Novella M Bates1, Cristina Puy2, Patrick L Jurney2,3, Owen J T McCarty2, Monica T Hinds2. 1. Department of Biomedical Engineering, Oregon Health & Science University, Portland, OR, USA. batesno@ohsu.edu. 2. Department of Biomedical Engineering, Oregon Health & Science University, Portland, OR, USA. 3. Department of Biomedical Engineering, San Jose State University, San Jose, CA, USA.
Abstract
PURPOSE: Crosslinked poly(vinyl alcohol) (PVA) is a biomaterial that can be used for multiple cardiovascular applications. The success of implanted biomaterials is contingent on the properties of the material. A crucial consideration for blood-contacting devices is their potential to incite thrombus formation, which is dependent on the material surface properties. The goal of this study was to quantify the effect of different crosslinking methods of PVA hydrogels on in vitro thrombogenicity. METHODS: PVA was manufactured using three different crosslinking methods: 30% sodium trimetaphosphate (STMP), three 24 h freeze-thaw cycles (FT), and 2% glutaraldehyde-crosslinked (GA) to produce STMP-PVA, FT-PVA and GA-PVA, respectively. Expanded polytetrafluoroethylene (ePTFE) was used as a clinical control. As markers of thrombus formation, the degree of coagulation factor (F) XII activation, fibrin formation, and platelet adhesion were measured. RESULTS: The GA-PVA material increased FXII activation in the presence of cofactors compared to vehicle and increase platelet adhesion compared to other PVA surfaces. The STMP-PVA and FT-PVA materials had equivalent degrees of FXII activation, fibrin formation and platelet adhesion. CONCLUSION: This work supports crosslinker dependent thrombogenicity of PVA hydrogels and advances our understanding of how the manufacturing of a PVA hydrogel affects its hemocompatibility.
PURPOSE: Crosslinked poly(vinyl alcohol) (PVA) is a biomaterial that can be used for multiple cardiovascular applications. The success of implanted biomaterials is contingent on the properties of the material. A crucial consideration for blood-contacting devices is their potential to incite thrombus formation, which is dependent on the material surface properties. The goal of this study was to quantify the effect of different crosslinking methods of PVA hydrogels on in vitro thrombogenicity. METHODS:PVA was manufactured using three different crosslinking methods: 30% sodium trimetaphosphate (STMP), three 24 h freeze-thaw cycles (FT), and 2% glutaraldehyde-crosslinked (GA) to produce STMP-PVA, FT-PVA and GA-PVA, respectively. Expanded polytetrafluoroethylene (ePTFE) was used as a clinical control. As markers of thrombus formation, the degree of coagulation factor (F) XII activation, fibrin formation, and platelet adhesion were measured. RESULTS: The GA-PVA material increased FXII activation in the presence of cofactors compared to vehicle and increase platelet adhesion compared to other PVA surfaces. The STMP-PVA and FT-PVA materials had equivalent degrees of FXII activation, fibrin formation and platelet adhesion. CONCLUSION: This work supports crosslinker dependent thrombogenicity of PVA hydrogels and advances our understanding of how the manufacturing of a PVA hydrogel affects its hemocompatibility.
Authors: Patrick L Jurney; Deirdre E J Anderson; Grace Pohan; Evelyn K F Yim; Monica T Hinds Journal: Macromol Biosci Date: 2018-09-05 Impact factor: 4.979
Authors: Julia M Ino; Ervi Sju; Véronique Ollivier; Evelyn K F Yim; Didier Letourneur; Catherine Le Visage Journal: J Biomed Mater Res B Appl Biomater Date: 2013-07-11 Impact factor: 3.368
Authors: Marie F A Cutiongco; Marek Kukumberg; Jonnathan L Peneyra; Matthew S Yeo; Jia Y Yao; Abdul Jalil Rufaihah; Catherine Le Visage; Jackie Pei Ho; Evelyn K F Yim Journal: Front Bioeng Biotechnol Date: 2016-06-08