BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia is a newly recognized disease, and its diagnosis is primarily confirmed by routine reverse transcriptase -polymerase chain reaction (RT-PCR) detection of SARS-CoV-2. METHODS: However, we report a confirmed case of SARS-CoV-2 pneumonia with a negative routine RT-PCR. RESULTS: This case was finally diagnosed by nanopore sequencing combined with antibody of SARS-CoV-2. Simultaneously, the ORF and NP gene variations of SARS-CoV-2 were found. CONCLUSIONS: This case highlighted that false-negative results could be present in routine RT-PCR diagnosis, especially with virus variation. Currently, nanopore pathogen sequencing and antibody detection have been found to be effective in clinical diagnosis.
BACKGROUND:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia is a newly recognized disease, and its diagnosis is primarily confirmed by routine reverse transcriptase -polymerase chain reaction (RT-PCR) detection of SARS-CoV-2. METHODS: However, we report a confirmed case of SARS-CoV-2 pneumonia with a negative routine RT-PCR. RESULTS: This case was finally diagnosed by nanopore sequencing combined with antibody of SARS-CoV-2. Simultaneously, the ORF and NP gene variations of SARS-CoV-2 were found. CONCLUSIONS: This case highlighted that false-negative results could be present in routine RT-PCR diagnosis, especially with virus variation. Currently, nanopore pathogen sequencing and antibody detection have been found to be effective in clinical diagnosis.
Authors: Stephanie Popping; Richard Molenkamp; Joachim D Weigel; Pim G N J Mutsaers; Jolanda C Voermans; Sander van Boheemen; Marc C Shamier; Reina S Sikkema; David F Nieuwenhuijse; Bas B Oude Munnink; Marion P G Koopmans; Jeroen J A van Kampen Journal: J Virol Methods Date: 2021-12-01 Impact factor: 2.014
Authors: Asmaa M Altamimi; Dalia A Obeid; Taghreed A Alaifan; Moroje T Taha; Marwa T Alhothali; Fahad A Alzahrani; Ahmad M Albarrag Journal: J Med Virol Date: 2021-03-17 Impact factor: 20.693
Authors: Ki Ho Hong; Ji Won In; Jaehyeon Lee; So Yeon Kim; Kyoung Ah Lee; Seunghyun Kim; Yeoungim An; Donggeun Lee; Heungsup Sung; Jae-Seok Kim; Hyukmin Lee Journal: Ann Lab Med Date: 2022-01-01 Impact factor: 3.464