Literature DB >> 32632854

Isolation and Culture of Leptospira from Clinical and Environmental Samples.

Cyrille Goarant1, Dominique Girault2, Roman Thibeaux2, Marie-Estelle Soupé-Gilbert2.   

Abstract

Leptospires, the etiological agents of leptospirosis, are fastidious slow-growing organisms. Here we describe the isolation and routine maintenance of leptospires from clinical (blood, urine, or tissue) and environmental (water or soil) samples. Using combinations of filtration, agar plating, and selective agents, leptospires can be isolated in pure cultures even from complex contaminated sources in standard EMJH culture medium.

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Keywords:  Blood; Culture; Filtration; Isolation; Kidney; Leptospira; Selective agents; Soil; Urine; Water

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Year:  2020        PMID： 32632854     DOI： 10.1007/978-1-0716-0459-5_1

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  2 in total

1.  A prospective study to evaluate the accuracy of rapid diagnostic tests for diagnosis of human leptospirosis: Result from THAI-LEPTO AKI study.

Authors:  Janejira Dinhuzen; Umaporn Limothai; Sasipha Tachaboon; Panadda Krairojananan; Bangon Laosatiankit; Sakarin Boonprasong; Nuttha Lumlertgul; Sadudee Peerapornratana; Nattachai Srisawat
Journal:  PLoS Negl Trop Dis       Date:  2021-02-19

2.  First Isolation and Molecular Typing of Pathogenic and Intermediate Leptospira Species from Urine of Symptomatic Dogs.

Authors:  Ivana Piredda; Loris Bertoldi; Giuseppe Benvenuto; Bruna Palmas; Aureliana Pedditzi; Pierangela Pintore; Valentina Chisu
Journal:  Vet Sci       Date:  2021-12-02
  2 in total

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