes in B16F10 cells after treatment with NPCDs (0-30 μL/mL). Images were captured at 20× magnification [scale bar =0.1 mm]. (E) Clonogenic assay where B16F10 cells were cultured in the presence and absence of NPCDs over 7 days, and then subjected to crystal violet staining. Each value in the bar graph represents the mean ±SD of three independent experiments. Values with different superscripts differ significantly from each other (P < 0.05).

Figure 4

Evaluation of the morphological and internal cellular damage to B16F10 melanoma cancer cells after treatment with nitrogen-/phosphorous-doped carbon dots (NPCDs) via scanning electron microscopy (SEM) and bio- transmission electron microscopy (bio-TEM) analysis. (A) SEM images of the (i) control and (ii) 5 μL/mL, (iii) 15 μL/mL and (iv) 30 μL/mL NPCD-treated B16F10 cells; and (B) Bio-TEM images of the (i) control and (ii) 30 μL/mL NPCD-treated B16F10 cells. L, lysosome; LE, late lysosome; V, vacuole; MI, mitochondrion; and R: ribosome (Scale: 1 μm).

NPCDs mediate apoptotic morphology in B16F10 cells

The ability of the n class="Chemical">NPCDs to iclass="Chemical">nduce apoptosis aclass="Chemical">nd the stages thereof was determiclass="Chemical">ned with the help of dual class="Chemical">n class="Chemical">AO/ethidium bromide staining, with which the normal cells and the apoptotic cells appear green and orange (or red), respectively. The results revealed that the NPCDs caused a dose-dependent increase in the number of apoptotic cells. For example, the apoptotic index of the cells treated with 30 μL/mL NPCDs was significantly (p < 0.05), i.e. 7.5-fold higher than that of the control (Fig 5A). We validated the results via Hoechst- PI staining, which indicates the enhancement of dead and apoptotic cells, and the elevated red fluorescence depicted DNA damage and chromatin condensation in a dose-dependent manner. The number of apoptotic cells among those treated with 30 μL/mL NPCDs was significantly higher (p < 0.05; 5.6-fold) than those in the control (Figure 5B). Furthermore, the NPCD-induced apoptosis was confirmed by immunoblotting. We detected the 6-fold (p < 0.001), 4.5-fold (p < 0.001), and 7.5-fold (p < 0.001) enhancement of cleaved caspase-3, cleaved caspase-9, and Bax (B-cell lymphoma 2-associated X) protein level expression, respectively (Figure 5C), and we also recorded a reduction in Bcl2 (B-cell lymphoma 2) protein expression level in a dose-dependent manner (Figure 5C and 5D). Furthermore, to validate our results, we detected the cleaved caspase-3 levels in the cells by using an immunofluorescence assay and observed a dose-dependent increase (Figure 5E).

Figure 5

Nitrogen-/phosphorous-doped carbon dots (NPCDs; 0-30 μL/mL)-induced apoptotic morphology in B16F10 melanoma cancer cells. Apoptotic morphology was evaluated by A) live-dead cells and B) Hoechst-PI staining. (C) Western blot analysis of various apoptotic markers (caspase-3, Bcl2, and Bax) and Image J analysis. (D) Immunocytochemistry of prominent apoptosis marker cleaved caspase-3 after treatment with NPCDs evaluated via confocal microscopy. The data are represented as the mean ± standard deviation (SD) of three independent experiments: ***P < 0.001, **P < 0.01, * P < 0.05 vs. the control. Fluorescence images were captured at 20× magnification (scale bar = 0.1 mm).

Oxidative burst and mitochondrial membrane potential

Our results obtained via n class="Chemical">H2DCFDA staiclass="Chemical">niclass="Chemical">ng of class="Chemical">n class="CellLine">B16F10 cells indicate that the oxidative stress was enhanced, as manifested by the dose-dependent increase in green fluorescence after treatment with NPCDs (Figure 6A), although the oxidative stress yielded a decrease in the mitochondrial membrane potential (Δψm). Similarly, the Rhodamine-123 fluorescence decreased in a dose-dependent manner in the B16F10 cells (Figure 6B).

