Jiayuan Liao1,2, Xiangrong Liu1,3, Ang Hu1, Haixing Song1, Xiuzhi Chen2, Zhenhua Zhang4,5,6. 1. Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, College of Resources and Environmental Sciences, Hunan Agricultural University, Changsha, 410128, China. 2. Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies, School of Atmospheric Sciences, Sun Yat-Sen University, Guangzhou, 510275, China. 3. Hengyang Branch of Hunan Tobacco Company, Hengyang, 421600, China. 4. Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, College of Resources and Environmental Sciences, Hunan Agricultural University, Changsha, 410128, China. zhzh1468@163.com. 5. National Engineering Laboratory On Soil and Fertilizer Resources Efficient Utilization, Changsha, 410128, China. zhzh1468@163.com. 6. Hunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resources Use, Hunan Provincial Key Laboratory of Nutrition in Common University, Changsha, China. zhzh1468@163.com.
Abstract
Biochar-based controlled release nitrogen fertilizers (BCRNFs) have received increasing attention due to their ability to improve nitrogen-use efficiency (NUE) and increase crop yields. We previously developed a novel BCRNF, but its effects on soil microbes, NUE, and crop yields have not been reported. Therefore, we designed a pot experiment with five randomised treatments: CK (without urea and biochar), B (addition biochar without urea), B + U (biochar mixed urea), Urea (addition urea without biochar), and BCRNF (addition BCRNF), to investigate the effects of BCRNF on nitrifiers and denitrifiers, and how these impact nitrogen supply and NUE. Results of high-throughput sequencing revealed bacterial community groups with higher nutrient metabolic cycling ability under BCRNF treatment during harvest stage. Compared to Urea treatment, BCRNF treatment stimulated nitrification by increasing the copy number of the bacterial amoA gene and reducing nitrous oxide emission by limiting the abundance of nirS and nirK. Eventually, BCRNF successfully enhanced the yield (~ 16.6%) and NUE (~ 58.79%) of rape by slowly releasing N and modulating the abundance of functional microbes through increased soil nitrification and reduced denitrification, as compared with Urea treatment. BCRNF significantly improved soil NO3-, leading to an increase in N uptake by rape and NUE, thereby promoting rape growth and increasing grain yield.
Biochar-based controlled release n class="Chemical">nitrogen fertilizers (BCRNFs) have received inpan>creasinpan>g attenpan>tionpan> dpan> class="Chemical">ue to their ability to improve nitrogen-use efficiency (NUE) and increase crop yields. We previously developed a novel BCRNF, but its effects on soil microbes, NUE, and crop yields have not been reported. Therefore, we designed a pot experiment with five randomised treatments: CK (without urea and biochar), B (addition biochar without urea), B + U (biochar mixed urea), Urea (addition urea without biochar), and BCRNF (addition BCRNF), to investigate the effects of BCRNF on nitrifiers and denitrifiers, and how these impact nitrogen supply and NUE. Results of high-throughput sequencing revealed bacterial community groups with higher nutrient metabolic cycling ability under BCRNF treatment during harvest stage. Compared to Urea treatment, BCRNF treatment stimulated nitrification by increasing the copy number of the bacterial amoA gene and reducing nitrous oxide emission by limiting the abundance of nirS and nirK. Eventually, BCRNF successfully enhanced the yield (~ 16.6%) and NUE (~ 58.79%) of rape by slowly releasing N and modulating the abundance of functional microbes through increased soil nitrification and reduced denitrification, as compared with Urea treatment. BCRNF significantly improved soil NO3-, leading to an increase in N uptake by rape and NUE, thereby promoting rape growth and increasing grain yield.
Authors: Xin Zhang; Eric A Davidson; Denise L Mauzerall; Timothy D Searchinger; Patrice Dumas; Ye Shen Journal: Nature Date: 2015-11-23 Impact factor: 49.962
Authors: James N Galloway; Alan R Townsend; Jan Willem Erisman; Mateete Bekunda; Zucong Cai; John R Freney; Luiz A Martinelli; Sybil P Seitzinger; Mark A Sutton Journal: Science Date: 2008-05-16 Impact factor: 47.728
Authors: Johannes Harter; Hans-Martin Krause; Stefanie Schuettler; Reiner Ruser; Markus Fromme; Thomas Scholten; Andreas Kappler; Sebastian Behrens Journal: ISME J Date: 2013-09-26 Impact factor: 10.302
Authors: Claudia I Kammann; Hans-Peter Schmidt; Nicole Messerschmidt; Sebastian Linsel; Diedrich Steffens; Christoph Müller; Hans-Werner Koyro; Pellegrino Conte; Stephen Joseph; Joseph Stephen Journal: Sci Rep Date: 2015-06-09 Impact factor: 4.379
Authors: Xue-Yan Liu; Keisuke Koba; Lina A Koyama; Sarah E Hobbie; Marissa S Weiss; Yoshiyuki Inagaki; Gaius R Shaver; Anne E Giblin; Satoru Hobara; Knute J Nadelhoffer; Martin Sommerkorn; Edward B Rastetter; George W Kling; James A Laundre; Yuriko Yano; Akiko Makabe; Midori Yano; Cong-Qiang Liu Journal: Proc Natl Acad Sci U S A Date: 2018-03-14 Impact factor: 11.205
Authors: Ali Raza; Shaghef Ejaz; Muhammad Shahzad Saleem; Vaclav Hejnak; Furqan Ahmad; Mohamed A A Ahmed; Saqer S Alotaibi; Ahmed M El-Shehawi; Moodi Saham Alsubeie; Ali Tan Kee Zuan Journal: PLoS One Date: 2021-12-17 Impact factor: 3.752
Authors: Krzysztof Gondek; Monika Mierzwa-Hersztek; Wojciech Grzymała; Tomasz Głąb; Tomasz Bajda Journal: Materials (Basel) Date: 2021-05-10 Impact factor: 3.623