Mi Kyeong Lee1, Annah B Wyss1, Megan U Carnes2, Marie Richards3, Christine G Parks1, Laura E Beane Freeman4, Peter S Thorne5, David M Umbach6, M Andrea Azcarate-Peril7, Shyamal D Peddada8, Stephanie J London9. 1. Epidemiology Branch, National Institute of Environmental Health Sciences (NIEHS), National Institutes of Health (NIH), Department of Health and Human Services (DHHS), Research Triangle Park, NC. 2. Genomics in Public Health and Medicine Center, Biostatistics and Epidemiology Division, RTI International, Research Triangle Park, NC. 3. Westat, Durham, NC. 4. Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH, DHHS, Rockville, Md. 5. Department of Occupational and Environmental Health, University of Iowa, Iowa City, Iowa. 6. Biostatistics and Computational Biology Branch, NIEHS, NIH, DHHS, Research Triangle Park, NC. 7. Department of Medicine and Microbiome Core, Center for Gastrointestinal Biology and Disease, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC. 8. Department of Biostatistics, University of Pittsburgh, Pittsburgh, Pa. 9. Epidemiology Branch, National Institute of Environmental Health Sciences (NIEHS), National Institutes of Health (NIH), Department of Health and Human Services (DHHS), Research Triangle Park, NC. Electronic address: london2@niehs.nih.gov.
Abstract
BACKGROUND: Bacterial exposure from house dust has been associated with asthma and atopy in children but whether these relationships are present in adults remains unclear. OBJECTIVE: We sought to examine associations of house dust microbiota with adult asthma, atopy, and hay fever. METHODS: Vacuumed bedroom dust samples from the homes of 879 participants (average age, 62 years) in the Agricultural Lung Health Study, a case-control study of asthma nested within a farming cohort, were subjected to 16S rRNA amplicon sequencing to characterize bacterial communities. We defined current asthma and hay fever using questionnaires and current atopy by blood specific IgE level > 0.70 IU/mL to 1 or more of 10 common allergens. We used linear regression to examine whether overall within-sample bacterial diversity differed by outcome, microbiome regression-based kernel association test to evaluate whether between-sample bacterial community compositions differed by outcome, and analysis of composition of microbiomes to identify differentially abundant bacterial taxa. RESULTS: Overall diversity of bacterial communities in house dust was similar by asthma status but was lower (P < .05) with atopy or hay fever. Many individual bacterial taxa were differentially abundant (false-discovery rate, <0.05) by asthma, atopy, or hay fever. Several taxa from Cyanobacteria, Bacteroidetes, and Fusobacteria were more abundant with asthma, atopy, or hay fever. In contrast, several taxa from Firmicutes were more abundant in homes of individuals with adequately controlled asthma (vs inadequately controlled asthma), individuals without atopy, or individuals without hay fever. CONCLUSIONS: Microbial composition of house dust may influence allergic outcomes in adults. Published by Elsevier Inc.
BACKGROUND: Bacterial exposure from house dust has been associated with asthma and atopy in children but whether these relationships are present in adults remains unclear. OBJECTIVE: We sought to examine associations of house dust microbiota with adult asthma, atopy, and hay fever. METHODS: Vacuumed bedroom dust samples from the homes of 879 participants (average age, 62 years) in the Agricultural Lung Health Study, a case-control study of asthma nested within a farming cohort, were subjected to 16S rRNA amplicon sequencing to characterize bacterial communities. We defined current asthma and hay fever using questionnaires and current atopy by blood specific IgE level > 0.70 IU/mL to 1 or more of 10 common allergens. We used linear regression to examine whether overall within-sample bacterial diversity differed by outcome, microbiome regression-based kernel association test to evaluate whether between-sample bacterial community compositions differed by outcome, and analysis of composition of microbiomes to identify differentially abundant bacterial taxa. RESULTS: Overall diversity of bacterial communities in house dust was similar by asthma status but was lower (P < .05) with atopy or hay fever. Many individual bacterial taxa were differentially abundant (false-discovery rate, <0.05) by asthma, atopy, or hay fever. Several taxa from Cyanobacteria, Bacteroidetes, and Fusobacteria were more abundant with asthma, atopy, or hay fever. In contrast, several taxa from Firmicutes were more abundant in homes of individuals with adequately controlled asthma (vs inadequately controlled asthma), individuals without atopy, or individuals without hay fever. CONCLUSIONS: Microbial composition of house dust may influence allergic outcomes in adults. Published by Elsevier Inc.
Entities:
Keywords:
Bacteria; allergy and immunology; asthma; host microbial interactions; microbiome
Authors: Le Duc Huy Ta; Carina Jing Xuan Tay; Christophe Lay; Paola Florez de Sessions; Cheryl Pei Ting Tan; Michelle Jia Yu Tay; Hui Xing Lau; Atiqa Binte Zulkifli; Gaik Chin Yap; Elizabeth Huiwen Tham; Eliza Xin Pei Ho; Anne Eng Neo Goh; Keith M Godfrey; Johan G Eriksson; Jan Knol; Peter D Gluckman; Yap Seng Chong; Jerry Kok Yen Chan; Kok Hian Tan; Kok Wee Chong; Si Hui Goh; Zai Ru Cheng; Bee Wah Lee; Lynette Pei-Chi Shek; Evelyn Xiu Ling Loo Journal: Environ Microbiol Date: 2021-08-12 Impact factor: 5.476