Figure 6

Nitrogen-/phosphorous-doped carbon dots (NPCDs; 0-30 μL/mL)-induced cytosolic and mitochondrial ROS production in B16F10 melanoma cancer cells. (A) cytosolic ROS production determined by H2DCFDA counterstaining with DAPI and (B) Rhodamine 123 staining detected by fluorescence microscopy. Fluorescence intensities of (C) cytosolic and (D) mitochondrial ROS showing results corresponding to (i) the control and (ii) 5, (iii) 15, and (iv) and 30 μL/mL of NPCDs. ImageJ software was used to plot their respective intensities. Fluorescence images were captured at 20× magnification (scale bar = 0.1 mm). The data are represented as the mean ± standard deviation (SD) of three independent experiments: ***P < 0.001, **P < 0.01, * P < 0.05 vs. the control.

NPCDs provoke cell cycle arrest in B16F10 cells

The role of n class="Chemical">NPCDs iclass="Chemical">n cell cycle class="Chemical">n class="Disease">arrest was evaluated by examining the expression levels of CDK-2, -4, and -6 and p21. Figure 7A reveals a significant (p < 0.05) dose-dependent reduction in the expression levels of CDK-2, -4, and -6 and a marked increase in p21 expression in the B16F10 cells treated with NPCDs (Figure 7B). All of the results collectively suggest that the NPCDs induce cell senescence and cell cycle arrest in B16F10 cells. Furthermore, the obtained results of immunoblotting were further validated by using an immunofluorescence assay. We detected a dose-dependent reduction in fluorescence of CDK-4 counterstained with DAPI (Figure 7C).

Figure 7

Effect of nitrogen-/phosphorous-doped carbon dots on cell cycle arrest markers. (A) Western blot analysis of various cell cycle arrest markers (CDK-2, -4, and -6 and p21) and (B) ImageJ analysis of B16F10 melanoma cancer cells after treatment with nitrogen-/phosphorous-doped carbon dots (NPCDs; 0-30 μL/mL). (C) Immunocytochemistry of cell cycle arrest marker CDK-4 after treatment with NPCDs evaluated via confocal microscopy. The data are represented as the mean ± standard deviation (SD) of three independent experiments: ***P < 0.001, **P < 0.01, * P < 0.05 vs. the control.

NPCDs induce autophagy in B16F10 cells

The role of autophagy in cell survival and death has been reported in several studies. Therefore, to further investigate the role of the n class="Chemical">NPCDs iclass="Chemical">n this process, we evaluated the level of class="Chemical">n class="Chemical">crucial autophagic markers such as LC3 (microtubule-associated protein 1A/1B-light chain 3), which revealed the elevated dose-dependent expression level of the LC3-phosphatidylethanolamine (LC3-II) marker (Figure 8A). The results revealed that, in addition to LC3-II and ATG-5 (autophagy-related-5) also being upregulated in a dose-dependent manner (Figure 8A), NPCD treatment also resulted in sequestosome-1 (p62/SQSTM1) degradation. These results indicate that NPCDs lead to autophagic cell death of B16F10 melanoma cells (Figure 8B). Furthermore, the obtained immunoblotting data was confirmed by additional experiments such as immunofluorescence measurements. We detected the dose-dependent increase in LC3-II fluorescence counterstained with DAPI, which is a hallmark of autophagy, after treatment with the NPCDs (Figure 8C). Moreover, we used AO and LysoTracker Red to detect the autophagy levels (Figure 8D and 8E) and validated the results with 3-MA (methyladenine) as an ATG-5 inhibitor (Figure 8F and 8G). The obtained results indicate that the NPCDs induce autophagy in a dose-dependent manner, as validated by the autophagy inhibitor.

