| Literature DB >> 32661383 |
Qian Li1, Jiemin Zhao1, Longfei Liu1, Sagun Jonchhe2, Felix J Rizzuto3, Shankar Mandal2, Huawei He4, Sansen Wei1, Hanadi F Sleiman5, Hanbin Mao6,7, Chengde Mao8,9.
Abstract
The diversity of DNA duplex structures is limited by a binary pair of hydrogen-bonded motifs. Here we show that poly(thymine) self-associates into antiparallel, right-handed duplexes in the presence of melamine, a small molecule that presents a triplicate set of the hydrogen-bonding face of adenine. X-ray crystallography shows that in the complex two poly(thymine) strands wrap around a helical column of melamine, which hydrogen bonds to thymine residues on two of its three faces. The mechanical strength of the thymine-melamine-thymine triplet surpasses that of adenine-thymine base pairs, which enables a sensitive detection of melamine at 3 pM. The poly(thymine)-melamine duplex is orthogonal to native DNA base pairing and can undergo strand displacement without the need for overhangs. Its incorporation into two-dimensional grids and hybrid DNA-small-molecule polymers highlights the poly(thymine)-melamine duplex as an additional tool for DNA nanotechnology.Entities:
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Year: 2020 PMID： 32661383 PMCID： PMC7732259 DOI： 10.1038/s41563-020-0728-2
Source DB: PubMed Journal: Nat Mater ISSN： 1476-1122 Impact factor: 47.656