| Literature DB >> 32681516 |
Anna Postepska-Igielska1, Alena Blank-Giwojna1, Ingrid Grummt2.
Abstract
RNA can bind within the major groove of purine-rich DNA via Hoogsteen base pairing and form a triple helical RNA-DNA structure that anchors the RNA to specific DNA sequences, thereby targeting RNA-associated regulatory proteins to distinct genomic sites. Here we present methods to analyze the potential of a given RNA to form triplexes in vitro and to validate these structures in vivo.Keywords: Biotinylated RNA; Electrophoretic mobility shift assay; Noncoding RNA; Psoralen; Pull-down; RNA–DNA triplexes; Triplex capture
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Year: 2020 PMID： 32681516 DOI： 10.1007/978-1-0716-0680-3_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745