Development of a reliable bioanalytical method based on prostate specific antigen trapping on the cavity of molecular imprinted polymer towards sensing of PSA using binding affinity of PSA-MIP receptor: A novel biosensor.

In this study, electrically-conducting poly [Toluidine Blue (PTB)] was applied as artificial receptor. It was organized by molecular imprinting approaches and via electrochemical technique for the sensitive monitoring of prostate-specific antigen (PSA). The protein-imprinted PTB was electropolymerized in a pre-formed glutaraldehyde-cysteamine (GA-Cys A) matrix on the surface of gold electrode, which significantly boosted the stability against degradation of the Molecular Imprinted Polymer (MIP) on the surface of pre-modified gold electrode. Moreover, the MIP bio-receptor ability towards protein recognition was explored by some electrochemical techniques. The binding affinity of MIP system was considerably upper than that of non-imprinted polymer (NIP) system, indicating the success of the method in generating imprinted materials that was specifically use to PSA protein. The incubation of the MIP modified electrode in various concentration of PSA (from 1-60 μg/L) resulted in the increase of the Fe (CN)63-/4- redox peak current. The bio-device also showed linear response from 1-60 μg/L and LLOQ of 1 μg/L by using DPV technique, leading to PSA monitoring in clinical samples. The proposed MIP-based biosensor was satisfactorily applied to the determination of PSA in human plasma samples. Therefore, the developed bio-device provides a new approach for sensitive, simple, rapid, and cost-effective monitoring of 1 μg/L of PSA. Notably, this approach could appear as an appropriate candidate for point-of-care (POC) use in clinical and biomedical analyses.