Xinyu Zhang1, Shenyu Wang2, Guanhong Lin1, Dan Wang1. 1. Department of Integrated TCM & Western Medicine, Liaoning Cancer Hospital &Institute, Shenyang, Liaoning, 110043, China. 2. Department of Integrated TCM & Western Medicine, Liaoning Cancer Hospital &Institute, Shenyang, Liaoning, 110043, China. Electronic address: ixhkci@163.com.
Abstract
BACKGROUND: Circular RNAs (circRNAs) are related to the carcinogenesis of cancers, including glioma. However, the role and mechanism of circRNA pleiotrophin (circ-PTN) remain largely unknown. METHODS: Glioma tissues (n = 30) and normal tissues were obtained. Glioma cell lines LN229 and A172 were cultured for experiments in vitro. circ-PTN, microRNA-432-5p (miR-432-5p) and Ras-related protein Rab-10 (RAB10) levels were examined via quantitative reverse transcription polymerase chain reaction or western blot. Cell proliferation, invasion and glycolysis were examined via 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide, colony formation analysis, transwell invasion analysis, specific glucose, lactate or adenosine triphosphate assay kit and western blot. The relationship of miR-432-5p and circ-PTN or RAB10 was analyzed via dual-luciferase reporter analysis. The effect of circ-PTN on glioma development in vivo was explored by a murine xenograft model. RESULTS: circ-PTN expression was enhanced and miR-432-5p abundance was reduced in glioma tissues and cells. circ-PTN silence suppressed cell proliferation, invasion and glycolysis. circ-PTN regulated glioma development by directly sponging miR-432-5p. RAB10 was a target of miR-432-5p and miR-432-5p inhibited cell proliferation, invasion and glycolysis by targeting RAB10. circ-PTN could modulate RAB10 expression via miR-432-5p. circ-PTN knockdown reduced glioma cell xenograft tumor growth in vivo. CONCLUSION: circ-PTN knockdown repressed cell proliferation, invasion and glycolysis in glioma via modulating miR-432-5p and RAB10.
BACKGROUND: Circular RNAs (circRNAs) are related to the carcinogenesis of cancers, including glioma. However, the role and mechanism of circRNA pleiotrophin (circ-PTN) remain largely unknown. METHODS:Glioma tissues (n = 30) and normal tissues were obtained. Glioma cell lines LN229 and A172 were cultured for experiments in vitro. circ-PTN, microRNA-432-5p (miR-432-5p) and Ras-related protein Rab-10 (RAB10) levels were examined via quantitative reverse transcription polymerase chain reaction or western blot. Cell proliferation, invasion and glycolysis were examined via 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide, colony formation analysis, transwell invasion analysis, specific glucose, lactate or adenosine triphosphate assay kit and western blot. The relationship of miR-432-5p and circ-PTN or RAB10 was analyzed via dual-luciferase reporter analysis. The effect of circ-PTN on glioma development in vivo was explored by a murine xenograft model. RESULTS:circ-PTN expression was enhanced and miR-432-5p abundance was reduced in glioma tissues and cells. circ-PTN silence suppressed cell proliferation, invasion and glycolysis. circ-PTN regulated glioma development by directly sponging miR-432-5p. RAB10 was a target of miR-432-5p and miR-432-5p inhibited cell proliferation, invasion and glycolysis by targeting RAB10. circ-PTN could modulate RAB10 expression via miR-432-5p. circ-PTN knockdown reduced glioma cell xenograft tumor growth in vivo. CONCLUSION:circ-PTN knockdown repressed cell proliferation, invasion and glycolysis in glioma via modulating miR-432-5p and RAB10.