Figure 8

Effect of nitrogen-/phosphorous-doped carbon dots on autophagy markers. (A) Western blot analysis of various autophagy markers (LC3, ATG-5, and p62) and (B) ImageJ analysis of B16F10 melanoma cancer cells after treatment with nitrogen-/phosphorous-doped carbon dots (NPCDs; 0-30 μL/mL). (C) Immunocytochemistry of prominent autophagy marker LC3 after treatment with NPCDs evaluated via confocal microscopy. (D) Autophagic vacuole formation after treatment with NPCDs detected via confocal microscopy. (E) ImageJ analysis of autophagic vacuole formation. (F and G) Validation of autophagic vacuole formation evaluated in the presence of 3-methyladenine ((3-MA) preincubated with 2.5 mM for 1 h and then incubated with NPCDs for 24h). The data are represented as the mean ± standard deviation (SD) of three independent experiments: ***P < 0.001, **P < 0.01, * P < 0.05 vs. the control; ###P < 0.001, ##P < 0.01, # P < 0.05 vs. the control.

Autophagy attenuation

To determine the role of autophagy after induction with n class="Chemical">NPCDs, we did geclass="Chemical">ne sileclass="Chemical">nciclass="Chemical">ng of class="Chemical">n class="Gene">atg5 by using siRNA and evaluated normalized gene expression (atg5, lc-3, p62, and caspase-3) by real-time PCR analysis. Knockdown was executed to down-regulate atg5, an important molecule for the initial formation of autophagosome and results were determined by real-time PCR (Figure S4). Our obtained data denoted that atg5 was significantly down-regulated by siRNA at 48 h detected by RT-PCR. This down-regulation of atg5 also suppressed autophagy which was evident by reduced lc-3 gene levels in B16F10 cells. When atg5 silenced cells were treated in the presence of NPCDs, lc-3 and p62 levels were also increased compared to the atg5 silenced cells. Moreover, we also observed no change of caspase3 in atg5 silenced group compared to control, however, similar levels of caspase3 gene were observed under NPCDs as well as combination of atg5 siRNA and NPCDs-treated group compared to control group, which indicated that cell death occurred by apoptotic pathway in B16F10 cells even after knockdown of atg5.

Application of NPCDs as nanocarriers in cellular labeling/imaging

We used the highly fluorescent n class="Chemical">NPCDs for cellular imagiclass="Chemical">ng. As depicted iclass="Chemical">n Figure 9, treatmeclass="Chemical">nt of the class="Chemical">n class="CellLine">B16F10 melanoma cells with NPCDs at different concentrations (1, 5, and 10 µL/mL) was found to be very effective in cell bioimaging, as confirmed by the cells emitting green, red, and blue fluorescence at ex: 500-570 nm, ex: 610-750 nm, and ex: 450-500 nm, respectively, under an inverted microscope (Figure 9); treatment with the fluorescent NPCDs made the cells easily visible. At 1 and 5 µL/mL NPCD concentrations, the green, red, and blue fluorescence were found to be slightly enhanced, but it was found to be the highest at 10 µL/mL. We detected the fluorescence from 450 to 750 nm and observed the dose-dependent enhancement of fluorescence.

Figure 9

Application of nitrogen-/phosphorous-doped carbon dots (NPCDs) in B16F10 melanoma cell bioimaging. Treatment of the B16F10 melanoma cancer cells gave rise to dose-dependent green, red, and blue fluorescence after 30 min of incubation in the dark. Cells emitting blue, green, and red fluorescence at, ex: 450-500 nm, ex: 500-570 nm and ex: 610-750 nm.

Discussion

n class="Disease">Skin cancer is the most commoclass="Chemical">nly diagclass="Chemical">nosed class="Chemical">n class="Disease">cancer in the US, and most cases are preventable 43, 44. Skin cancer greatly affects the quality of life, and it can be disfiguring or even deadly 43. Medical treatment for skin cancer creates substantial healthcare costs for individuals, families, and the nation. The number of Americans who have had skin cancer at some point in the last three decades is estimated to be higher than the number for all other cancers combined 30-45, and skin cancer incidence rates have continued to increase in recent years 43-46.

