Jianhua Dai1, Danfeng Liu1, Longjiang Chen1, LiXia Sun2. 1. Department of Hepatobiliary Surgery, The Second People's Hospital of Wuhu, No. 259, Jiuhua Middle Road, Wuhu, 241001, Anhui, China. 2. Department of Hepatobiliary Surgery, The Second People's Hospital of Wuhu, No. 259, Jiuhua Middle Road, Wuhu, 241001, Anhui, China. huakuiyan225@163.com.
Abstract
PURPOSE: To investigate the effect of Ag-1031 on apoptosis of gastric cancer AGS cells and the PI3K/AKT/mTOR signaling pathway. METHODS: Gastric cancer cells were treated with different doses of Ag-1031. Cell viability was determined with MTT assay. Apoptosis was analyzed with Hoechst 33342 staining and Annexin V-FITC/PI double staining. The protein expression levels of p-PI3K, p-AKT, p-mTOR, PI3K, AKT and mTOR were assayed with western blotting method. RESULTS: Different doses of Ag-1031 significantly inhibited the proliferation of AGS cells. Moreover, Ag-1031 induced apoptosis,in gastric cancer cells. In addition, Ag-1031 significantly and dose-dependently inhibited the protein expressions of p-PI3K, p-AKT and p-mTOR. However, it had no effect on the protein expressions of PI3K, AKT and mTOR. CONCLUSION: In this study, it was found for the first time that Ag-1031 exerts an anti-tumor effect by induction apoptosis in gastric cancer AGS cells, through a mechanism related to inhibition of the activation of the PI3K/AKT/mTOR signaling pathway.
PURPOSE: To investigate the effect of Ag-1031 on apoptosis of gastric cancer AGS cells and the PI3K/AKT/mTOR signaling pathway. METHODS:Gastric cancer cells were treated with different doses of Ag-1031. Cell viability was determined with MTT assay. Apoptosis was analyzed with Hoechst 33342 staining and Annexin V-FITC/PI double staining. The protein expression levels of p-PI3K, p-AKT, p-mTOR, PI3K, AKT and mTOR were assayed with western blotting method. RESULTS: Different doses of Ag-1031 significantly inhibited the proliferation of AGS cells. Moreover, Ag-1031 induced apoptosis,in gastric cancer cells. In addition, Ag-1031 significantly and dose-dependently inhibited the protein expressions of p-PI3K, p-AKT and p-mTOR. However, it had no effect on the protein expressions of PI3K, AKT and mTOR. CONCLUSION: In this study, it was found for the first time that Ag-1031 exerts an anti-tumor effect by induction apoptosis in gastric cancer AGS cells, through a mechanism related to inhibition of the activation of the PI3K/AKT/mTOR signaling pathway.