Adriana Yuriko Koga1, Julio César Felix2, Rodrigo Gomes Marques Silvestre3, Leandro Cavalcante Lipinski4, Bruna Carletto5, Fernanda Alexia Kawahara6, Airton Vicente Pereira7. 1. Fellow PhD degree, Postgraduate Program in Pharmaceutical Sciences, Universidade Estadual de Ponta Grossa (UEPG), Brazil. Technical procedures, analysis and interpretation of data, manuscript writing. 2. Professor, Director in Technology Institute of Paraná, Curitiba-PR, Brazil. Scientific, conception and design of the study. 3. Master, Industrial Director in Technology Institute of Paraná, Curitiba-PR, Brazil. Scientific, conception and design of the study. 4. PhD, Associate Professor, Department of Medicine, UEPG, Ponta Grossa-PR, Brazil. Design of the study, technical procedures, statistical analysis, interpretation of data, critical revision, final approval. 5. Fellow PhD degree, Postgraduate Program in Pharmaceutical Sciences, UEPG, Ponta Grossa-PR, Brazil. Technical procedures, manuscript writing. 6. Master, Postgraduate Program in Biomedical Science, UEPG, Ponta Grossa- PR, Brazil. Design of the study, technical procedures. 7. PhD, Associate Professor, Department of Pharmaceutical Science, UEPG, Ponta Grossa-PR, Brazil. Design of the study, technical procedures, interpretation of data, manuscript writing, critical revision, final approval.
Abstract
PURPOSE: To develop a new wound dressing composed of alginate and Aloe vera gel and cross-linked with zinc ions. METHODS: The aloe-alginate film was characterized using scanning electron microscopy (SEM), swelling profile, mechanical properties, polysaccharide content and X-ray diffraction (XRD). Thirty Wistar rats were divided in two groups a) treated with aloe-alginate film and b) control (treated with sterile gauze). Wound contraction measurements and hystological analysis were performed on 7th, 14th and 21st days after wound surgery. RESULTS: The aloe-alginate film presented adequated mechanical resistance and malleability for application as wound dressing. There was no statistical difference in wound contraction between two groups. Histological assay demonstrated that aloe-alginate film presented anti-inflammatory activity, stimulated angiogenesis on proliferative phase and a more significant increased in collagen type I fibers and decreased type III fibers which promoted a mature scar formation when compared to control. CONCLUSIONS: The aloe-alginate film showed adequate physicochemical characteristics for wound dressing applications. The in vivo assay demonstrated that aloe-alginate film enhanced the healing process of incisional skin wounds.
PURPOSE: To develop a new wound dressing composed of alginate and Aloe vera gel and cross-linked with zinc ions. METHODS: The aloe-alginate film was characterized using scanning electron microscopy (SEM), swelling profile, mechanical properties, polysaccharide content and X-ray diffraction (XRD). Thirty Wistar rats were divided in two groups a) treated with aloe-alginate film and b) control (treated with sterile gauze). Wound contraction measurements and hystological analysis were performed on 7th, 14th and 21st days after wound surgery. RESULTS: The aloe-alginate film presented adequated mechanical resistance and malleability for application as wound dressing. There was no statistical difference in wound contraction between two groups. Histological assay demonstrated that aloe-alginate film presented anti-inflammatory activity, stimulated angiogenesis on proliferative phase and a more significant increased in collagen type I fibers and decreased type III fibers which promoted a mature scar formation when compared to control. CONCLUSIONS: The aloe-alginate film showed adequate physicochemical characteristics for wound dressing applications. The in vivo assay demonstrated that aloe-alginate film enhanced the healing process of incisional skin wounds.