Each year in the US, nearly 5 million n class="Species">people are treated for all class="Chemical">n class="Disease">skin cancers combined, with an annual cost estimated at $8.1 billion 47. Among all skin cancers, melanoma is responsible for the most deaths, with nearly 9,000 each year 48. It is also one of the most common types of cancer among US adolescents and young adults 49. Around $3.3 billion of annual skin cancer treatment costs are attributable to melanoma cancer 47.

A recent addition to the n class="Chemical">carbon class="Chemical">naclass="Chemical">nomaterial family, quaclass="Chemical">ntum class="Chemical">n class="Chemical">CDs, have attracted great interest because of their outstanding water solubility, high sensitivity and selectivity for target analytes, strong disease detection ability, low toxicity, favorable biocompatibility, and excellent photostability 50. Various kinds of quantum CDs have been used as biomedical and biocontrol agents 51. Quantum dots (QDs) are generally semiconductor nanocrystals of physical dimensions smaller than the exciton Bohr radius 52. Because of the quantum-confinement effect, the electronic and optical properties of QDs exhibit characteristic dependencies on nanoparticle size and composition, especially the infamous display of beautiful fluorescence colors for QDs of varying semiconductor nanoparticle sizes. The high optical performance of QDs has captured the imagination of researchers in biomedical fields, with the exploration of a wide variety of potential bio-applications already producing exciting results. However, there have not yet been any reports on the anticancer activity of doped QDs. Therefore, in this study, the NPCDs were synthesized using PEG as a C source, imidazole as an N source, and phosphoric acid as a P source. They were then characterized by FTIR, XPS, UV-Vis, HRTEM, and fluorescence analyses.

In our study, we employed the n class="Chemical">NPCDs to evaluate their aclass="Chemical">nticlass="Chemical">n class="Disease">cancer potential on B16F10 melanoma cells. Initially, we explored their anticancer as well as apoptotic activity and found that the NPCDs exert apoptotic activity on B16F10 cells. Moreover, apoptosis is a highly energy-dependent process governed by activation and or inactivation of myriad arrays of proteins 3. It is crucial to understand the complex process of apoptosis, which is comprised of DNA degradation, chromatin condensation, and shrinkage of cell, as well as blebbing 3. We found that the NPCDs caused apoptotic cell and apoptotic nuclei formation, which is in accordance with a previous report on the apoptotic potential of carboxylic acid-coated CDs against human keratinocytes 53.

Under stressful conditions, cells undergo much cellular orchestration to organize many cellular signaling cascades, including activation of oxidative stress 4, 37 which is the main protagonist of most apoptotic cell death. Activated n class="Chemical">ROS class="Chemical">not oclass="Chemical">nly fuclass="Chemical">nctioclass="Chemical">n to activate further cellular eveclass="Chemical">nts but also class="Chemical">n class="Chemical">create an imbalance among various organelles such as mitochondria 37. The disturbed homeostasis of the mitochondria causes dysregulation of Bax and Bcl2 protein levels 54. Moreover, oxidative imbalance causes weakening of the mitochondrial membrane, leading to the release of cytochrome C from the mitochondria 50, this phenomenon is very well known for insinuating the activation of proteases such caspases, thereby leading to apoptotic cell death 55. In this study, we found that the NPCDs caused B16F10 cells to undergo oxidative stress and then mitochondrial-mediated caspase-dependent apoptosis. Similarly, Nguyen et al. 56 reported that exposure to cadmium telluride QDs led to oxidative stress and apoptosis in HepG2 cells.

