| Literature DB >> 3261298 |
K Sugimura1, K Tsukahara, Y Ueda, K Takeda, Y Habu, I Azuma.
Abstract
The human macrophage-like cell line U937 constitutively produces a lymphocyte blastogenesis inhibitory factor (LBIF). Using fast protein liquid chromatography (FPLC)-chromatofocusing, FPLC-anion-exchange chromatography and high-performance liquid chromatography-gel permeation chromatography, LBIF has been purified to homogeneity. It is a single polypeptide chain and has a molecular weight of 57,000 daltons according to sodium dodecyl sulphate polyacrylamide gel electrophoresis under reducing conditions. LBIF shows pI 4.28-4.56 upon chromatofocusing on Mono P.Entities:
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Year: 1988 PMID： 3261298 DOI： 10.1016/s0021-9673(00)94517-5
Source DB: PubMed Journal: J Chromatogr