| Literature DB >> 32661401 |
Ahmed E Hozain1,2, John D O'Neill1, Matthew Bacchetta3,4,5,6,7, Gordana Vunjak-Novakovic8,9, Meghan R Pinezich1, Yuliya Tipograf2, Rachel Donocoff10, Katherine M Cunningham1, Andrew Tumen11, Kenmond Fung12, Rei Ukita11, Michael T Simpson2, Jonathan A Reimer1,2, Edward C Ruiz1, Dawn Queen13, John W Stokes11, Nancy L Cardwell11, Jennifer Talackine11, Jinho Kim1,14, Hans-Willem Snoeck15,16,17, Ya-Wen Chen15,18,19, Alexander Romanov10, Charles C Marboe20, Adam D Griesemer2,21, Brandon A Guenthart1,22.
Abstract
Patients awaiting lung transplantation face high wait-list mortality, as injury precludes the use of most donor lungs. Although ex vivo lung perfusion (EVLP) is able to recover marginal quality donor lungs, extension of normothermic support beyond 6 h has been challenging. Here we demonstrate that acutely injured human lungs declined for transplantation, including a lung that failed to recover on EVLP, can be recovered by cross-circulation of whole blood between explanted human lungs and a Yorkshire swine. This xenogeneic platform provided explanted human lungs a supportive, physiologic milieu and systemic regulation that resulted in functional and histological recovery after 24 h of normothermic support. Our findings suggest that cross-circulation can serve as a complementary approach to clinical EVLP to recover injured donor lungs that could not otherwise be utilized for transplantation, as well as a translational research platform for immunomodulation and advanced organ bioengineering.Entities:
Mesh：
Year: 2020 PMID： 32661401 DOI： 10.1038/s41591-020-0971-8
Source DB: PubMed Journal: Nat Med ISSN： 1078-8956 Impact factor: 87.241