The cell cycle is composed of four phases by activation or inactivation of cyclin-dependent kinases depending upon the status of the cell and the surrounding environment 51. The initial phase involves n class="Gene">cyclin D1, class="Chemical">n class="Gene">CDK-4, and CDK-6 that help to build the G1 phase, which is known to be crucial for the formation of the cell cycle. The cell then enters the synthesis phase where cyclin E and CDK-2 are needed for DNA synthesis. In the subsequent G2 to M transition phase, the Cdc2 (cell division control protein kinase 2)-cyclin B complex is required. Many commercially available drugs hamper the cell cycle, leading to cell death. Similarly, the NPCDs were found to arrest the cell cycle by downregulating the CDK complex and upregulating p21 protein levels. Relatedly, graphene QDs were also reported by Sui et al. 51 to be responsible for causing cell cycle arrest and cell death.

Along with the cell cycle and apoptosis, autophagy is also responsible for causing cell death or survival depending upon the cell fate 57. Autophagy is a lysosomal catabolic process utilized by cells to eradicate damaged and non-functional proteins or metabolites 6. In the past few years, research has revealed that autophagy has a greater variety of pathophysiological functions than was previously known 6. Mitochondrial membrane weakening also triggers the autophagic process, subsequently leading to autophagic cell death 58. Similarly, we explored the role of n class="Chemical">NPCDs iclass="Chemical">n autophagic cell death aclass="Chemical">nd the overall mechaclass="Chemical">nism of their aclass="Chemical">nticlass="Chemical">n class="Disease">cancer effects is presented in Figure 10.

Figure 10

Anticancer mechanism of nitrogen-/phosphorous-doped carbon dots (NPCDs) against B16F10 melanoma cells.

Based on the non-toxic and remarkable fluorescence performance of the n class="Chemical">NPCDs iclass="Chemical">n our study, iclass="Chemical">n vitro cellular imagiclass="Chemical">ng was coclass="Chemical">nducted oclass="Chemical">n class="Chemical">n class="CellLine">B16F10 melanoma cells. The distinctive advantages of CDs are their multicolor emission profile, small size, low cytotoxicity, prominent biocompatibility, and excellent photostability, making them ideal candidates for fluorescence imaging 59. As expected, strong green and red fluorescence were obtained at the wavelengths of green (500-570 nm), red (610-750 nm), and blue (450-500 nm) from the NPCD-labeled B16F10 melanoma cancer cells. This indicates that the NPCDs were effectively taken up by the B16F10 melanoma cancer cells and could be used as fluorescent probes for cellular imaging that addresses many issues in different fields of research, such as alternatives for chemical substances commonly used to stain (dye) drug delivery and cell labeling. Similarly, Vasimalai et al. 60 also explored the use of CDs as high-performance, non-toxic fluorescence agents for optical in vitro bioimaging of cancer cells such as human glioblastoma cells (the LN-229 cancer cell line). In general, staining cancer cells is important because without it, most cancer cells are extremely difficult to see. Staining allows them to be seen so that observations as to their morphology (i.e. individual cell shape) and arrangement (i.e. how the cells remain physically attached to one another after cell division) can be made. Based on these results, we can assume that the NPCDs could be effectively used for cancer cell imaging 12, 61.

Conclusions

To summarize, we herein report the synthesis and characterization of multifunctional and biocompatible n class="Chemical">NPCDs by meaclass="Chemical">ns of a greeclass="Chemical">n thermal treatmeclass="Chemical">nt method. We democlass="Chemical">nstrated the sigclass="Chemical">nificaclass="Chemical">nt aclass="Chemical">nticlass="Chemical">n class="Disease">cancer potential of NPCDs on a melanoma cell line and explored the pathways that allow the NPCD mediation of cell death. More precisely, we found that the NPCDs regulate the cell death pathway by inducing apoptosis, cell cycle arrest, and autophagy. Application of NPCDs for cellular bioimaging of B16F10 melanoma cells further validated their usefulness in the diagnosis of cancer-associated diseases. Therefore, our green-synthesized NPCDs could be useful in the detection and treatment of skin cancer, although in vivo studies are needed to further validate their practical application.

Supplementary figures and tables.

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