In vitro growth of Colletotrichum gloeosporioides is affected by butyl acetate, a compound produced during the co-culture of Trichoderma sp. and Bacillus subtilis.

The co-culture of plant beneficial microbes to stimulate the production of antimicrobial metabolites is gaining ground. Here, the inactivated Colletotrichum gloeosporioides mycelium was used to induce the biosynthesis of antifungal compounds in the co-culture systems of Trichoderma sp. and Bacillus subtilis. The hexanic extracts obtained from the co-culture systems were tested against C. gloeosporioides. Those that inhibited the phytopathogen growth were further fractionated by column and thin-layer chromatography and analyzed by gas chromatography coupled to mass spectrometry (GC-MS). Ethyl butanoate, butyl acetate, acetic acid, 2-butoxyethanol, 3,5-di-tert-butyl-4-hydroxybenzaldehyde, 3,5-di-tert-butyl-4-hydroxybenzyl alcohol, hexadecanoic acid, and octadecanoic acid were identified. Butyl acetate was the most abundant compound, and its application affected the morphology and mycelial development of C. gloeosporioides, thereby inhibiting the radial growth, reducing spore formation, and inducing soft colonies. We conclude that co-culturing Trichoderma sp. and B. subtilis promotes the production of novel diffusible organic compounds with an antifungal effect on C. gloeosporioides. © King Abdulaziz City for Science and Technology 2020.