Mark D Skopin1, Arezou Bayat1, Lalitha Kurada1, Mithilesh Siddu1, Sweta Joshi1, Christina M Zelano2, Mohamad Z Koubeissi3. 1. Department of Neurology, George Washington University, Washington, DC, 20037, USA. 2. Department of Neurology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA. 3. Department of Neurology, George Washington University, Washington, DC, 20037, USA. Electronic address: mkoubeissi@mfa.gwu.edu.
Abstract
OBJECTIVE: Tissue remodeling has been described in brain circuits that are involved in the generation and propagation of epileptic seizures. Human and animal studies suggest that the anterior piriform cortex (aPC) is crucial for seizure expression in focal epilepsies. Here, we investigate the effect of kainic-acid (KA)-induced seizures on the effective connectivity of the aPC with bilateral hippocampal CA3 regions using cerebro-cerebral evoked potentials (CCEPs). METHODS: Adult male Sprague-Dawley rats were implanted with a tripolar electrode in the left aPC for stimulation and recording, and with unipolar recording electrodes in bilateral CA3 regions. Single pulse stimulations were given to the aPC and CCEPs were averaged before KA injections and after the emergence of spontaneous recurrent seizures (SRS). Similar recordings at equivalent time intervals were obtained from animals that received saline injections instead of KA (controls). RESULTS: In the experimental group, the percentage change of increased amplitude of the contralateral (but not ipsilateral) CA3 CCEPs between pre-KA injection and after the emergence of SRS was significantly greater than in controls. No significant single-pulse-induced spectral change responses were observed in either epileptic or control rats when comparing pre- and post-stimulus time intervals. Also, we found no correlation between seizure frequency and the extent of amplitude changes in the CCEPs. CONCLUSIONS: In the KA model, epileptogenesis results in plastic changes that manifest as an amplification of evoked potential amplitudes recorded in the contralateral hippocampus in response to single-pulse stimulation of the aPC. These results suggest epileptogenesis-induced facilitation of interhemispheric connectivity between the aPC and the hippocampus. Since the amplitude increase of the contralateral CCEP is a possible in vivo biomarker of epilepsy, any intervention (e.g. neuromodulatory) that can reverse this phenomenon may hold a potential antiepileptic efficacy.
OBJECTIVE: Tissue remodeling has been described in brain circuits that are involved in the generation and propagation of epileptic seizures. Human and animal studies suggest that the anterior piriform cortex (aPC) is crucial for seizure expression in focal epilepsies. Here, we investigate the effect of kainic-acid (KA)-induced seizures on the effective connectivity of the aPC with bilateral hippocampal CA3 regions using cerebro-cerebral evoked potentials (CCEPs). METHODS: Adult male Sprague-Dawley rats were implanted with a tripolar electrode in the left aPC for stimulation and recording, and with unipolar recording electrodes in bilateral CA3 regions. Single pulse stimulations were given to the aPC and CCEPs were averaged before KA injections and after the emergence of spontaneous recurrent seizures (SRS). Similar recordings at equivalent time intervals were obtained from animals that received saline injections instead of KA (controls). RESULTS: In the experimental group, the percentage change of increased amplitude of the contralateral (but not ipsilateral) CA3 CCEPs between pre-KA injection and after the emergence of SRS was significantly greater than in controls. No significant single-pulse-induced spectral change responses were observed in either epileptic or control rats when comparing pre- and post-stimulus time intervals. Also, we found no correlation between seizure frequency and the extent of amplitude changes in the CCEPs. CONCLUSIONS: In the KA model, epileptogenesis results in plastic changes that manifest as an amplification of evoked potential amplitudes recorded in the contralateral hippocampus in response to single-pulse stimulation of the aPC. These results suggest epileptogenesis-induced facilitation of interhemispheric connectivity between the aPC and the hippocampus. Since the amplitude increase of the contralateral CCEP is a possible in vivo biomarker of epilepsy, any intervention (e.g. neuromodulatory) that can reverse this phenomenon may hold a potential antiepileptic efficacy.
Authors: H Laufs; M P Richardson; A Salek-Haddadi; C Vollmar; J S Duncan; K Gale; L Lemieux; W Löscher; M J Koepp Journal: Neurology Date: 2011-08-17 Impact factor: 9.910
Authors: Marta S Vismer; Patrick A Forcelli; Mark D Skopin; Karen Gale; Mohamad Z Koubeissi Journal: Front Neural Circuits Date: 2015-05-29 Impact factor: 3.492
Authors: Guangyu Zhou; Jonas K Olofsson; Mohamad Z Koubeissi; Georgios Menelaou; Joshua Rosenow; Stephan U Schuele; Pengfei Xu; Joel L Voss; Gregory Lane; Christina Zelano Journal: Prog Neurobiol Date: 2021-02-25 Impact factor: 10.885