| Literature DB >> 3263467 |
D L Rosenstreich1, J H Tu, P R Kinkade, I Maurer-Fogy, J Kahn, R W Barton, P R Farina.
Abstract
We have previously reported that the urine of febrile humans contained large quantities of an inhibitor of IL-1-induced murine thymocyte proliferation that was a glycoprotein between 30 and 40 kD in size. In the present study this factor has been purified to homogeneity using a sequence of eight purification steps (ammonium sulfate precipitation, ion exchange chromatography, molecular sieve chromatography, hydrophobic affinity chromatography, hydroxylapatite chromatography, fast protein liquid chromatography, and two HPLC steps). SDS-PAGE analysis indicates that the purified material is a 38-kD molecule. Evidence based on a partial amino acid sequence analysis as well as enzyme studies indicates that this inhibitor is a type of human DNase I.Entities:
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Year: 1988 PMID： 3263467 PMCID： PMC2189114 DOI： 10.1084/jem.168.5.1767
Source DB: PubMed Journal: J Exp Med ISSN： 0022-1007 Impact factor: 14.307