| Literature DB >> 32614452 |
Om Prakash Singh1,2, Genevieve Syn3, Susanne Nylén4, Christian Engwerda5, David Sacks6, Mary E Wilson7, Rajiv Kumar1,8, Jaya Chakravarty1, Shyam Sundar1, Jenefer M Blackwell3,9, Michaela Fakiola9,10.
Abstract
Visceral leishmaniasis (VL; Leishmania donovani) cases produce interferon-γ and tumor necrosis factor in response to soluble leishmanial antigen (SLA) in whole-blood assays. Using transcriptional profiling, we demonstrate the impact of interleukin-10 (IL-10), a cytokine implicated in VL, on this response. SLA stimulation identified 28 differentially expressed genes (DEGs), 17/28 in a single network with TNF as hub. SLA plus anti-IL-10 produced 454 DEGs, 292 in a single network with TNF, IFNG, NFKBIA, IL6, and IL1B as hubs in concert with a remarkable chemokine/cytokine storm. Our data demonstrate the singular effect of IL-10 as a potent immune modulator in VL.Entities:
Keywords: RNA expression; anti; cytokine storm; interleukin-10; soluble leishmania antigen; visceral leishmaniasis; whole-blood assays
Year: 2021 PMID： 32614452 PMCID： PMC7881328 DOI： 10.1093/infdis/jiaa381
Source DB: PubMed Journal: J Infect Dis ISSN： 0022-1899 Impact factor: 5.226