Shaohui Yang1, Yue Feng2, Yue Zhao2, Jingping Bai2, Jiehua Wang2. 1. School of Environmental Science and Engineering, Tianjin University, Nankai Area, Weijin Rd. 92, Tianjin, 300072, China. shaohuiyang77@tju.edu.cn. 2. School of Environmental Science and Engineering, Tianjin University, Nankai Area, Weijin Rd. 92, Tianjin, 300072, China.
Abstract
OBJECTIVE: To enhance Pi absorption and utilization efficiency of soybean, a member of PHT1 gene family was isolated and characterized from E. salsugineum, which was a homologous gene of AtPHT1;4 and consequently designated as EsPHT1;4. RESULTS: Quantitative real-time PCR (qRT-PCR) analysis showed that the transcript level of EsPHT1;4 significantly increased both in roots and leaves of E. salsugineum under Pi deficient conditions. Furthermore, EsPHT1;4 was transferred to soybean cultivar "YD22" using an Agrobacterium-mediated cotyledonary-node transformation method. Overexpression of EsPHT1;4 in soybean not only promoted the increase of plant biomass and yield of transgenic plants upon low P stress, but also increased the accumulation and transportation of Pi from roots to leaves in the transgenic soybean lines. CONCLUSION: EsPHT1;4 was critical for controlling the accumulation and translocation of Pi in plants, and can be subsequently used as an effective foreign gene for the improvement of P use efficiency of crops by genetic manipulation.
OBJECTIVE: To enhance Pi absorption and utilization efficiency of soybean, a member of PHT1 gene family was isolated and characterized from E. salsugineum, which was a homologous gene of AtPHT1;4 and consequently designated as EsPHT1;4. RESULTS: Quantitative real-time PCR (qRT-PCR) analysis showed that the transcript level of EsPHT1;4 significantly increased both in roots and leaves of E. salsugineum under Pi deficient conditions. Furthermore, EsPHT1;4 was transferred to soybean cultivar "YD22" using an Agrobacterium-mediated cotyledonary-node transformation method. Overexpression of EsPHT1;4 in soybean not only promoted the increase of plant biomass and yield of transgenic plants upon low P stress, but also increased the accumulation and transportation of Pi from roots to leaves in the transgenic soybean lines. CONCLUSION: EsPHT1;4 was critical for controlling the accumulation and translocation of Pi in plants, and can be subsequently used as an effective foreign gene for the improvement of P use efficiency of crops by genetic manipulation.
Authors: Günsu Inan; Quan Zhang; Pinghua Li; Zenglan Wang; Ziyi Cao; Hui Zhang; Changqing Zhang; Tanya M Quist; S Mark Goodwin; Jianhua Zhu; Huazhong Shi; Barbara Damsz; Tarif Charbaji; Qingqiu Gong; Shisong Ma; Mark Fredricksen; David W Galbraith; Matthew A Jenks; David Rhodes; Paul M Hasegawa; Hans J Bohnert; Robert J Joly; Ray A Bressan; Jian-Kang Zhu Journal: Plant Physiol Date: 2004-07-09 Impact factor: 8.340