Sarah E Deemer1,2, Rachel A H Davis1,2, Brandon M Roberts3, Daniel L Smith1,2, Andrew P Koutnik4, Angela M Poff4, Dominic P D'Agostino4, Eric P Plaisance1,2,3. 1. Department of Nutrition Sciences, University of Alabama at Birmingham, Birmingham, Alabama, USA. 2. Nutrition Obesity Research Center, University of Alabama at Birmingham, Birmingham, Alabama, USA. 3. Department of Human Studies, University of Alabama at Birmingham, Birmingham, Alabama, USA. 4. Molecular Pharmacology and Physiology, University of South Florida, Tampa, Florida, USA.
Abstract
OBJECTIVE: The aim of this study was to examine the effects of a ketone ester (KE)-supplemented diet on energy expenditure (EE) and adiposity in mice housed at 23 °C versus thermoneutrality (30 °C), in which sympathetic nervous system activity is diminished. METHODS: Thirty-two 10-week-old male C57BL/6J mice were assigned to 1 of 4 groups (n = 8 per group): 30% KE diet + 23 °C (KE23), control (CON) diet + 23 °C (CON23), 30% KE diet + 30 °C (KE30), or CON diet + 30 °C (CON30). CON mice were pair-fed to the average intake of mice consuming the KE diet (ad libitum) for 8 weeks. Body composition and components of energy balance were measured at completion of the study. RESULTS: CON23 (mean ± SD, 26.0 ± 1.6 g) and CON30 (29.7 ± 1.4 g) mice weighed more than KE groups (P < 0.03 for both) and were also different from each other (CON23 vs. CON30, P < 0.01). However, KE23 (23.4 ± 2.7 g) and KE30 (23.1 ± 1.9 g) mice were not different in body weight. As expected, food intake at 30 °C (2.0 ± 0.3 g/d) was lower than at 23 °C (2.6 ± 0.3 g/d, P < 0.01). Diet did not influence resting and total EE, but mice housed at 30 °C had lower EE compared with mice at 23 °C (P < 0.01). CONCLUSIONS: Dietary KEs attenuate body weight gain at standard (23 °C) and thermoneutral (30 °C) housing temperatures, and this effect is not mediated by increased EE under these conditions.
OBJECTIVE: The aim of this study was to examine the effects of a ketone ester (KE)-supplemented diet on energy expenditure (EE) and adiposity in mice housed at 23 °C versus thermoneutrality (30 °C), in which sympathetic nervous system activity is diminished. METHODS: Thirty-two 10-week-old male C57BL/6J mice were assigned to 1 of 4 groups (n = 8 per group): 30% KE diet + 23 °C (KE23), control (CON) diet + 23 °C (CON23), 30% KE diet + 30 °C (KE30), or CON diet + 30 °C (CON30). CON mice were pair-fed to the average intake of mice consuming the KE diet (ad libitum) for 8 weeks. Body composition and components of energy balance were measured at completion of the study. RESULTS: CON23 (mean ± SD, 26.0 ± 1.6 g) and CON30 (29.7 ± 1.4 g) mice weighed more than KE groups (P < 0.03 for both) and were also different from each other (CON23 vs. CON30, P < 0.01). However, KE23 (23.4 ± 2.7 g) and KE30 (23.1 ± 1.9 g) mice were not different in body weight. As expected, food intake at 30 °C (2.0 ± 0.3 g/d) was lower than at 23 °C (2.6 ± 0.3 g/d, P < 0.01). Diet did not influence resting and total EE, but mice housed at 30 °C had lower EE compared with mice at 23 °C (P < 0.01). CONCLUSIONS: Dietary KEs attenuate body weight gain at standard (23 °C) and thermoneutral (30 °C) housing temperatures, and this effect is not mediated by increased EE under these conditions.
Authors: Dominic P D'Agostino; Raffaele Pilla; Heather E Held; Carol S Landon; Michelle Puchowicz; Henri Brunengraber; Csilla Ari; Patrick Arnold; Jay B Dean Journal: Am J Physiol Regul Integr Comp Physiol Date: 2013-04-03 Impact factor: 3.619
Authors: Brianna J Stubbs; Pete J Cox; Rhys D Evans; Peter Santer; Jack J Miller; Olivia K Faull; Snapper Magor-Elliott; Satoshi Hiyama; Matthew Stirling; Kieran Clarke Journal: Front Physiol Date: 2017-10-30 Impact factor: 4.566
Authors: Rachel A H Davis; Sarah E Deemer; Jonathan M Bergeron; Jason T Little; Jonathan L Warren; Gordon Fisher; Daniel L Smith; Kevin R Fontaine; Stephanie L Dickinson; David B Allison; Eric P Plaisance Journal: FASEB J Date: 2018-10-10 Impact factor: 5.834
Authors: Sarah E Deemer; Rachel A H Davis; Barbara A Gower; Andrew P Koutnik; Angela M Poff; Stephanie L Dickinson; David B Allison; Dominic P D'Agostino; Eric P Plaisance Journal: Front Nutr Date: 2019-05-01